Crowder, R J Freeman, R S
Published in
The Journal of neuroscience : the official journal of the Society for Neuroscience
Recent studies have suggested a role for phosphatidylinositol (PI) 3-kinase in cell survival, including the survival of neurons. We used rat sympathetic neurons maintained in vitro to characterize the potential survival signals mediated by PI 3-kinase and to test whether the Akt protein kinase, a putative effector of PI 3-kinase, functions during n...
Mok, CL Gil-Gomez, G Williams, O Coles, M Taga, S Tolaini, M Norton, T Kioussis, D Brady, HJM
Bad is a distant relative of Bcl-2 and acts to promote cell death. Here, we show that Bad expression levels are greatly increased in thymocytes during apoptosis. We generated bad transgenic mice to study the action of upregulated Bad expression on T cell apoptosis. The T cells from these mice are highly sensitive to apoptotic stimuli, including ant...
Chin, Lawrence S. Murray, Susan F. Harter, David H. Doherty, Patrick F. Singh, Satyendra K.
Published in
Journal of Biomedical Science
The dual signal hypothesis of apoptosis holds that a common signal can activate both apoptotic and proliferative pathways. The fate of a cell is dependent on which of these two pathways predominates. In the MAPK family of kinases, ERK and JNK have been proposed to mediate apoptosis whereas the PI3K-stimulated kinase, Akt/PKB, has been shown to inhi...
Mok, C L Gil-Gómez, G Williams, O Coles, M Taga, S Tolaini, M Norton, T Kioussis, D Brady, H J
Published in
The Journal of experimental medicine
Bad is a distant relative of Bcl-2 and acts to promote cell death. Here, we show that Bad expression levels are greatly increased in thymocytes during apoptosis. We generated bad transgenic mice to study the action of upregulated Bad expression on T cell apoptosis. The T cells from these mice are highly sensitive to apoptotic stimuli, including ant...
Chin, Lawrence S. Murray, Susan F. Harter, David H. Doherty, Patrick F. Singh, Satyendra K.
Published in
Journal of Biomedical Science
The dual signal hypothesis of apoptosis holds that a common signal can activate both apoptotic and proliferative pathways. The fate of a cell is dependent on which of these two pathways predominates. In the MAPK family of kinases, ERK and JNK have been proposed to mediate apoptosis whereas the PI3K-stimulated kinase, Akt/PKB, has been shown to inhi...
Hines, Susan J. Litz, Julie S. Krystal, Geoffrey W.
Published in
Breast Cancer Research and Treatment
Kit, a tyrosine kinase growth factor receptor, and its ligand, stem cell factor (SCF), are commonly coexpressed in breast cancer. We have previously shown that MCF7 cells (that naturally express SCF) transfected with a c-kit expression vector exhibit enhanced growth in serum-free medium supplemented with IGF-1. Consequently, we wished to examine th...
Aikin, Reid Rosenberg, Lawrence Maysinger, Dusica
Published in
Biochemical and Biophysical Research Communications
The isolation of islet cells from the pancreas by enzymatic digestion causes many of these cells to undergo apoptosis. The aim of this work was to investigate the role of phosphatidylinositol 3-kinase (PI3-K)/Akt signaling in mediating the survival of isolated islets. Insulin-like growth factor-1 (IGF-I) was examined as a potential culture media su...
Mo, Hyo-Jung Lee, Hong-Chang Choi, Heung-Soo Yang, Sung-Il
Published in
Biochemical and Biophysical Research Communications
The immediate effect of a 15-min heat shock was examined on the level and the activity of Akt. Following heat shock, the Akt level decreased by 15–70% in a temperature-dependent and phosphorylation status-independent manner. This decrease of Akt level was not prevented by caspase inhibitors. At 48°C, the extent of the breakdown was so immense that ...
Mockridge, James W. Benton, Eleanor C. Andreeva, Larisa V. Latchman, David S. Marber, Michael S. Heads, Richard J.
Published in
Biochemical and Biophysical Research Communications
In this study we have determined the ability of IGF-1 to protect cardiac fibroblasts against osmotic-induced apoptosis and investigated the potential mechanism(s) underlying this protection. Treatment with IGF-1 (1–100 ng/ml) promoted a dose dependent increase in cell survival against osmotic cell death. Both Akt and ERK1/2 were rapidly phosphoryla...
Törőcsik, Beáta Szeberényi, József
Published in
Biochemical and Biophysical Research Communications
Survival and differentiation of PC12 cells depend on the proper balance between the activities of several mitogen-activated protein kinase (MAPK) pathways. We have previously shown that low, nontoxic doses of anisomycin stimulated these MAPKs as well as the expression of several early-response genes and inhibited NGF-induced neurite formation. In t...
