Krasemann, Susanne Haferkamp, Undine Pfefferle, Susanne Woo, Marcel S. Heinrich, Fabian Schweizer, Michaela Appelt-Menzel, Antje Cubukova, Alevtina Barenberg, Janica Leu, Jennifer
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Published in
Stem Cell Reports
In this article, Pless and colleagues show upregulation of IFNγ signaling in the neurovascular unit of the brain in fatal COVID-19. They show that an hiPSC-derived brain capillary endothelial cell model can be infected with SARS-CoV-2, resulting in similar expression changes, viral replication, and release while endothelial cell integrity is mainta...
Kitagawa, Takahiro Nagoshi, Narihito Kamata, Yasuhiro Kawai, Momotaro Ago, Kentaro Kajikawa, Keita Shibata, Reo Sato, Yuta Imaizumi, Kent Shindo, Tomoko
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Published in
Stem Cell Reports
This study by Okano, Nagoshi, and colleagues demonstrated decreased locomotor function in hiPSC-NS/PC-transplanted SCI mice induced by inhibition of graft-derived neuronal activity using a DREADD. Locomotor changes caused by the graft neuronal activity inhibition were observed exclusively in the chronic phase. These results suggested the significan...
Arjona, Marina Goshayeshi, Armon Rodriguez-Mateo, Cristina Brett, Jamie O. Both, Pieter Ishak, Heather Rando, Thomas A.
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Stem Cell Reports
In this article, Arjona et al. show that TubA, an HDAC6 inhibitor, can inhibit primary cilium resorption and preserve MuSC quiescence and stem cell potency ex vivo . Indeed, treatment with TubA maintains MuSC engraftment potential and induces a return to quiescence in cycling MuSCs, revealing a potentially valuable approach to enhancing the therape...
George, Aman Sharma, Ruchi Pfister, Tyler Abu-Asab, Mones Hotaling, Nathan Bose, Devika DeYoung, Charles Chang, Justin Adams, David R. Cogliati, Tiziana
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Published in
Stem Cell Reports
In this article, Brooks and colleagues establish an iPSC-derived retinal pigment epithelium (iRPE) model of two forms of oculocutaneous albinism, OCA1A and OCA2. Both OCA-iRPE monolayers are morphologically normal, albeit with differences in cellular phenotypes. Pigmentation and melanosome content are abnormal. OCA-iRPE models will help with dissec...
Sharma, Arun Clemens, Rachel A. Garcia, Orquidea Taylor, D. Lansing Wagner, Nicole L. Shepard, Kelly A. Gupta, Anjali Malany, Siobhan Grodzinsky, Alan J. Kearns-Jonker, Mary
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Published in
Stem Cell Reports
Sharma and colleagues recap the 2020 Biomanufacturing in Space Symposium, which reviewed space-based regenerative medicine research and discussed opportunities to leverage low Earth orbit (LEO) to advance biomanufacturing for regenerative medicine. This perspective will highlight key biomanufacturing opportunities in LEO, note current technical gap...
Hulme, Amy J. Maksour, Simon St-Clair Glover, Mitchell Miellet, Sara Dottori, Mirella
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Stem Cell Reports
Forced expression of the transcription factor NGN2 has been widely used to directly convert hPCSs, neural progenitors, or fibroblasts to functionally mature neurons of the central and peripheral nervous system (PNS). The article by Dottori and colleagues summarizes the cellular roles of NGN2 and describes the applications and limitations of using N...
Aldrin-Kirk, Patrick Åkerblom, Malin Cardoso, Tiago Nolbrant, Sara Adler, Andrew F. Liu, Xiaohe Heuer, Andreas Davidsson, Marcus Parmar, Malin Björklund, Tomas
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Published in
Stem Cell Reports
Aldrin-Kirk and colleagues report a novel method developed to investigate specific afferent connections to human embryonic stem cell (hESC)-derived dopamine (DA) grafts from distinct anatomical projections in the recipient brain. Using a novel retrograde adeno-associted virus capsid (MNM008) together with monosynaptic rabies-based tracing, they dem...
Vojtek, Matúš Zhang, Jingchao Sun, Juanjuan Zhang, Man Chambers, Ian
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Stem Cell Reports
Vojtek and colleagues show that NANOG represses Otx2 to induce cytokine-free specification of primordial germ cell-like cells (PGCLCs). OTX2 levels are critical for PGCLC induction, as sustained OTX2 expression prevents NANOG-induced differentiation. Although ESRRB can functionally substitute for NANOG in other processes, ESRRB does not repress Otx...
Autar, Kaveena Guo, Xiufang Rumsey, John W. Long, Christopher J. Akanda, Nesar Jackson, Max Narasimhan, Narasimhan S. Caneus, Julbert Morgan, Dave Hickman, James J.
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Published in
Stem Cell Reports
In this article, Hickman and colleagues used controlled hiPSC-cortical neuron maturation to develop models capable of evaluating functional deficits in long-term potentiation, which is the basis for many neurodegenerative diseases. This model has the potential for patient-specific personalized medicine and can be used for both the investigation of ...
Roig-Merino, Alicia Urban, Manuela Bozza, Matthias Peterson, Julia D. Bullen, Louise Büchler-Schäff, Marleen Stäble, Sina van der Hoeven, Franciscus Müller-Decker, Karin McKay, Tristan R.
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Published in
Stem Cell Reports
In this study, Harbottle and colleagues show that nonviral, nonintegrating, and autonomously replicating minimally sized DNA nanovectors can be used to persistently modify SCs genetically without causing any molecular or genetic damage. The DNA vectors remain episomal during self-renewal and differentiation in vitro and in vivo , while providing ro...
