Yi, Guo-Zhong Liu, Ya-Wei Xiang, Wei Wang, Hai Chen, Zi-Yang Xie, Si-di Qi, Song-Tao
Published in
Journal of the neurological sciences
Glioblastoma is one of the most lethal cancers in central nervous system, and some individual cells that cannot be isolated for surgical resection and also show treatment-resistance induce poor prognosis. Hence, in order to research these cells, we treated temozolomide (TMZ)-sensitive U87MG cells with 400μM TMZ in culture media for over 6months and...
Costa, Barbara Dangate, Milind Vetro, Maria Donvito, Giulia Gabrielli, Luca Amigoni, Loredana Cassinelli, Giuliana Lanzi, Cinzia Ceriani, Michela De Gioia, Luca
...
Published in
Bioorganic & medicinal chemistry
The serine-threonine protein kinase Akt, also known as protein kinase B, is a key component of the phosphoinositide 3-kinase (PI3K)-Akt-mTOR axis. Deregulated activation of this pathway is frequent in human tumors and Akt-dependent signaling appears to be critical in cell survival. PI3K activation generates 3-phosphorylated phosphatidylinositols th...
Zou, Ying Fan, Guobiao Wang, Xuemin
Published in
Biochemical and biophysical research communications
The present study aims to investigate the anti-melanoma activity by an Akt1 specific inhibitor A-674563. We showed that A-674563 was anti-proliferative and cytotoxic when added to human melanoma cells (A375, WM-115 and SK-Mel-2 lines). A-674563 induced caspase-dependent apoptotic death of human melanoma cells, and its cytotoxicity was inhibited wit...
Chae, Young Chan Vaira, Valentina Caino, M Cecilia Tang, Hsin-Yao Seo, Jae Ho Kossenkov, Andrew V Ottobrini, Luisa Martelli, Cristina Lucignani, Giovanni Bertolini, Irene
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Published in
Cancer cell
Hypoxia is a universal driver of aggressive tumor behavior, but the underlying mechanisms are not completely understood. Using a phosphoproteomics screen, we now show that active Akt accumulates in the mitochondria during hypoxia and phosphorylates pyruvate dehydrogenase kinase 1 (PDK1) on Thr346 to inactivate the pyruvate dehydrogenase complex. In...
Cho, Christina Horzempa, Carol Jones, David McKeown-Longo, Paula J.
Published in
BMC Cancer
BackgroundFibronectin is a mechanically sensitive protein which is organized in the extracellular matrix as a network of interacting fibrils. The lung tumor stroma is enriched for fibronectin which is thought to contribute to metastasis and drug resistance. Fibronectin is an elastic, multi-modular protein made up of individually folded domains, som...
Yan, Yan Eipper, Betty A Mains, Richard E
Published in
Neuropharmacology
Exogenous brain-derived neurotrophic factor (BDNF), acting through TrkB, is known to promote neurite formation and branching. This response to BDNF was eliminated by inhibition of TrkB kinase and by specific inhibition of the GEF1 domain of Kalirin, which activates Rac1. Neurons from Kalrn knockout mice were unable to activate Rac1 in response to B...
Vandresen-Filho, S. Martins, W. C. Bertoldo, D. B. Rieger, D. K. Maestri, M. Leal, R. B. Tasca, C. I.
Published in
Neurochemical Research
Statins have been shown to promote neuroprotection in a wide range of neurological disorders. However, the mechanisms involved in such effects of statins are not fully understood. Quinolinic acid (QA) is a neurotoxin that induces seizures when infused in vivo and promotes glutamatergic excitotoxicity in the central nervous system. The aim of this s...
Shimizu, Ippei Minamino, Tohru
Published in
Journal of molecular and cellular cardiology
The heart must continuously pump blood to supply the body with oxygen and nutrients. To maintain the high energy consumption required by this role, the heart is equipped with multiple complex biological systems that allow adaptation to changes of systemic demand. The processes of growth (hypertrophy), angiogenesis, and metabolic plasticity are crit...
Pfalzer, Anna C Kamanu, Frederick K Parnell, Laurence D Tai, Albert K Liu, Zhenhua Mason, Joel B Crott, Jimmy W
Published in
Physiological genomics
Obesity is a significant risk factor for colorectal cancer (CRC); however, the relative contribution of high-fat (HF) consumption and excess adiposity remains unclear. It is becoming apparent that obesity perturbs both the intestinal microbiome and metabolome, and each has the potential to induce protumorigenic changes in the epithelial transcripto...
Abuhagr, Ali M MacLea, Kyle S Mudron, Megan R Chang, Sharon A Chang, Ernest S Mykles, Donald L
Published in
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
Molting in decapod crustaceans is controlled by molt-inhibiting hormone (MIH), an eyestalk neuropeptide that suppresses production of ecdysteroids by a pair of molting glands (Y-organs or YOs). Eyestalk ablation (ESA) activates the YOs, which hypertrophy and increase ecdysteroid secretion. At mid premolt, which occurs 7-14days post-ESA, the YO tran...
