Zhang, Yue Lanjuin, Anne Chowdhury, Suvagata Roy Mistry, Meeta Silva-García, Carlos G Weir, Heather J Lee, Chia-Lin Escoubas, Caroline C Tabakovic, Emina Mair, William B
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Published in
eLife
Target of rapamycin complex 1 (TORC1) and AMP-activated protein kinase (AMPK) antagonistically modulate metabolism and aging. However, how they coordinate to determine longevity and if they act via separable mechanisms is unclear. Here, we show that neuronal AMPK is essential for lifespan extension from TORC1 inhibition, and that TORC1 suppression ...
Ganner, Athina Gerber, Julia Ziegler, Anna-Katharina Li, Yujie Kandzia, Jakob Matulenski, Tanja Kreis, Saskia Breves, Gerhard Klein, Marinella Walz, Gerd
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Published in
Experimental gerontology
SKN-1/Nrf transcription factors regulate diverse biological processes essentially stress defense, detoxification, and longevity. Studies in model organisms have identified a broad range of regulatory processes and mechanisms that profoundly influence SKN-1/Nrf functions. Defining the mechanisms how SKN-1 is regulated will provide insight how cells ...
Shao, Zhenhua Niwa, Shinsuke Higashitani, Atsushi Daigaku, Yasukazu
Published in
DNA repair
In eukaryotes, the DNA damage bypass pathway is promoted by ubiquitylation of PCNA at the conserved lysine 164. Using CRISPR-Cas9 system, we introduced amino acid substitution at K165 of C. elegans PCNA that corresponds to K164 in other characterised organisms and examined the contribution of this residue at a variety of stages during development. ...
Qiu, Chen Bhat, Vandita D Rajeev, Sanjana Zhang, Chi Lasley, Alexa E Wine, Robert N Campbell, Zachary T Hall, Traci M Tanaka
Published in
eLife
In the Caenorhabditis elegans germline, fem-3 Binding Factor (FBF) partners with LST-1 to maintain stem cells. A crystal structure of an FBF-2/LST-1/RNA complex revealed that FBF-2 recognizes a short RNA motif different from the characteristic 9-nt FBF binding element, and compact motif recognition coincided with curvature changes in the FBF-2 scaf...
Philip, Nicholas S Escobedo, Fernando Bahr, Laura L Berry, Brandon J Wojtovich, Andrew P
Published in
G3 (Bethesda, Md.)
The introduction of exogenous genes in single-copy at precise genomic locations is a powerful tool that has been widely used in the model organism Caenorhabditis elegans Here, we have streamlined the process by creating a rapid, cloning-free method of single-copy transgene insertion we call Mos1 element-mediated CRISPR integration (mmCRISPi). The p...
Sakai, Yoshiki Hanafusa, Hiroshi Pastuhov, Strahil Iv Shimizu, Tatsuhiro Li, Chun Hisamoto, Naoki Matsumoto, Kunihiro
Published in
EMBO reports
In Caenorhabditis elegans, the JNK MAP kinase (MAPK) pathway is important for axon regeneration. The JNK pathway is activated by a signaling cascade consisting of the growth factor SVH-1 and its receptor tyrosine kinase SVH-2. Expression of the svh-2 gene is induced by axonal injury in a process involving the transcription factors ETS-4 and CEBP-1....
Pradhan, Diana Negi, Vidya Devi
Published in
Microbial pathogenesis
Adaptation is an important phenomenon for the survival of any organism in various in-vitro and in-vivo conditions leading to their better survival. We studied Salmonella, for the evolutionary adaptation under different conditions. When Salmonella was passaged for 12 rounds in Luria Bertani (P12-STM) media, F media (F12-STM) and Caenorhabditis elega...
Huang, Yung-Chi Pirri, Jennifer K. Rayes, Diego Gao, Shangbang Mulcahy, Ben Grant, Jeff Saheki, Yasunori Francis, Michael M. Zhen, Mei Alkema, Mark J.
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Mutations in pre-synaptic voltage gated calcium channels can lead to familial hemiplegic migraine type 1 (FHM1). While mammalian studies indicate that the migraine brain is hyperexcitable due to enhanced excitation or reduced inhibition, the molecular and cellular mechanisms underlying this excitatory/inhibitory (E/I) imbalance are poorly understoo...
Pryor, R Norvaisas, P Marinos, G Best, L Bruun Thingholm, L Quintaneiro, L De Haes, W Esser, D Waschina, S Lujan, C
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Metformin is the first-line therapy for treating type-2 diabetes and a promising anti-aging drug. We set out to address the fundamental question of how gut microbes and nutrition, key regulators of host physiology, impact the effects of metformin. Combining two tractable genetic models, the bacterium E. coli and the nematode C. elegans, we develope...
Smith, Latasha L Ryde, Ian T Hartman, Jessica H Romersi, Riccardo F Markovich, Zachary Meyer, Joel N
Published in
Neurotoxicology
In order to develop a better understanding of the role environmental toxicants may play in the onset and progression of neurodegenerative diseases, it has become increasingly important to optimize sensitive methods for quickly screening toxicants to determine their ability to disrupt neuronal function. The nematode Caenorhabditis elegans can help w...