Liu, Chenglong Guo, Haoze Zhao, Xinmei Zou, Bingxi Sun, Ting Feng, Jinwei Zeng, Zhiyong Wen, Xueer Chen, Jun Hu, Zhangli
...
Published in
Frontiers in Bioengineering and Biotechnology
Post-transcriptional modification of nucleic acids including transfer RNA (tRNA), ribosomal RNA (rRNA) and messenger RNA (mRNA) is vital for fine-tunning of mRNA translation. Methylation is one of the most widespread post-transcriptional modifications in both eukaryotes and prokaryotes. HsWBSCR22 and ScBUD23 encodes a 18S rRNA methyltransferase tha...
Craig, Rory J Gallaher, Sean D Shu, Shengqiang Salomé, Patrice A Jenkins, Jerry W Blaby-Haas, Crysten E Purvine, Samuel O O'Donnell, Samuel Barry, Kerrie Grimwood, Jane
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Five versions of the Chlamydomonas reinhardtii reference genome have been produced over the last two decades. Here we present version 6, bringing significant advances in assembly quality and structural annotations. PacBio-based chromosome-level assemblies for two laboratory strains, CC-503 and CC-4532, provide resources for the plus and minus matin...
Cardoso Esteves, Sara Marina
Natural variation in living organisms is essential in stress responses and adaptation to new environments. Many of the traits presenting natural variation are regulated by a complex genetic architecture of genes with small to moderate effects. Consequently, understanding which variants impact traits of medical, industrial or agronomical interest is...
Armič, Doroteja
Vakuolni procesivni encimi (VPE) so cisteinske proteaze, ki jih najdemo v rastlinah. Lokalizirani so v vakuolah, kjer cepijo peptidne vezi za asparaginskimi in aspartatnimi aminokislinskimi ostanki. VPE se sintetizirajo v obliki neaktivnih prekurzorjev, ki se avtokatalitsko procesirajo in aktivirajo pri kislem pH. Takšni pogoji so v vakuoli, kjer s...
Chouhan, Nisha Yadav, Ranay Mohan Pandey, Jayendra Subramanyam, Rajagopal
Published in
Biochimica et biophysica acta. Bioenergetics
The localization of carotenoids and macromolecular organization of thylakoid supercomplexes have not been reported yet in Chlamydomonas reinhardtii WT and cyclic electron transport mutants (pgrl1 and pgr5) under high light. Here, the various pigments, protein composition, and pigment-protein interactions were analyzed from the cells, thylakoids, an...
Wang, Quan Luo, Haolin Zhuang, Jieyi Li, Xinyi Huang, Danqiong Hu, Zhangli Zhang, Guiying
Published in
Frontiers in Microbiology
Chlamydomonas reinhardtii is a photosynthetic eukaryote showing great industrial potential. The synthesis and in vivo function of the artificial C. reinhardtii genome not only promotes the development of synthetic biology technology but also supports industries that utilize this algae. Mitochondrial genome (MtG) is the smallest and simplest genome ...
Zheng, Shiyan Zou, Shangyun Wang, Hongyan Feng, Tian Sun, Shourui Chen, Hui Wang, Qiang
Published in
Frontiers in Microbiology
Chlamydomonas reinhardtii is a model strain to explore algal lipid metabolism mechanism, and exhibits great potentials in large-scale production of lipids. Completely lacking nitrogen is an efficient strategy to trigger the lipid synthesis in microalgal cells, while it always leads to the obvious reduction in the biomass. To illustrate the optimal ...
Wichmann, Julian Eggert, Annibel Elbourne, Liam D H Paulsen, Ian T Lauersen, Kyle J Kruse, Olaf
Published in
Microbial cell factories
Eukaryotic algae have recently emerged as hosts for metabolic engineering efforts to generate heterologous isoprenoids. Isoprenoid metabolic architectures, flux, subcellular localization, and transport dynamics have not yet been fully elucidated in algal hosts. In this study, we investigated the accessibility of different isoprenoid precursor pools...
Boček, Žan
Oskrba rane z zadostno količino kisika je ključna za uspešno celjenje, vendar je zaradi poškodb lokalnega krvnega obtoka omejena. Dovajanje kisika se je izboljšalo z enkapsulacijo fotosintetsko aktivnih celic Chlamydomonas reinhardtii v alginatnih hidrogelih z dodano želatino. Fizikalno zamrežen alginat ščiti celice in dovaja hranilne snovi, medtem...
Vogrinec, Lana
Metakaspaze so cisteinske proteaze, ki se na osnovi strukturne homologije s kaspazami uvrščajo v družino C14 klana CD. Najdemo jih pri raznovrstnih organizmih, med drugim pri bakterijah, arhejah, algah, enoceličnih evkariontih in rastlinah, ne pa tudi pri živalih. Poleg kaspazam homolognih domen p20 in p10 lahko vsebujejo tudi nekatere dodatne regi...
