Published in Neurochemical research
Mitochondria and nuclei of various tissues, including brain and liver, are capable of producingγ-aminobutyrate (GABA) fromL-glutamate, but poorly, if at all, fromD-glutamate. The amino nitrogen of glutamate is found in the reaction product. The enzymes responsible for GABA formation were solubilized from crude liver cell nuclei by Triton X-100. The...
Published in Neurochemical research
A single injection of fenfluramine orp-chloroamphetamine (PCA) (100 μmol/kg intraperitoneally) decreases the serotonin (5HT) content and the tryptophan hydroxylase activity in various areas of the rat brain. Other reports have shown that a single injection of fenfluramine or PCA causes cytopathological changes in a serotonergic midbrain nucleus whi...
Published in Neurochemical research
The effect of methionine on the uptake, distribution, and binding of the convulsant methionine sulfoximine (MSO) in 7 rat brain regions, the spinal cord, the liver, and the kidney was investigated. The administration of methionine decreased the uptake of MSO in all brain regions. The uptake of MSO by and its distribution in the nervous tissue was u...
Published in Neurochemical research
Changes in protein components of purified myelin were measured following incubation in vitro with purified intra- and extracellular enzymes. Incubation with calf brain cathepsin D did not result in a significant relese of acid-soluble peptides as measured by ninhydrin analysis but was accompanied by a large loss of myelin proteins as determined on ...
Published in Neurochemical research
Brain glutamate decraboxylase (EC 4.1.1.15) catalyzes the biosynthesis of the postulated neurotransmitterγ-aminobutyric acid according to the following chemical equation:L-glutamate →γ-aminobutyric acid+CO2. Hydroxylamine treatment of the decarboxylase at low ionic strength followed by Sephadex gel filtration resolves apoenzyme from cofactor (>90%)...
Published in Neurochemical research
Published in Neurochemical research
3-Methoxy-4-hydroxymandelic acid (VMA) is transported into the isolated choroid plexus against a concentration gradient by a saturable, energy-dependent system. The apparentK m for transport is 35 nM and theV max is 1 pmol/mg tissue/hr. Concentrations of probenecid (0.1 mM) that block the transport of other acidic biogenic amine metabolites did not...
Published in Neurochemical research
A flotation technique for the rapid preparation of synaptosomes, originally developed for invertebrate nervous tissue, has now been successfully applied to that of an elasmobranch fish (Mustelis canis, dogfish). The technique involves submitting the supernatant, obtained after a homogenate has been centrifuged at low speed to remove nuclei and tiss...
Published in Neurochemical research
Thiamine-deficient rats were injected with [(35)S]thiamine and the turnover of the injected radioactive thiamine and its phosphate esters was measured in brain, heart, and liver after returning the rats to a normal diet. The radioactivity per gram wet tissue, as well as the specific activity, fell to half its initial value in less than 40 hr, showi...
Published in Neurochemical research
