Published in The EMBO Journal
The phosphoinositides (PIs) function as efficient and finely tuned switches that control the assembly–disassembly cycles of complex molecular machineries with key roles in membrane trafficking. This important role of the PIs is mainly due to their versatile nature, which is in turn determined by their fast metabolic interconversions. PIs can be tig...
Published in The EMBO Journal
Published in The EMBO journal
Published in The EMBO Journal
The proper localization of RNA transcripts is a highly controlled and fine‐tuned process. Indeed, regulation of RNA trafficking is mediated by both cis ‐acting elements and trans ‐acting factors, and defects in either mechanism have been associated with disease. Identifying the RNA sequence motifs that determine cellular localization for a given tr...
Published in The EMBO journal
Published in The EMBO Journal
Canonically, LC 3 lipidation has been associated with autophagy pathways but it becomes increasingly clear that this modification can also occur during autophagy‐unrelated processes. In this issue, Florey and colleagues find that the WD 40 domain of ATG 16L1 is dispensable for LC 3 lipidation during starvation‐induced autophagy but required for its...
Published in The EMBO journal
Growth differentiation factor 8 (GDF8)/myostatin is a latent TGF-β family member that potently inhibits skeletal muscle growth. Here, we compared the conformation and dynamics of precursor, latent, and Tolloid-cleaved GDF8 pro-complexes to understand structural mechanisms underlying latency and activation of GDF8. Negative stain electron microscopy...
Published in The EMBO journal
Host cell defense against an invading pathogen depends upon various multifactorial mechanisms, several of which remain undiscovered. Here, we report a novel defense mechanism against mycobacterial infection that utilizes the histone methyltransferase, SUV39H1. Normally, a part of the host chromatin, SUV39H1, was also found to be associated with the...
Published in The EMBO Journal
An account of how a surprising finding became a controversial topic, and how a group of researchers persevered over 20 years to attain broad acceptance.
Published in The EMBO Journal
Myostatin, a key regulator of muscle mass in vertebrates, is biosynthesised as a latent precursor in muscle and is activated by sequential proteolysis of the pro‐domain. To investigate the molecular mechanism by which pro‐myostatin remains latent, we have determined the structure of unprocessed pro‐myostatin and analysed the properties of the prote...
