Holland, Alexandra D. Rückerl, Florian Dragavon, Joseph M. Rekiki, Abdessalem Tinevez, Jean-Yves Tournebize, Régis Shorte, Spencer L.
Published in
Methods
Energy transfer mechanisms represent the basis for an array of valuable tools to infer interactions in vitro and in vivo, enhance detection or resolve interspecies distances such as with resonance. Based upon our own previously published studies and new results shown here we present a novel framework describing for the first time a model giving a v...
Ramanujan, V. Krishnan
Published in
Methods
We report here a novel combination of time-resolved imaging methods for probing mitochondrial metabolism in multiple time scales at the level of single cells. By exploiting a mitochondrial membrane potential reporter fluorescence we demonstrate the single cell metabolic dynamics in time scales ranging from microseconds to seconds to minutes in resp...
Zarrabi, Nawid Ernst, Stefan Verhalen, Brandy Wilkens, Stephan Börsch, Michael
Published in
Methods
Single-molecule Förster resonance energy (smFRET) transfer has become a powerful tool for observing conformational dynamics of biological macromolecules. Analyzing smFRET time trajectories allows to identify the state transitions occuring on reaction pathways of molecular machines. Previously, we have developed a smFRET approach to monitor movement...
Gahl, Robert F. Tekle, Ephrem Tjandra, Nico
Published in
Methods
Translocation of proteins to different parts of the cell is necessary for many cellular mechanisms as a means for regulation and a variety of other functions. Identifying how these proteins undergo conformational changes or interact with various partners during these events is critical to understanding how these mechanisms are executed. A protocol ...
Bockaert, Joël Claeysen, Sylvie Bécamel, Carine Dumuis, Aline Marin, Philippe
Published in
Cell and Tissue Research
Serotonin (5-hydroxytryptamine, 5-HT) is, without doubt, the neurotransmitter for which the number of receptors is the highest. Fifteen genes encoding functional 5-HT receptors have been cloned in mammalian brain. 5-HT3 receptors are ionotropic receptors, whereas all the others are metabotropic G-protein-coupled receptors (GPCRs). 5-HT receptor div...
Day, Richard N.
Published in
Methods
The method of fluorescence lifetime imaging microscopy (FLIM) is a quantitative approach that can be used to detect Förster resonance energy transfer (FRET). The use of FLIM to measure the FRET that results from the interactions between proteins labeled with fluorescent proteins (FPs) inside living cells provides a non-invasive method for mapping i...
Periasamy, Ammasi
Published in
Methods
Mergenthaler, Philipp Wendland, Kristin Meisel, Andreas
Published in
Methods
To understand the principles that govern mechanisms of neuronal survival or death it is necessary to systematically model these processes. Methods involving overexpression or knockdown of a gene of interest using non-viral transfection of primary neurons can easily be adapted to study cell death pathways in primary neurons. However, common biochemi...
Canclini, Lucía Wallrabe, Horst Di Paolo, Andrés Kun, Alejandra Calliari, Aldo Sotelo-Silveira, José Roberto Sotelo, José Roberto
Published in
Methods
Evidence from multiple sources supports the hypothesis that Schwann cells in the peripheral nervous system transfer messenger RNA and ribosomes to the axons they ensheath. Several technical and methodological difficulties exist for investigators to unravel this process in myelinated axons – a complex two-cell unit. We present an experimental design...
Niederberger, Verena Eckl-Dorna, Julia Pauli, Gabrielle
Published in
Methods
Over the last 25years, recombinant allergens from all important allergen sources have been cloned and are now available as recombinant proteins. These molecules can be produced in practically unlimited amounts without biological or batch-to-batch variability. It has been shown in provocation tests that recombinant allergens have similar clinical ef...
