Watanabe, Momoko Buth, Jessie E Haney, Jillian R Vishlaghi, Neda Turcios, Felix Elahi, Lubayna S Gu, Wen Pearson, Caroline A Kurdian, Arinnae Baliaouri, Natella V
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Published in
Stem cell reports
Telencephalic organoids generated from human pluripotent stem cells (hPSCs) are a promising system for studying the distinct features of the developing human brain and the underlying causes of many neurological disorders. While organoid technology is steadily advancing, many challenges remain, including potential batch-to-batch and cell-line-to-cel...
Tang, Qingyuan Schweitzer, Jeffrey S. Song, Bin
Published in
Stem Cell Reports
In a recently published study, Xu et al. used two surface markers, CLSTN2 and PTPRO , to generate highly purified donor dopaminergic neurons and achieved stable and predictable therapeutic outcomes by transplantation into the brain of PD animal models (Xu et al., 2022).
Yadav, Sudhanshu Garrido, Antonio Hernández, M. Carmen Oliveros, Juan C. Pérez-García, Vicente Fraga, Mario F. Carrera, Ana C.
Published in
Stem Cell Reports
Yadav et al. report a pathway dismantling the repression imposed by the EZH2 repressor on the NODAL promoter, (required for stemness), and on the promoters of genes needed for primitive streak formation. A kinase-independent action of PI3Kβ is required for this action as it triggers activation of a pathway involving a cRAC1/JNK-mediated efficient a...
Vuidel, Aurore Cousin, Loïc Weykopf, Beatrice Haupt, Simone Hanifehlou, Zahra Wiest-Daesslé, Nicolas Segschneider, Michaela Lee, Joohyun Kwon, Yong-Jun Peitz, Michael
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Published in
Stem Cell Reports
In this article, Wilbertz and colleagues show that machine learning based on phenotypic features can distinguish Parkinson’s disease patient stem cell-derived dopaminergic neurons from isogenic controls. LRRK2 G2019S or SNCA triplication neurons were fluorescently stained, and quantitative phenotypic features were extracted. The resulting phenotypi...
Drouin-Ouellet, Janelle Legault, Emilie M. Nilsson, Fredrik Pircs, Karolina Bouquety, Julie Petit, Florence Shrigley, Shelby Birtele, Marcella Pereira, Maria Storm, Petter
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Published in
Stem Cell Reports
Drouin-Ouellet and colleagues have employed an efficient method to generate induced dopaminergic neurons derived from PD patients. These cells (unlike iPSC-derived dopaminergic neurons from these same patients) show age-related autophagy impairments that lead to an accumulation of phosphorylated alpha-synuclein. As such, this study presents a uniqu...
Xu, Ting Duan, Jinjin Li, Yingqi Wang, Guanhao Li, Shuanqing Li, You Lu, Wenting Yan, Xinyi Ren, Yixuan Guo, Fei
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Published in
Stem Cell Reports
Dr. Lu and colleagues generate a TPH2-EGFP reporter system for human serotonin neurons (SNs). The reporter system enables scientists to detect electrophysiological features of SNs and to obtain purified SNs for transcriptional analysis and the study of pharmacological responses to antidepressants. This reporter system provides a versatile tool for ...
Tower, Robert J. Bancroft, Alec C. Chowdary, Ashish R. Barnes, Spencer Edwards, Nicole J. Pagani, Chase A. Dawson, Lindsay A. Levi, Benjamin
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Stem Cell Reports
Tower et al. combine computational and histological approaches to explore the healing process after regenerative and fibrotic musculoskeletal injuries. This article points to transcriptional profiles correlating with healing outcome more than injury type or cell source and indicates unique inflammatory pathways activated in the fibroblast-like mese...
Yang, Chengyu Li, Zeshun Liu, Yang Hou, Runpeng Lin, Minmin Fu, Linhao Wu, Decheng Liu, Quanying Li, Kai Liu, Chao
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Published in
Stem Cell Reports
Mesenchymal stromal cell (MSC) transplantation could enhance bone repair, but their cell fate is poorly understood. In this article, Liu and colleagues utilize a single-cell 3D spatial correlation (sc3DSC) method to reveal that a large portion of transplanted MSCs maintain stemness, localize around blood vessels, increase blood vessel formation, an...
Zhao, Xin Shen, Jian Zhao, Xuan Zhang, Miao Feng, Xiao Zhang, Weiyu Lu, Xinyi
Published in
Stem Cell Reports
Zhao et al. discover the PIM3 signaling pathway as a key route to repress MuERVL and 2-cell-like totipotent state in ESCs. Pim3 loss allows phosphorylation of AMPK, which phosphorylates HDAC4/5 and promotes their transport out of the nucleus, thereby permitting acetylation of H3K9 and interfering with H3K9me1/2 deposition on MuERVL. Their findings ...
Pré, Deborah Wooten, Alexander T Biesmans, Steven Hinckley, Sandy Zhou, Haowen Sherman, Sean P Kakad, Priyanka Gearhart, Jeffrey Bang, Anne G
Published in
Stem cell reports
Impairment of long-term potentiation (LTP) is a common feature of many pre-clinical models of neurological disorders; however, studies in humans are limited by the inaccessibility of the brain. Human induced pluripotent stem cells (hiPSCs) provide a unique opportunity to study LTP in disease-specific genetic backgrounds. Here we describe a multi-el...
