Renes, Judith S. Reedijk, Ardine M.J. Losekoot, Monique Kant, Sarina G. Van der Steen, Manouk Van der Kaay, Danielle C.M. Hokken-Koelega, Anita C.S. Van Duyvenvoorde, Hermine A. de Bruin, Christiaan
Published in
Hormone Research in Paediatrics
Introduction: Heterozygous variants in the ACAN gene may underlie disproportionate short stature with characteristically accelerated bone age (BA) maturation and/or early-onset osteoarthritis (OA). Methods: The objective of this study was to describe phenotype, analyze genotype-phenotype correlations, and assess the response of growth hormone (GH) ...
Han, Jing Deng, Huan Li, Yang Qiao, Lichun Jia, Hongrui Zhang, Lan Wang, Linghang Qu, Chengjuan
Published in
Carbohydrate polymers
Cartilage repair is a significant clinical issue due to its restricted ability to regenerate and self-heal after cartilage lesions or degenerative disease. Herein, a nano-elemental selenium particle (chondroitin sulfate A‑selenium nanoparticle, CSA-SeNP) is developed by the supramolecular self-assembly of Na2SeO3 and negatively charged chondroitin ...
Novak, Sanja Kalajzic, Ivo
Published in
Bone
Aggrecan (Acan) is a large proteoglycan molecule constituting the extracellular matrix of cartilage, secreted by chondrocytes. To specifically target the chondrocyte lineage, researchers have widely used the AcanCreER mouse model. Evaluation of specificity and efficiency of recombination, requires Cre animals to be crossed with reporter mice. In or...
Melrose, James
Published in
Methods in molecular biology (Clifton, N.J.)
Composite agarose-polyacrylamide gel electrophoresis (CAPAGE) in gels of 1.2% w/v polyacrylamide and 0.6% w/v agarose can be used to examine the heterogeneity of full-length native proteoglycan populations and their fragments in crude tissue extracts, and when used in conjunction with immunoblotting and specific antibodies to proteoglycan core prot...
Gramegna Tota, Chiara Leone, Alessandra Rossi, Antonio Paganini, Chiara
Published in
Methods in molecular biology (Clifton, N.J.)
Several experimental protocols are available to study the synthesis and secretion of proteoglycans in health and diseases, but there are few methods to analyse the intracellular processing of these macromolecules. We report a western blot analysis on medium and cell layer of primary chondrocyte culture to determine the glycanation status of aggreca...
Lépine, Matthieu Douceau, Sara Devienne, Gabrielle Prunotto, Paul Lenoir, Sophie Regnauld, Caroline Pouettre, Elsa Piquet, Juliette Lebouvier, Laurent Hommet, Yannick
...
Published in
BMC biology
Perineuronal nets (PNNs) are specialized extracellular matrix structures mainly found around fast-spiking parvalbumin (FS-PV) interneurons. In the adult, their degradation alters FS-PV-driven functions, such as brain plasticity and memory, and altered PNN structures have been found in neurodevelopmental and central nervous system disorders such as ...
Larsson, Staffan Lohmander, L. Stefan Struglics, André
Published in
Osteoarthritis and Cartilage Open
Objective To determine biological variation of the aggrecanase-generated aggrecan ARGS neoepitope in serum (sARGS) and synovial fluid (sfARGS) within and between patients with osteoarthritis (OA) or anterior cruciate ligament (ACL) injury. Design Matched samples of serum and synovial fluid were available, as parts of clinical trials, from i) 16 sub...
Zhang, Kai Gao, Lei Wang, Hai-Xu Ye, Lei Shi, Yan-Yan Yang, Wu-Yan Li, Ya-Nan Li, Yan
Published in
Immunological investigations
The present study was designed to identify and understand the potential effectiveness of therapeutic target in intervertebral disc degeneration (IVDD) and its regulation mechanism. The role and mechanism of interleukin-18 (IL-18) in the disease were investigated. The IVDD degenerative nucleus pulposus (NP) tissues from the human and mouse models we...
Bayındır, Şeyma Yılmaz Asan, Canay Demirbaş, Ahmet Emin Keti, Didem Barlak Kütük, Nükhet
Published in
Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery
The aim of this study was to investigate the effect various mediators in synovial fluid (SF) on the pathogenesis of temporomandibular disorders (TMD) and to evaluate the relationship between clinical and radiological features of temporomandibular joint (TMJ) diseases. Patients who had received SF sample during arthrocentesis because of TMD were inc...
Nia, Hadi Tavakoli Ortiz, Christine Grodzinsky, Alan
Published in
Methods in molecular biology (Clifton, N.J.)
Aggrecan, the most abundant extracellular proteoglycan in cartilage (~35% by dry weight), plays a key role in the biophysical and biomechanical properties of cartilage. Here, we review several approaches based on atomic force microscopy (AFM) to probe the physical, mechanical, and structural properties of aggrecan at the molecular level. These appr...
