Manassero, Mathieu Paquet, Joseph Deschepper, Mickael Viateau, Véronique Retortillo, Jose Morad Bensidhoum Delphine Logeart-Avramoglou Hervé Petite
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Tissue Engineering Part A
Tissue constructs containing mesenchymal stem cells (MSCs) are appealing strategies for repairing large segmental bone defects, but they do not allow consistent bone healing and early cell death was identified as a cause of failure. However, little is known about cell survival in the clinical microenvironment encountered during bone healing process...
Baudequin, Timothée Bedoui, Fahmi Dufresne, Murielle Paullier, Patrick Legallais, Cécile
We designed a sheet-like bone substitute capable of adapting to different geometries and becoming a standard tissue-engineered process for bone surgery. Preosteoblastic cells were seeded on to a monolayer of calcium phosphate granules and cultured in a flat parallelepipedic cell culture chamber for one month. From the various diameters of the granu...
Devin Neal Mahmut Selman Sakar Rashid Bashir Vincent Chan Haruhiko Harry Asada
Published in
Tissue Engineering Part A
Esther Potier Ito, Keita
Published in
Tissue Engineering Part A
Bone marrow stromal cells (BMSCs) have shown promising potential to stop intervertebral disc degeneration in several animal models. In order to restore a healthy state, though, this potential should be further stimulated. Notochordal cells (NCs), influential in disc development, have been shown to stimulate BMSC differentiation, but it is unclear h...
Monfoulet, Laurent-Emmanuel Becquart, Pierre Marchat, David Vandamme, Katleen Bourguignon, Marianne Pacard, Elodie Viateau, Véronique Hervé Petite Delphine Logeart-Avramoglou
Published in
Tissue Engineering Part A
The present study aimed at elucidating the effect of local pH in the extracellular microenvironment of tissue-engineered (TE) constructs on bone cell functions pertinent to new tissue formation. To this aim, we evaluated the osteogenicity process associated with bone constructs prepared from human Bone marrow-derived mesenchymal stem cells (hBMSC) ...
Manassero, Mathieu Viateau, Véronique Deschepper, Mickael Oudina, Karim Delphine Logeart-Avramoglou Hervé Petite Morad Bensidhoum
Published in
Tissue Engineering Part A
Tissue constructs containing mesenchymal stem cells (MSC) are an appealing strategy for repairing massive segmental bone defects. However, their therapeutic effectiveness does not match that of autologous bone grafts; among the complicating reasons, the scaffold resorbability has been identified as a critical feature for achieving bone regeneration...
de Peppo, Giuseppe Maria Sladkova, Martina Sjövall, Peter Palmquist, Anders Oudina, Karim Hyllner, Johan Thomsen, Peter Hervé Petite Karlsson, Camilla
Published in
Tissue Engineering Part A
Bone tissue engineering represents a promising strategy to obviate bone deficiencies, allowing the ex vivo construction of bone substitutes with unprecedented potential in the clinical practice. Considering that in the human body cells are constantly stimulated by chemical and mechanical stimuli, the use of bioreactor is emerging as an essential fa...
Becquart, Pierre Cambon-Binder, Adeline Monfoulet, Laurent-Emmanuel Bourguignon, Marianne Vandamme, Katleen Morad Bensidhoum Hervé Petite Delphine Logeart-Avramoglou
Published in
Tissue Engineering Part A
Local tissue ischemia is a prime cause responsible for the massive cell death in tissue-engineered (TE) constructs observed postimplantation. To assess the impact of ischemia on the death of implanted human multipotent stromal cells (hMSCs), which have great potential for repairing damaged tissues, we hereby investigated the in vivo temporal and sp...
Gui, Liqiong Zhao, Liping Spencer, Randal W Burghouwt, Arthur Taylor, M Scott Shalaby, Shalaby W Niklason, Laura E
Published in
Tissue Engineering Part A
Functional connective tissues have been developed using tissue engineering approach by seeding cells on biodegradable scaffolds such as polyglycolic acid (PGA). However, a major drawback of tissue engineering approaches that utilize synthetic polymers is the persistence of polymer remnants in engineered tissues at the end of culture. Such polymer f...
Qian, Yu Lin, Zhen Chen, Jimin Fan, Ying Davey, Tamara Cake, Martin Day, Robert Dai, Kerong Xu, Jiake Zheng, Minghao
...
Published in
Tissue Engineering Part A
Autologous bone graft, the standard of bone grafting in achieving spinal fusion, is associated with several limitations and complications. The use of bone marrow cells (BMCs) as a potential cell source for spinal fusion, combined with a suitable scaffold to promote bone formation, may be a better choice. The aims of this study were to evaluate the ...
