Melrose, James
Published in
Methods in molecular biology (Clifton, N.J.)
Composite agarose-polyacrylamide gel electrophoresis (CAPAGE) in gels of 1.2% w/v polyacrylamide and 0.6% w/v agarose can be used to examine the heterogeneity of full-length native proteoglycan populations and their fragments in crude tissue extracts, and when used in conjunction with immunoblotting and specific antibodies to proteoglycan core prot...
Gramegna Tota, Chiara Leone, Alessandra Rossi, Antonio Paganini, Chiara
Published in
Methods in molecular biology (Clifton, N.J.)
Several experimental protocols are available to study the synthesis and secretion of proteoglycans in health and diseases, but there are few methods to analyse the intracellular processing of these macromolecules. We report a western blot analysis on medium and cell layer of primary chondrocyte culture to determine the glycanation status of aggreca...
Lépine, Matthieu Douceau, Sara Devienne, Gabrielle Prunotto, Paul Lenoir, Sophie Regnauld, Caroline Pouettre, Elsa Piquet, Juliette Lebouvier, Laurent Hommet, Yannick
...
Published in
BMC Biology
Background Perineuronal nets (PNNs) are specialized extracellular matrix structures mainly found around fast-spiking parvalbumin (FS-PV) interneurons. In the adult, their degradation alters FS-PV-driven functions, such as brain plasticity and memory, and altered PNN structures have been found in neurodevelopmental and central nervous system disorde...
Zhang, Kai Gao, Lei Wang, Hai-Xu Ye, Lei Shi, Yan-Yan Yang, Wu-Yan Li, Ya-Nan Li, Yan
Published in
Immunological investigations
The present study was designed to identify and understand the potential effectiveness of therapeutic target in intervertebral disc degeneration (IVDD) and its regulation mechanism. The role and mechanism of interleukin-18 (IL-18) in the disease were investigated. The IVDD degenerative nucleus pulposus (NP) tissues from the human and mouse models we...
Bayındır, Şeyma Yılmaz Asan, Canay Demirbaş, Ahmet Emin Keti, Didem Barlak Kütük, Nükhet
Published in
Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery
The aim of this study was to investigate the effect various mediators in synovial fluid (SF) on the pathogenesis of temporomandibular disorders (TMD) and to evaluate the relationship between clinical and radiological features of temporomandibular joint (TMJ) diseases. Patients who had received SF sample during arthrocentesis because of TMD were inc...
Nia, Hadi Tavakoli Ortiz, Christine Grodzinsky, Alan
Published in
Methods in molecular biology (Clifton, N.J.)
Aggrecan, the most abundant extracellular proteoglycan in cartilage (~35% by dry weight), plays a key role in the biophysical and biomechanical properties of cartilage. Here, we review several approaches based on atomic force microscopy (AFM) to probe the physical, mechanical, and structural properties of aggrecan at the molecular level. These appr...
Liu, Jiuxiang Zuo, Qiang Li, Zhi Chen, Jiangqi Liu, Feng
Published in
Molecular immunology
Chondrocyte dysregulation plays a critical role in the development of osteoarthritis (OA). The pro-inflammatory cytokine interleukin-1β (IL-1β) activates chondrocytes and degrades the structural extracellular matrix (ECM). These events are the important mechanism of OA. Trelagliptin, a selective inhibitor of dipeptidyl Peptidase 4 (DPP-4) used for ...
Lai, Yen-Yu Li, Deng Chang, Shu-Wei
Published in
Computational and Structural Biotechnology Journal
Articular cartilage is connective tissue that forms a slippery load-bearing joint surface between bones. With outstanding mechanical properties, it plays an essential role in cushioning impact and protecting the ends of bones. Abnormal mechanical stimulation, such as repetitive overloading or chondral injury, induces excessive cartilage extracellul...
Toscano, Paolo Di Meglio, Lavinia Lonardo, Fortunato Di Meglio, Letizia Mazzarelli, Laura Letizia Sica, Carmine Di Meglio, Aniello
Published in
BMC Pregnancy and Childbirth
BackgroundHeterozygous mutations of the ACAN gene are a major cause of different evolutive growth defects in the pediatric population, but were never described as a cause of fetal skeletal dysplasia.Case presentationA G1 at 21w + 3d came to our institution for the second-trimester ultrasound and a skeletal dysplasia with prevalent involvement of li...
Fülber, Joice Agreste, Fernanda R Seidel, Sarah R T Sotelo, Eric D P Barbosa, Ângela P Michelacci, Yara M Baccarin, Raquel Y A
Published in
World Journal of Stem Cells
BACKGROUND Mesenchymal stem cells (MSCs) represent a promising therapy for the treatment of equine joint diseases, studied due to their possible immunomodulatory characteristics and regenerative capacity. However, the source of most suitable MSCs for producing cartilage for regenerative processes in conjunction with biomaterials for an enhanced fun...
