Peterson, Theresa C. Gonzalez, Frank J. Nebert, Daniel W.
Published in
Biochemical Pharmacology
The murine P 1450 and P 3450 genes and flanking regions contain 14 and 15 Msp I sites (C-C-G-G-), respectively, designated M1 through M14 or M15. These two genes from mouse Hepa-1 wild-type (wt) parent and three mutant cell lines were studied for methylation differences with use of the isoschizomers Msp I and Hpa II. The mutant lines included: cl, ...
Robinson, Richard C. Cheng, Kuo-Chi Park, Sang S. Gelboin, Harry V. Freidman, Fred K.
Published in
Biochemical Pharmacology
A pulmonary cytochrome P-450 was purified from lung microsomes of 3-methylcholanthrene (MC)-treated rats by immunoaffinity chromatography using a monoclonal antibody to MC-induced rat liver cytochrome P-450. The isolated pulmonary cytochrome P-450 was MC-inducible and had an apparent molecular weight of 57 kD on sodium dodecyl sulfate-polyacrylamid...
Pike, Susan F. Shephard, Elizabeth A. Rabin, Brian R. Phillips, Ian R.
Published in
Biochemical Pharmacology
We have previously shown that the 43-fold induction by phenobarbital of the major phenobarbital-inducible cytochrome P-450 of rat liver microsomal membranes (PB P-450) is mediated by a 20-fold increase in the amount of its mRNA in the cytoplasm. Here we demonstrate that the induction of the mRNA can be almost entirely accounted for by an increase i...
Schut, Herman A.J. Dixit, Rakesh Loeb, Timothy R. Stoner, Gary D.
Published in
Biochemical Pharmacology
The elimination of metabolism of a single dose (100 mg/kg) of 2,4-dinitrotoluene (2,4-DNT) in A/J mice were examined. After intraperitoneal administration, elimination was rapid, with 70% of the dose appearing in the urine within 4 hr. Four hours after oral administration, only 28.5% of the dose was excreted in the urine, which increased to 66% aft...
Poellinger, Lorenz Lund, Johan Dahlberg, Erik Gustafsson, Jan-Åke
Published in
Analytical Biochemistry
A “batch” hydroxylapatite assay for the 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD) receptor that does not require detergents is described. The receptor could be assayed in rat target tissues using either of the cytochrome P 1-450 inducers [ 3H]TCDD or [ 3H]3-methylcholanthrene as radioligands. A phosphate buffer washing procedure was developed on ...
Jaiswal, Anil K. Nebert, Daniel W. Eisen, Howard W.
Published in
Biochemical Pharmacology
The human MCF-7 and the mouse Hepa-1 cell culture lines were compared for aryl hydrocarbon hydroxylase and acetanilide 4-hydroxylase inducibility by 2,3,7,8-tetrachlorodibenzo- p-dioxine (TCDD) and benzo[ a]anthracene (BA) and TCDD- and BA-specific in cytosol and nucleus. The effective concentration of BA in the growth medium required to induce eit...
Nebert, Daniel W. Eisen, Howard J. Hankinson, Oliver
Published in
Biochemical Pharmacology
The murine Ah locus controls the induction of at least four drug-metabolizing enzymes: cytochromes P 1-450, P 2-450, and P 3-450, and UDP-glucuronosyltransferase. The Ah gene codes for a cytosolic receptor. It is known that the induction response includes: (i) high-affinity binding of specific foreign chemicals to the Ah receptor; (ii) temperature-...
Kitchin, Kirk T.
Published in
Biochemical Pharmacology
The effects of cumene hydroperoxide on microsomal mixed-function oxidase components and enzyme activities were determined. In vitro cumene hydroperoxide treatment decreased cytochrome P-450 content, benzphetamine N-demethylase activity and aryl hydrocarbon hydroxylase activity of hepatic and renal microsomes from adult male and female rats, and of ...