HeLa cells were found to enter and complete mitosis in the presence of puromycin or actinomycin D. Daughter cells formed in the presence of puromycin contained completed nuclear membranes. Daughter cells resumed protein synthesis shortly after completing mitosis however, this resumption of protein synthesis could be prevented by actinomycin D. Data...
We have compared the biochemical capacity of cultured cells from a variety of animal sources to replicate the genetic information of two small RNA viruses. Mengovirus is shown to infect and kill cultured cells from many animal species regardless of whether the virus replicated well, moderately, or poorly in any cell type. The production of infectio...
Specific aminoacyl-tRNA's from different mammalian organs and species have been compared by methylated albumin column chromatography. The elution profile of such chromatographs were usually similar, both between tRNA's from different organs in the same animal, and across widely divergent mammalian species. Species differences were found in the elut...
WE have reported that cell contact per se is not sufficient to explain the rapid and dramatic in vitro destruction of target L cells observed when they are treated with immune lymphocytes or non-immune phytohaemagglutinin (PHA)-stimulated lymphocytes derived from various inbred mice1. Instead, cell contact is apparently involved in the induction of...
1. Cerebral RNA of adult and newborn rats was labelled in vivo by intracervical injection of [5-(3)H]uridine or [(32)P]phosphate. Hepatic RNA of similar animals was labelled by intraperitoneal administration of [6-(14)C]orotic acid. Nuclear and cytoplasmic fractions were isolated and purified by procedures involving extraction with phenol and repea...
This communication describes some of the physical and chemical characteristics of mouse lymphotoxin (MLT), a cytotoxic factor released by mouse lymphocytes in vitro after stimulation with phytohemagglutinin. Mouse LT activity was found to be heat and pH stable exhibiting properties characteristic of a protein having a molecular weight of approximat...
