Mitchell, Joshua M June, Cynthia M Baggett, Vincent L Lowe, Beth C Ruble, James F Bonomo, Robert A Leonard, David A Powers, Rachel A
Published in
The Journal of biological chemistry
The evolution of multidrug resistance in Acinetobacter spp. increases the risk of our best antibiotics losing their efficacy. From a clinical perspective, the carbapenem-hydrolyzing class D β-lactamase subfamily present in Acinetobacter spp. is particularly concerning because of its ability to confer resistance to carbapenems. The kinetic profiles ...
Hernandez, Alfredo J Lee, Seung-Joo Thompson, Noah J Griffith, Jack D Richardson, Charles C
Published in
The Journal of biological chemistry
The T7 primase-helicase plays a pivotal role in the replication of T7 DNA. Using affinity isolation of peptide-nucleic acid crosslinks and mass spectrometry, we identify protein regions in the primase-helicase and T7 DNA polymerase that form contacts with the RNA primer and DNA template. The contacts between nucleic acids and the primase domain of ...
Li, Yang Huang, Shijia Wang, Jingsi Dai, Jianli Cai, Jie Yan, Shuai Huang, Zhiliang He, Shengqi Wang, Ping Liu, Jianmiao
...
Published in
The Journal of biological chemistry
Inositol-requiring enzyme 1 (IRE1) is an evolutionarily conserved sensor of endoplasmic reticulum (ER) stress and mediates a key branch of the unfolded protein response in eukaryotic cells. It is an ER-resident transmembrane protein that possesses Ser/Thr protein kinase and endoribonuclease (RNase) activities in its cytoplasmic region. IRE1 is acti...
Ghosh, Krishna Zhang, Guang-Fen Chen, Hong Chen, Shao-Rui Pan, Hui-Lin
Published in
The Journal of biological chemistry
Type-2 cannabinoid receptors (CB2, encoded by the Cnr2 gene) are mainly expressed in immune cells, and CB2 agonists normally have no analgesic effect. However, nerve injury upregulates CB2 in the dorsal root ganglion (DRG), following which CB2 stimulation reduces neuropathic pain. It is unclear how nerve injury increases CB2 expression or how CB2 a...
Puglielli, Luigi Ellis, Blake C Saunders, Aleister J Kovacs, Dora M
Published in
The Journal of biological chemistry
Farci, Domenica Haniewicz, Patrycja de Sanctis, Daniele Iesu, Luca Kereïche, Sami Winterhalter, Mathias Piano, Dario
Published in
The Journal of biological chemistry
The radiation-resistant bacterium Deinococcus radiodurans is known as the world's toughest bacterium. The S-layer of D. radiodurans, consisting of several proteins on the surface of the cellular envelope and intimately associated with the outer membrane, has therefore been useful as a model for structural and functional studies. Its main proteinace...
Oldach, Laurel
Published in
The Journal of biological chemistry
Yan, Kaizhou Stanley, Mathew Kowalski, Bartosz Raimi, Olawale G Ferenbach, Andrew T Wei, Pingzhen Fang, Wenxia van Aalten, Daan M F
Published in
The Journal of biological chemistry
Aspergillus fumigatus is the causative agent of invasive aspergillosis, an infection with mortality rates of up to 50%. The glucan-rich cell wall of A. fumigatus is a protective structure that is absent from human cells and is a potential target for antifungal treatments. Glucan is synthesized from the donor uridine diphosphate glucose, with the co...
Tsai, Wen-Hao Grauffel, Cédric Huang, Ming-Yueh Postić, Sandra Rupnik, Marjan Slak Lim, Carmay Yang, Shi-Bing
Published in
The Journal of biological chemistry
Opening of two-pore domain K+ channels (K2Ps) is regulated by various external cues, such as pH, membrane tension, or temperature, which allosterically modulate the selectivity filter (SF) gate. However, how these cues cause conformational changes in the SF of some K2P channels remains unclear. Herein, we investigate the mechanisms by which extrace...
Ma, Xue-Fei Wang, Ting-Ting Wang, Wen-Hui Guan, Li Guo, Chang-Run Li, Xing-Hua Lei, Yun-Tao Fan, Ying-Zhe Yang, Xiao-Na Hattori, Motoyuki
...
Published in
The Journal of biological chemistry
P2X receptors are a class of nonselective cation channels widely distributed in the immune and nervous systems, and their dysfunction is a significant cause of tumors, inflammation, leukemia, and immune diseases. P2X7 is a unique member of the P2X receptor family with many properties that differ from other subtypes in terms of primary sequence, the...
