Published in Journal of bioscience and bioengineering
Microcarriers provide a high surface-area-to-volume ratio that can realize high yields of cell products, including human mesenchymal stem cells (hMSCs). Here, we report a novel poly(vinyl alcohol) (PVA)-based microcarrier for hMSC expansion in suspension culture. PVA microcarriers were prepared as collagen-coated PVA hydrogels 181 μm in size and a ...
Published in Bioresource technology
Resolvin D5 (RvD5), 7S,17S-dihydroxy-4Z,8E,10Z,13Z,15E,19Z-docosahexaenoic acid (DHA) is a specialized pro-resolving mediator (SPM) generated in human macrophages. It is implicated in the resolution of inflammation and synthesized using an inefficient chemical process. Here, DHA-enriched oil hydrolysate was prepared from oils by lipase with resin t...
L'abstract è presente nell'allegato / the abstract is in the attachment
Published in Journal of environmental management
Precipitation, evapotranspiration (ET), waste tonnage, landfill gas (LFG), and leachate data were aggregated from public sources to perform a 5-10 year water balance and estimate the contributions of three water sources (precipitation, incoming waste, and leachate recycling) for 36 active municipal solid waste (MSW) landfills in Ohio, USA. Uniquely...
[ES] El presente trabajo se enfoca en el desarrollo e implementación de un sistema de control industrial para el control de un biorreactor destinado a la producción de etanol. Dado el nivel de complejidad del proceso, se ha optado por utilizar un enfoque basado en un gemelo digital. El proyecto se divide en varias fases fundamentales: 1. Implementa...
Published in Enzyme and microbial technology
Alternative cell factories, such as the unicellular ciliate eukaryotic Tetrahymena thermophila, may be required for the production of protein therapeutics that are challenging to produce in conventional expression systems. T. thermophila (Tt) can secrete proteins with the post-translational modifications necessary for their function in humans. In t...
Published in Chemosphere
Sustainable energy transition has brought the attention towards microalgae utilization as potential feedstock due to its tremendous capabilities over its predecessors for generating more energy with reduced carbon footprint. However, the commercialization of microalgae feedstock remains debatable due to the various factors and considerations taken ...
Published in Protein expression and purification
Apolipoprotein (apo) E functions in lipoprotein metabolism as a low density lipoprotein receptor ligand. ApoE is comprised of two structural domains, a 22 kDa N-terminal (NT) domain that adopts a helix bundle conformation and a 10 kDa C-terminal domain with strong lipid binding affinity. The NT domain is capable of transforming aqueous phospholipid...
Published in Cellular and molecular bioengineering
In the colorectal cancer (CRC) tumor microenvironment, cancerous and precancerous cells continuously experience mechanical forces associated with peristalsis. Given that mechanical forces like shear stress and strain can positively impact cancer progression, we explored the hypothesis that peristalsis may also contribute to malignant progression in...
Pichia pastoris is a widely used recombinant protein expression system, so much that to date, more than 500 biomolecules have been synthesized using this yeast, many of which are approved for production and commercialization by the biotechnological industry. The pAOX1-based expression system (MUT+) is one of the most widely used to produce rProt in...
