Bokhobza Alexandre Gkika, Dimitra Lemonnier, Loic Shapovalov, George Gordienko, Dmitri Poux, Céline Bernardini, Michela Bokhobza, Alexandre Bidaux, Gabriel Degerny, Cindy
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Published in
The Journal of Cell Biology
TRPM8 is a cold sensor that is highly expressed in the prostate as well as in other non-temperature-sensing organs, and is regulated by downstream receptor-activated signaling pathways. However, little is known about the intracellular proteins necessary for channel function. Here, we identify two previously unknown proteins, which we have named "TR...
Published in
Neuron
Summary Neurofibrillary tangles advance from layer II of the entorhinal cortex (EC-II) toward limbic and association cortices as Alzheimer's disease evolves. However, the mechanism involved in this hierarchical pattern of disease progression is unknown. We describe a transgenic mouse model in which overexpression of human tau P301L is restricted to...
Hong, Soonjin Troyanovsky, Regina B Troyanovsky, Sergey M
Published in
The Journal of cell biology
The cadherin extracellular region produces intercellular adhesion clusters through trans- and cis-intercadherin bonds, and the intracellular region connects these clusters to the cytoskeleton. To elucidate the interdependence of these binding events, cadherin adhesion was reconstructed from the minimal number of structural elements. F-actin-uncoupl...
Ngok, Siu P Geyer, Rory Liu, Miaoliang Kourtidis, Antonis Agrawal, Sudesh Wu, Chuanshen Seerapu, Himabindu Reddy Lewis-Tuffin, Laura J Moodie, Karen L Huveldt, Deborah
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Published in
The Journal of cell biology
Vascular endothelial growth factor (VEGF) and Ang1 (Angiopoietin-1) have opposing effects on vascular permeability, but the molecular basis of these effects is not fully known. We report in this paper that VEGF and Ang1 regulate endothelial cell (EC) junctions by determining the localization of the RhoA-specific guanine nucleotide exchange factor S...
Published in
The Journal of Cell Biology
By phosphorylating Thr3 of histone H3, Haspin promotes centromeric recruitment of the chromosome passenger complex (CPC) during mitosis. Aurora B kinase, a CPC subunit, sustains chromosome bi-orientation and the spindle assembly checkpoint (SAC). Here, we characterize the small molecule 5-iodotubercidin (5-ITu) as a potent Haspin inhibitor. In vitr...
Wang, Fangwei Ulyanova, Natalia P Daum, John R Patnaik, Debasis Kateneva, Anna V Gorbsky, Gary J Higgins, Jonathan M G
Published in
The Journal of cell biology
Haspin phosphorylates histone H3 at threonine-3 (H3T3ph), providing a docking site for the Aurora B complex at centromeres. Aurora B functions to correct improper kinetochore-microtubule attachments and alert the spindle checkpoint to the presence of misaligned chromosomes. We show that Haspin inhibitors decreased H3T3ph, resulting in loss of centr...
Yoon, Mee-Sup Du, Guangwei Backer, Jonathan M Frohman, Michael A Chen, Jie
Published in
The Journal of cell biology
The rapamycin-sensitive mammalian target of rapamycin (mTOR) complex, mTORC1, regulates cell growth in response to mitogenic signals and amino acid availability. Phospholipase D (PLD) and its product, phosphatidic acid, have been established as mediators of mitogenic activation of mTORC1. In this study, we identify a novel role for PLD1 in an amino...
Petsalaki, Eleni Akoumianaki, Tonia Black, Elizabeth J Gillespie, David A F Zachos, George
Published in
The Journal of cell biology
Aurora B kinase activity is required for successful cell division. In this paper, we show that Aurora B is phosphorylated at serine 331 (Ser331) during mitosis and that phosphorylated Aurora B localizes to kinetochores in prometaphase cells. Chk1 kinase is essential for Ser331 phosphorylation during unperturbed prometaphase or during spindle disrup...
Brill, Monika S Lichtman, Jeff W Thompson, Wesley Zuo, Yi Misgeld, Thomas
Published in
The Journal of cell biology
Schwann cells (SCs), the glial cells of the peripheral nervous system, cover synaptic terminals, allowing them to monitor and modulate neurotransmission. Disruption of glial coverage leads to axon degeneration and synapse loss. The cellular mechanisms that establish and maintain this coverage remain largely unknown. To address this, we labeled sing...
Rohn, Jennifer L Sims, David Liu, Tao Fedorova, Marina Schöck, Frieder Dopie, Joseph Vartiainen, Maria K Kiger, Amy A Perrimon, Norbert Baum, Buzz
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Published in
The Journal of cell biology
Although a large number of actin-binding proteins and their regulators have been identified through classical approaches, gaps in our knowledge remain. Here, we used genome-wide RNA interference as a systematic method to define metazoan actin regulators based on visual phenotype. Using comparative screens in cultured Drosophila and human cells, we ...
