Gonzalez-Nino, David Sonntag, Steven Afshar-Mohajer, Mahyar Goss, Josh Zou, Min Prinz, Gary S
Published in
Journal of visualized experiments : JoVE
This study presents a methodology for the rapid fabrication and micro-tensile testing of additively manufactured (AM) 17-4PH stainless steels by combining photolithography, wet-etching, focused ion beam (FIB) milling, and modified nanoindentation. Detailed procedures for proper sample surface preparation, photo-resist placement, etchant preparation...
Russ, Martin Boerger, Emilia von Platen, Philip Francis, Roland C E Taher, Mahdi Boemke, Willehad Lachmann, Burkhard Leonhardt, Steffen Pickerodt, Philipp A
Published in
Journal of visualized experiments : JoVE
Various animal models exist to study the complex pathomechanisms of the acute respiratory distress syndrome (ARDS). These models include pulmo-arterial infusion of oleic acid, infusion of endotoxins or bacteria, cecal ligation and puncture, various pneumonia models, lung ischemia/reperfusion models and, of course, surfactant depletion models, among...
Shechtman, Lauren A Piarowski, Christina M Scott, Jennifer K Golden, Erin J Gaillard, Dany Barlow, Linda A
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Journal of visualized experiments : JoVE
The sense of taste is mediated by taste buds on the tongue, which are composed of rapidly renewing taste receptor cells (TRCs). This continual turnover is powered by local progenitor cells and renders taste function prone to disruption by a multitude of medical treatments, which in turn severely impacts the quality of life. Thus, studying this proc...
Ng, Inn Chuan Zhang, Li Shen, Narelle Nichola Yi Ying Soong, Yun Ting Ng, Chan Way Koh, Phoebe Kang Sheing Zhou, Yan Yu, Hanry
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Journal of visualized experiments : JoVE
Primary hepatocytes are widely used in basic research on liver diseases and for toxicity testing in vitro. The two-step collagenase perfusion procedure for primary hepatocyte isolation is technically challenging, especially in portal vein cannulation. The procedure is also prone to occasional contamination and variations in perfusion conditions due...
Petersen, Rebecca A Morris, Ann C
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Journal of visualized experiments : JoVE
Vertebrate eye development is a complex process that begins near the end of embryo gastrulation and requires the precise coordination of cell migration, proliferation, and differentiation. Time-lapse imagining offers unique insight to the behavior of cells during eye development because it allows us to visualize oculogenesis in vivo. Zebrafish are ...
Lavorato, Manuela Mathew, Neal D Shah, Nina Nakamaru-Ogiso, Eiko Falk, Marni J
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Journal of visualized experiments : JoVE
Caenorhabditis elegans is widely recognized for its central utility as a translational animal model to efficiently interrogate mechanisms and therapies of diverse human diseases. Worms are particularly well-suited for high-throughput genetic and drug screens to gain deeper insight into therapeutic targets and therapies by exploiting their fast deve...
Oderberg, Isaac M Goessling, Wolfram
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Journal of visualized experiments : JoVE
Liver failure is one of the leading causes of death worldwide, and mortality from chronic liver disease is rising sharply in the United States. Healthy livers are capable of regenerating from toxic damage, but in advanced liver disease, the natural ability of the liver to regenerate is impaired. Zebrafish have emerged as a powerful experimental sys...
Chen See, Jeremy R Wright, Olivia Unverdorben, Lavinia V Heibeck, Nathan Techtmann, Stephen M Hazen, Terry C Lamendella, Regina
Published in
Journal of visualized experiments : JoVE
Hydraulic fracturing (HF), commonly called "fracking", uses a mixture of high-pressure water, sand, and chemicals to fracture rocks, releasing oil and gas. This process revolutionized the U.S. energy industry, as it gives access to resources that were previously unobtainable and now produces two-thirds of the total natural gas in the United States....
Comfort, Nicole Cai, Kunheng Bloomquist, Tessa R. Strait, Madeleine D. Ferrante, Anthony W. Jr. Baccarelli, Andrea A.
Published in
Journal of visualized experiments : JoVE
The physiological and pathophysiological roles of extracellular vesicles (EVs) have become increasingly recognized, making the EV field a quickly evolving area of research. There are many different methods for EV isolation, each with distinct advantages and disadvantages that affect the downstream yield and purity of EVs. Thus, characterizing the E...
Her, Zuag Paj Yeo, Kok Siong Howe, Cassie Levee, Taylor Zhu, Shizhen
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Journal of visualized experiments : JoVE
Zebrafish has emerged as an important animal model to study human diseases, especially cancer. Along with the robust transgenic and genome editing technologies applied in zebrafish modeling, the ease of maintenance, high-yield productivity, and powerful live imaging altogether make the zebrafish a valuable model system to study metastasis and cellu...
