Rubalcava-Gracia, Diana García-Villegas, Rodolfo Larsson, Nils-Göran
Published in
Molecular cell
Although the mammalian mtDNA transcription machinery is simple and resembles bacteriophage systems, there are many reports that nuclear transcription regulators, as exemplified by MEF2D, MOF, PGC-1α, and hormone receptors, are imported into mammalian mitochondria and directly interact with the mtDNA transcription machinery. However, the supporting ...
Rodríguez-Molina, Juan B. West, Steven Passmore, Lori A.
Published in
Molecular cell
Gene expression is controlled in a dynamic and regulated manner to allow for the consistent and steady expression of some proteins as well as the rapidly changing production of other proteins. Transcription initiation has been a major focus of study because it is highly regulated. However, termination of transcription also plays an important role i...
Warren, Gus D Kitao, Tomoe Franklin, Tyler G Nguyen, Justine V Geurink, Paul P Kubori, Tomoko Nagai, Hiroki Pruneda, Jonathan N
Published in
Molecular cell
The versatility of ubiquitination to control vast domains of eukaryotic biology is due, in part, to diversification through differently linked poly-ubiquitin chains. Deciphering signaling roles for some chain types, including those linked via K6, has been stymied by a lack of specificity among the implicated regulatory proteins. Forged through stro...
Daniel, Bence Belk, Julia A Meier, Stefanie L Chen, Andy Y Sandor, Katalin Czimmerer, Zsolt Varga, Zsofia Bene, Krisztian Buquicchio, Frank A Qi, Yanyan
...
Published in
Molecular cell
Cell cycle (CC) facilitates cell division via robust, cyclical gene expression. Protective immunity requires the expansion of pathogen-responsive cell types, but whether CC confers unique gene expression programs that direct the subsequent immunological response remains unclear. Here, we demonstrate that single macrophages (MFs) adopt different pla...
Kochavi, Adva Lovecchio, Domenica Faller, William James Agami, Reuven
Published in
Molecular cell
mRNA translation is a highly conserved and tightly controlled mechanism for protein synthesis and is well known to be altered by oncogenes to promote cancer development. This distorted mRNA translation is accompanied by the vulnerability of cancer to inhibitors of key mRNA translation components. Novel studies also suggest that these alternations c...
Thoresen, Daniel T Galls, Drew Götte, Benjamin Wang, Wenshuai Pyle, Anna M
Published in
Molecular cell
RIG-I is essential for host defense against viral pathogens, as it triggers the release of type I interferons upon encounter with viral RNA molecules. In this study, we show that RIG-I is rapidly and efficiently activated by small quantities of incoming viral RNA and that it relies exclusively on the constitutively expressed resident pool of RIG-I ...
Song, Jinghui Dong, Liting Sun, Hanxiao Luo, Nan Huang, Qiang Li, Kai Shen, Xiaowen Jiang, Zhe Lv, Zhicong Peng, Luxin
...
Published in
Molecular cell
Nonsense mutations, accounting for >20% of disease-associated mutations, lead to premature translation termination. Replacing uridine with pseudouridine in stop codons suppresses translation termination, which could be harnessed to mediate readthrough of premature termination codons (PTCs). Here, we present RESTART, a programmable RNA base editor, ...
Ugolini, Ilaria Bilokapic, Silvija Ferrolino, Mylene Teague, Josiah Yan, Yinxia Zhou, Xuelin Deshmukh, Ashish White, Michael Kriwacki, Richard W Halic, Mario
...
Published in
Molecular cell
Ribosome biogenesis takes place in the nucleolus, a nuclear membrane-less organelle. Although well studied, it remains unknown how nascent ribosomal subunits separate from the central chromatin compartment and move to the outer granular component, where maturation occurs. We find that the Schizosaccharomyces pombe nucleophosmin-like protein Fkbp39 ...
Zeng, Yi Fair, Benjamin J Zeng, Huilin Krishnamohan, Aiswarya Hou, Yichen Hall, Johnathon M Ruthenburg, Alexander J Li, Yang I Staley, Jonathan P
Published in
Molecular cell
Long introns with short exons in vertebrate genes are thought to require spliceosome assembly across exons (exon definition), rather than introns, thereby requiring transcription of an exon to splice an upstream intron. Here, we developed CoLa-seq (co-transcriptional lariat sequencing) to investigate the timing and determinants of co-transcriptiona...
van Vliet, Alexander R Chiduza, George N Maslen, Sarah L Pye, Valerie E Joshi, Dhira De Tito, Stefano Jefferies, Harold B J Christodoulou, Evangelos Roustan, Chloë Punch, Emma
...
Published in
Molecular cell
ATG9A and ATG2A are essential core members of the autophagy machinery. ATG9A is a lipid scramblase that allows equilibration of lipids across a membrane bilayer, whereas ATG2A facilitates lipid flow between tethered membranes. Although both have been functionally linked during the formation of autophagosomes, the molecular details and consequences ...