Ariu, F Bogliolo, L Pinna, A Malfatti, L Innocenzi, P Falchi, L Bebbere, D Ledda, S
Published in
Reproduction, fertility, and development
The present study investigated whether supplementation with different doses of cerium dioxide nanoparticles (CeO2 NPs) during in vitro maturation (IVM) of prepubertal ovine oocytes influenced their embryonic development in vitro. Cumulus-oocyte complexes derived from the ovaries of slaughtered prepubertal sheep underwent IVM with CeO2NPs (0, 44, 88...
Mruk, Dolores D Bonanomi, Michele Silvestrini, Bruno
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Reproduction, fertility, and development
Several compounds affect male fertility by disrupting the adhesion of germ cells to Sertoli cells, which results in the release of undeveloped germ cells into the seminiferous tubule lumen that are incapable of fertilising the ovum. Indazole carboxylic acids are one class of compounds exhibiting such effects and they have been investigated as non-h...
Rutigliano, Heloisa M Wilhelm, Amanda Hall, Justin Shi, Bi Meng, Qinggang Stott, Rusty Bunch, Thomas D White, Kenneth L Davies, Christopher J Polejaeva, Irina A
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Reproduction, fertility, and development
The present retrospective study investigated pregnancy rates, the incidence of pregnancy loss and large offspring syndrome (LOS) and immune-related gene expression of sheep and goat somatic cell nuclear transfer (SCNT) pregnancies. We hypothesised that significantly higher pregnancy losses observed in sheep compared with goat SCNT pregnancies are d...
Ferreira, L M Garcia-Herreros, M Domingos, A Marques, C C Mesquita, P Barbas, J P Baptista, M C Pimenta, J Horta, A E M Prates, J A M
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Reproduction, fertility, and development
The aim of the present study was to examine the role of Doppel protein in the capacitation process and fertilising ability of both fresh and frozen-thawed (FT) spermatozoa from rams carrying different prion protein 2 (dublet) (PRND) gene polymorphisms. The detection efficacy of new anti-Doppel monoclonal antibodies and PRND mRNA quantification were...
Ferré, Luis B Bogliotti, Yanina Chitwood, James L Fresno, Cristóbal Ortega, Hugo H Kjelland, Michael E Ross, Pablo J
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Reproduction, fertility, and development
The aim of the present study was to evaluate the effects of sperm motility enhancers and different IVF times on cleavage, polyspermy, blastocyst formation, embryo quality and hatching ability. In Experiment 1, sex-sorted X chromosome-bearing Bos taurus spermatozoa were incubated for 30min before 18h fertilisation with hyperactivating factors, namel...
Takeo, Shun Kimura, Koji Shirasuna, Koumei Kuwayama, Takehito Iwata, Hisataka
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Reproduction, fertility, and development
Maternal age affects the quality of oocytes. The present study examined whether follicular fluid (FF) is a casual factor for age-associated decline in oocyte quality. First, we measured the concentration of advanced glycation end-products (AGE) in FF derived from young (21-45 months; Young-FF) and aged (≥120 months; Aged-FF) cows and found signific...
Tehrani, F Ramezani Zarkesh, M Tohidi, M Azizi, F Zadeh-Vakili, A
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Reproduction, fertility, and development
Polycystic ovary syndrome (PCOS) is frequently accompanied by insulin resistance (IR). The aim of the present study was to investigate whether the genetic association between insulin resistance and two single nucleotide polymorphisms (SNPs), namely rs7903146 (C/T) in transcription factor 7-like 2 (TCF7L2) and rs1111875 (A/G) in haematopoietically e...
Gerger, R P C Zago, F C Ribeiro, E S Gaudencio Neto, S Martins, L T Aguiar, L H Rodrigues, V H V Furlan, F H Ortigari, I Sainz, R D
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Reproduction, fertility, and development
Cloning procedures often interfere with conceptus growth and life ex utero, in a set of symptoms known as abnormal offspring syndrome (AOS). The aim of the present study was to compare the developmental pattern of in vivo-derived (IVD), IVF-derived and handmade cloning-derived (NT-HMC) Day 225 bovine concepti using established procedures. Pregnancy...
Praxedes, Érica C G Lima, Gabriela L Silva, Andréia M Apolinário, Carlos A C Bezerra, José A B Souza, Ana L P Oliveira, Moacir F Rodrigues, Ana P R Silva, Alexandre R
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Reproduction, fertility, and development
The aim of the present study was to characterise the ovarian preantral follicle (PF) population and to establish a solid surface vitrification (SSV) process using dimethyl sulfoxide (DMSO) as a cryoprotectant for preservation of ovarian tissue from yellow-toothed cavies (Galea spixii). Ovaries were fixed for PF population analysis or were subjected...
Arnold, Daniel R Gaspar, Roberta C da Rocha, Carlos V Sangalli, Juliano R de Bem, Tiago H C Corrêa, Carolina A P Penteado, João C T Meirelles, Flavio V Lopes, Flavia L
Published in
Reproduction, fertility, and development
Abnormal placental development is frequent in nuclear transfer (NT) pregnancies and is likely to be associated with altered epigenetic reprogramming. In the present study, fetal and placental measurements were taken on Day 60 of gestation in cows with pregnancies produced by AI, IVF and NT. Placentas were collected and subjected to histological eva...
