Published in Stem Cell Reports
Brivanlou and colleagues describe a high-throughput platform to generate self-organizing human lung buds from hESCs on microchips. Lung buds display in vivo -like proximo-distal patterning of lung tissue, and are amenable to high-throughput quantitative analysis of SARS-CoV-2 cell type-specific infection, transmission, and cytopathology. Transcript...
Published in Stem cell reports
The thymus is critical for the establishment of a functional and self-tolerant adaptive immune system but involutes with age, resulting in reduced naive T cell output. Generation of a functional human thymus from human pluripotent stem cells (hPSCs) is an attractive regenerative strategy. Direct differentiation of thymic epithelial progenitors (TEP...
Published in Stem Cell Reports
Published in Stem Cell Reports
Zhao and colleagues screen out and assess the effects of Lovastatin on the self-renewal of mouse spermatogonial stem cells (mSSCs) by inhibiting its inflammation and apoptosis through the regulation of isoprenoid intermediates. Lovastatin could promote the self-renewal of both murine and primate SSCs and have implications for the treatment of certa...
Published in Stem cell reports
Chromosome 15q11-q13 duplication syndrome (Dup15q) is a neurodevelopmental disorder caused by maternal duplications of this region. Autism and epilepsy are key features of Dup15q. UBE3A, which encodes an E3 ubiquitin ligase, is likely a major driver of Dup15q because UBE3A is the only imprinted gene expressed solely from the maternal allele. Nevert...
Published in Stem Cell Reports
Singeç and colleagues (Deng et al.) present a robust and reproducible sensory neuron differentiation method for human pluripotent stem cells that enable translational studies. Generation of pseudo-unipolar sensory neurons is scalable with a robotic platform and extensive molecular and functional characterization and comparison to DRG neurons and ot...
Published in Stem Cell Reports
In this article, Ravn and colleagues report on a first public dialogue on organoid technologies with a diverse group of stakeholders to identify their perceptions and concerns. Participants generally support organoid research on the condition that responsible governance, ethical oversight, and sound informed consent procedures are in place. Yet, a ...
Published in Stem Cell Reports
The corresponding author and colleagues demonstrated that epicardially secreted fibronectin augmented cardiomyocyte maturation in 3D-engineered heart tissues and provided a genetic map of the key regulatory pathways governing epicardial-myocardial crosstalk. As the epicardium is crucial for cardiac development and cardiomyocyte maturation, these fi...
Published in Stem Cell Reports
In this article, Fu and colleagues show that WNT7A decreases adipogenesis of skeletal muscle-derived fibro-adipogenic progenitors. WNT7A promotes nuclear localization and retention of YAP/TAZ in a β-CATENIN-independent manner in differentiating fibro-adipogenic progenitors. When administered intramuscularly in vivo following glycerol-induced injury...
Published in Stem Cell Reports
In this report, Keshet and colleagues explore the genomic imprinting effect on ovarian development by differentiating biparental, parthenogenetic, and androgenetic human pluripotent stem cells into granulosa-like cells. They show that parthenogenetic cells display a reduced differentiation capacity, due to lack of expression of the paternal gene IG...
