Boyd, April E Allegood, Jeremy Lima, Santiago
Published in
Journal of visualized experiments : JoVE
Sphingolipids are cellular components that have well-established roles in human metabolism and disease. Mass spectrometry can be used to determine whether sphingolipids are altered in a disease and investigate whether sphingolipids can be targeted clinically. However, properly powered prospective studies that acquire tissues directly from the surgi...
Fong, Daryl H Cohen, Adrian J Mahony, Dylan E Simon, Neil G Herrera, Joseph E Baron, Rebecca B Putrino, David
Published in
Journal of visualized experiments : JoVE
A portable system capable of measuring steady-state visual-evoked potentials (SSVEP) was developed to provide an objective, quantifiable method of electroencephalogram (EEG) testing following a traumatic event. In this study, the portable system was used on 65 healthy rugby players throughout a season to determine whether SSVEP are a reliable elect...
Sartori, Federica M. O. Buzby, Cassandra Plavskin, Yevgeniy Siegal, Mark L.
Published in
Journal of visualized experiments : JoVE
Yeast growth phenotypes are precisely measured through highly parallel time-lapse imaging of immobilized cells growing into microcolonies. Simultaneously, stress tolerance, protein expression, and protein localization can be monitored, generating integrated datasets to study how environmental and genetic differences, as well as gene-expression hete...
Zhao, Rongwei Chenoweth, David M. Zhang, Huaiying
Published in
Journal of visualized experiments : JoVE
This protocol illustrates a chemically induced protein dimerization system to create condensates on chromatin. The formation of promyelocytic leukemia (PML) nuclear body on telomeres with chemical dimerizers is demonstrated. Droplet growth, dissolution, localization and composition are monitored with live cell imaging, immunofluorescence (IF) and f...
Sheard, Kelsey M. Cox, Rachel T.
Published in
Journal of visualized experiments : JoVE
The objective of this protocol is to use live imaging to visualize the effects of oxidative damage on the localization and dynamics of subcellular structures in Drosophila ovaries.
Liao, Lucia M Q Gray, Rachel A V Martin, Dale D O
Published in
Journal of visualized experiments : JoVE
Fatty acylation, the covalent addition of saturated fatty acids to protein substrates, is important in regulating a myriad of cellular functions in addition to its implications in cancer and neurodegenerative diseases. Recent developments in fatty acylation detection methods have enabled efficient and non-hazardous detection of fatty acylated prote...
Gray, Michael Vasilyeva, Alexandra V Brans, Veerle Stride, Eleanor
Published in
Journal of visualized experiments : JoVE
Interest in the therapeutic applications of ultrasound is significant and growing, with potential clinical targets ranging from cancer to Alzheimer's disease. Cavitation - the formation and subsequent motion of bubbles within an ultrasound field - represents a key phenomenon underpinning many of these treatments. There remains, however, considerabl...
Borrello, Alessandro Agnes, Amedea Luciana Pellegrino, Eleonora Magalini, Sabina Gui, Daniele
Published in
Journal of visualized experiments : JoVE
The superior mesenteric artery can be cannulated in humans through minimally invasive radiological catheterization of the femoral or axillary artery. SMA cannulation is more difficult in rats due to small anatomical dimensions. The aim of the study is to describe a surgical technique for cannulation of the SMA in rats to perform long-term infusion ...
de Martín Garrido, Natàlia Ramlaul, Kailash Aylett, Christopher H S
Published in
Journal of visualized experiments : JoVE
Structure determination by cryo-electron microscopy (cryo-EM) has rapidly grown in the last decade; however, sample preparation remains a significant bottleneck. Macromolecular samples are ideally imaged directly from random orientations in a thin layer of vitreous ice. However, many samples are refractory to this, and protein denaturation at the a...
Parker, M Rockwell Currylow, Andrea F Tillman, Eric A Robinson, Charlotte J Josimovich, Jillian M Bukovich, Isabella M G Nazarian, Lauren A Nafus, Melia G Kluever, Bryan M Adams, Amy Y Yackel
...
Published in
Journal of visualized experiments : JoVE
Reptiles utilize a variety of environmental cues to inform and drive animal behavior such as chemical scent trails produced by food or conspecifics. Decrypting the scent-trailing behavior of vertebrates, particularly invasive species, enables the discovery of cues that induce exploratory behavior and can aid in the development of valuable basic and...
