Blackmore, R V Linn, S
Published in
Nucleic acids research
Four hitherto undescribed endodeoxyribonucleases, temporarily designated A(1), A(2), A(3), and B, have been isolated from E. coli K-12. Each requires Mg(++) and is not stimulated by ATP or S-adenosylmethionine. A(3) is strongly inhibited by Fe(+++) and weakly inhibited by ATP, S-adenosylmethionine, and DPN, whereas B is inhibited by caffeine. Each ...
Feunteun, J Rosset, R Ehresmann, C Stiegler, P Fellner, P
Published in
Nucleic acids research
The precursor and mature 16S ribosomal RNAs from a novel thermosensitive ribosomal assembly defective mutant of E. coli, in which genetic evidence suggests that the ribosomal protein S4 is altered, have been isolated and characterised by finger-printing methods. The precursor 16S RNA, which is accumulated at 42 degrees , appears to be identical wit...
Kitamura, N Hayatsu, H
Published in
Nucleic acids research
When a solution containing 2 mM uridine, 20 mM sodium bisulfite, 0.1 mM MnCl(2), and 100 mM sodium phosphate buffer of pH 7.0 was incubated aerobically at 37 degrees or 0 degrees , partial cleavage of the glycosidic linkage of uridine took place. About 20% of the uridine was converted to uracil by the incubation for 4 hrs. Cytosine was produced fro...
Burdon, R H Douglas, J T
Published in
Nucleic acids research
Soluble factors appear to be present in both nuclei and cytoplasm of Krebs 2 ascites tumour cells capable of stimulating the enzyme catalysed methylation of DNA in isolated nuclei from these cells.
Feunteun, J Monier, R Vola, C Rosset, R
Published in
Nucleic acids research
Ikehara, M Fukui, T Koide, T Inaba, J
Published in
Nucleic acids research
Poly 7-deazainosinic acid has been prepared by the deamination and phosphorylation of tubercidin and the nucleoside diphosphate was polymerised using polynucleotide phosphorylase. The polymer has similar physical properties to poly(I), but has a low thermal stability in the double-stranded complex with poly(C). Poly(7-deaza I), in contrast, forms a...
Müller, W E Obermeier, J Totsuka, A Zahn, R K
Published in
Nucleic acids research
The agents daunomycin, ethidium bromide, distamycin A and cytochrome c inhibit DNA dependent DNA polymerase I (E. coli) reaction competitively to DNA. The influence of these template inactivators on the binding of DNA polymerase to native as well as denatured DNA has been determined by affinity chromatography. Cytochrome c blocks the binding of the...
Piper, P W Clark, B F
Published in
Nucleic acids research
Methoxyamine reacts selectively with tRNA molecules at certain exposed cytosine residues usually located in non base-paired regions of the two dimensional clover leaf structure. Here methoxyamine is used for the first time in a study of a mammalian tRNA structure. One of the sequence abnormalities of myeloma initiator tRNA is a cytosine instead of ...
Wilhelm, F X de Murcia, G M Daune, M P
Published in
Nucleic acids research
In native nucleoprotein, the premelting structural changes of DNA are not observed by circular dichroism measurements. In order to determine which protein fraction of chromatin is responsible for the absence of premelting we have examined a series of nucleoproteins depleted of different protein fractions by treatment with sodium chloride or sodium ...
Szeto, K S Söll, D
Published in
Nucleic acids research
We describe a method for obtaining radioactive fingerprints from nonradioactive ribonucleic acid. Fragments derived by T1 ribonuclease digestion of RNA are dephosphorylated with bacterial alkaline phosphatase. When these fragments are used as primers for the reaction of primer dependent polynucleotide phosphorylase with [alpha-(32)P]GDP in the pres...
