Govind Kumar, Vivek Ogden, Dylan S Isu, Ugochi H Polasa, Adithya Losey, James Moradi, Mahmoud
Published in
The Journal of biological chemistry
Within the last two decades, severe acute respiratory syndrome coronaviruses 1 and 2 (SARS-CoV-1 and SARS-CoV-2) have caused two major outbreaks; yet for reasons not fully understood, the COVID-19 pandemic caused by SARS-CoV-2 has been significantly more widespread than the 2003 SARS epidemic caused by SARS-CoV-1 despite striking similarities betwe...
Castro, Manuel A Parson, Kristine F Beg, Ilyas Wilkinson, Mason C Nurmakova, Kamila Levesque, Iliana Voehler, Markus W Wolfe, Michael S Ruotolo, Brandon T Sanders, Charles R
...
Published in
The Journal of biological chemistry
This work reports substrate-selective inhibition of a protease with broad substrate specificity based on direct binding of a small-molecule inhibitor to the substrate. The target for these studies was γ-secretase protease, which cleaves dozens of different single-span membrane protein substrates, including both the C99 domain of the human amyloid p...
Kelly, Skyler L Szyjka, Courtney E Strobel, Eric J
Published in
The Journal of biological chemistry
Synchronized transcription elongation complexes (TECs) are a fundamental tool for in vitro studies of transcription and RNA folding. Transcription elongation can be synchronized by omitting one or more nucleoside triphosphates from an in vitro transcription reaction so that RNA polymerase can only transcribe to the first occurrence of the omitted n...
Zhang, Siyi Bai, Ping Lei, Dan Liang, Yingxia Zhen, Sherri Bakiasi, Grisilda Pang, Hao Choi, Se Hoon Wang, Changning Tanzi, Rudolph E
...
Published in
The Journal of biological chemistry
Epigenetic regulation plays substantial roles in human pathophysiology, which provides opportunities for intervention in human disorders through the targeting of epigenetic pathways. Recently, emerging evidence from preclinical studies suggested the potential in developing therapeutics of Alzheimer's disease (AD) by targeting bromodomain containing...
Wang, Zheng Lou, Jizhong Zhang, Hong
Published in
The Journal of biological chemistry
Intracellular spaces are partitioned into separate compartments to ensure that numerous biochemical reactions and cellular functions take place in a spatiotemporally controlled manner. Biomacromolecules including proteins and RNAs undergo liquid-liquid phase separation and subsequent phase transition to form biological condensates with diverse mate...
Shen, Gengyang Liu, Xin Lei, Wei Duan, Rong Yao, Zhenqiang
Published in
The Journal of biological chemistry
Osteoporosis is caused by enhanced bone resorption and relatively reduced bone formation. There is an unmet need to develop new agents with both antiresorptive and anabolic effects to treat osteoporosis, although drugs with either effect alone are available. A small molecular compound, plumbagin, was reported to inhibit receptor activator of nuclea...
Kelly, Rebecca L Huehls, Amelia M Venkatachalam, Annapoorna Huntoon, Catherine J Machida, Yuichi J Karnitz, Larry M
Published in
The Journal of biological chemistry
Replication stress impedes DNA polymerase progression causing activation of the ataxia telangiectasia and Rad3-related signaling pathway, which promotes the intra-S phase checkpoint activity through phosphorylation of checkpoint kinase 1 (Chk1). Chk1 suppresses replication origin firing, in part, by disrupting the interaction between the preinitiat...
Dutta, Soumajit Selvam, Balaji Das, Aditi Shukla, Diwakar
Published in
The Journal of biological chemistry
Cannabinoid receptor 1 (CB1) is a therapeutically relevant drug target for controlling pain, obesity, and other central nervous system disorders. However, full agonists and antagonists of CB1 have been reported to cause serious side effects in patients. Therefore, partial agonists have emerged as a viable alternative as they can mitigate overstimul...
Wear, Maggie P Jacobs, Ella Wang, Siqing McConnell, Scott A Bowen, Anthony Strother, Camilla Cordero, Radames J B Crawford, Conor J Casadevall, Arturo
Published in
The Journal of biological chemistry
The polysaccharide capsule of fungal pathogen Cryptococcus neoformans is a critical virulence factor that has historically evaded complete characterization. Cryptococcal polysaccharides are known to either remain attached to the cell as capsular polysaccharides (CPSs) or to be shed into the extracellular space as exopolysaccharides (EPSs). While ma...
Sarker, Animesh Rathore, Abhishek Singh Khalid, Md Fahim Gupta, Rinkoo Devi
Published in
The Journal of biological chemistry
Dengue is one of the most dominant arthropod-borne viral diseases, infecting at least 390 million people every year throughout the world. Despite this, there is no effective treatment against dengue, and the only available vaccine has already been withdrawn owing to the significant adverse effects. Therefore, passive immunotherapy using monoclonal ...
