Dünkler, Alexander Leda, Marcin Kromer, Jan-Michael Neller, Joachim Gronemeyer, Thomas Goryachev, Andrew B. Johnsson, Nils
Published in
The Journal of Cell Biology
The polarisome is a cortical proteinaceous microcompartment that organizes the growth of actin filaments and the fusion of secretory vesicles in yeasts and filamentous fungi. Polarisomes are compact, spotlike structures at the growing tips of their respective cells. The molecular forces that control the form and size of this microcompartment are no...
Murillo-Pineda, Marina Valente, Luis P Dumont, Marie Mata, João F Fachinetti, Daniele Jansen, Lars E T
Published in
The Journal of cell biology
Human centromeres form primarily on α-satellite DNA but sporadically arise de novo at naive ectopic loci, creating neocentromeres. Centromere inheritance is driven primarily by chromatin containing the histone H3 variant CENP-A. Here, we report a chromosome engineering system for neocentromere formation in human cells and characterize the first exp...
Fic, Weronika Bastock, Rebecca Raimondi, Francesco Los, Erinn Inoue, Yoshiko Gallop, Jennifer L. Russell, Robert B. St Johnston, Daniel
Published in
The Journal of Cell Biology
Cdc42-GTP is required for apical domain formation in epithelial cells, where it recruits and activates the Par-6–aPKC polarity complex, but how the activity of Cdc42 itself is restricted apically is unclear. We used sequence analysis and 3D structural modeling to determine which Drosophila GTPase-activating proteins (GAPs) are likely to interact wi...
Rabanal-Ruiz, Yoana Byron, Adam Wirth, Alexander Madsen, Ralitsa Sedlackova, Lucia Hewitt, Graeme Nelson, Glyn Stingele, Julian Wills, Jimi C. Zhang, Tong
...
Published in
The Journal of Cell Biology
The mammalian target of rapamycin complex 1 (mTORC1) integrates mitogenic and stress signals to control growth and metabolism. Activation of mTORC1 by amino acids and growth factors involves recruitment of the complex to the lysosomal membrane and is further supported by lysosome distribution to the cell periphery. Here, we show that translocation ...
Schuck, Sebastian Prinz, William A. Thorn, Kurt S. Voss, Christiane Walter, Peter
Published in
The Journal of Cell Biology
Clancy, Anne Heride, Claire Pinto-Fernández, Adán Elcocks, Hannah Kallinos, Andreas Kayser-Bricker, Katherine J. Wang, Weiping Smith, Victoria Davis, Simon Fessler, Shawn
...
Published in
The Journal of Cell Biology
Ayukawa, Rie Iwata, Seigo Imai, Hiroshi Kamimura, Shinji Hayashi, Masahito Ngo, Kien Xuan Minoura, Itsushi Uchimura, Seiichi Makino, Tsukasa Shirouzu, Mikako
...
Published in
The Journal of Cell Biology
Nucleation of microtubules (MTs) is essential for cellular activities, but its mechanism is unknown because of the difficulty involved in capturing rare stochastic events in the early stage of polymerization. Here, combining rapid flush negative stain electron microscopy (EM) and kinetic analysis, we demonstrate that the formation of straight oligo...
Tian, Yuan Wei, Chenxi He, Jianfeng Yan, Yuxuan Pang, Nan Fang, Xiaomin Liang, Xin Fu, Jingyan
Published in
The Journal of Cell Biology
The centrosome is the main microtubule-organizing center in animal cells. It comprises of two centrioles and the surrounding pericentriolar material. Protein organization at the outer layer of the centriole and outward has been studied extensively; however, an overall picture of the protein architecture at the centriole core has been missing. Here ...
Watson, Joseph L Aich, Samya Oller-Salvia, Benjamí Drabek, Andrew A Blacklow, Stephen C Chin, Jason Derivery, Emmanuel
Published in
The Journal of cell biology
Protein micropatterning allows proteins to be precisely deposited onto a substrate of choice and is now routinely used in cell biology and in vitro reconstitution. However, drawbacks of current technology are that micropatterning efficiency can be variable between proteins and that proteins may lose activity on the micropatterns. Here, we describe ...
Clancy, Anne Heride, Claire Pinto-Fernández, Adán Elcocks, Hannah Kallinos, Andreas Kayser-Bricker, Katherine J. Wang, Weiping Smith, Victoria Davis, Simon Fessler, Shawn
...
Published in
The Journal of Cell Biology
When a ribosome stalls during translation, it runs the risk of collision with a trailing ribosome. Such an encounter leads to the formation of a stable di-ribosome complex, which needs to be resolved by a dedicated machinery. The initial stalling and the subsequent resolution of di-ribosomal complexes requires activity of Makorin and ZNF598 ubiquit...