Microsporidiosis in a lovebird.
Brucella suis biovar 3 infection in a Kentucky swine herd.
Sows from a large farrow-to-finish operation in western Kentucky had late-term abortions. Boars and breeding-age sows were tested serologically for brucellosis, and 83 of 125 were classified as reactors. No brucellae were isolated from the tissues of 6 unbred reactor sows, but Brucella suis biovar 3 was recovered from 5 aborted fetuses. Epidemiolog...
Actinobacillus (Haemophilus) pleuropneumoniae: use of coagglutination and complement fixation to determine the relations...
The conventional culture method was compared to coagglutination for detection of Actinobacillus (Haemophilus) pleuropneumoniae in 425 sets of pig lungs. Sera from the same animals were evaluated for antibodies to A. pleuropneumoniae by the complement fixation (CF) test. All samples were collected at 2 packing plants in Iowa. In 2 nonvaccinated herd...
Subcutaneous pythiosis in a dog.
Detection of bovine viral diarrhea virus by spot hybridization with probes prepared from cloned cDNA sequences.
A cytopathic strain of bovine viral diarrhea virus (BVDV) was purified from infected cell culture fluids by isopycnic density-gradient centrifugation. Genomic RNA was extracted and tailed with adenine residues at the 3' end with poly-A polymerase. Double-stranded complementary DNA (cDNA) was synthesized, using the poly-A-tailed RNA as a template an...
Laboratory investigation of a naturally occurring outbreak of arthrogryposis-hydranencephaly in Texas sheep.
An epizootic of abortions, weak lambs, stillbirths, and congenital arthrogryposis-hydranencephaly occurred in a sheep flock in West Texas. The outbreak began during the first week of January 1987 and continued through the third week of February 1987. Lambs born after February 1987 were not affected. A high incidence of antibodies to Cache Valley vi...
Elevated IgG antibody to Sarcocystis cruzi associated with eosinophilic myositis in cattle.
Blood sera, skeletal muscle, and cardiac muscle from 24 bovine carcasses condemned for eosinophilic myositis by US Department of Agriculture meat inspection veterinarians were compared with similar specimens from 35 random carcasses passed for human consumption. Fluorescence values determined by using a fluorometric immunoassay system were used to ...
Diagnostic investigation of bovine viral diarrhea infection in a Minnesota dairy herd.
Bovine viral diarrhea (BVD) virus infection was diagnosed in neonatal calves with enteritis. Successful diagnostic procedures included direct immunofluorescence of frozen tissue sections, histopathology, and virus isolation. Virus isolation from buffy coats and serum was successful in detecting infected animals, whereas direct immunofluorescence of...
E.P. Pope Memorial Award to Dr. Glenn H. Snoeyenbos.
Rapid fluorescence detection of in situ hybridization with biotinylated bovine herpesvirus-1 DNA probes.
The use of biotinylated DNA hybridization probes for clinical detection of bovine herpesvirus-1 was investigated. Biotinylated DNA hybridization probes were prepared from bovine herpesvirus-1 DNA purified from infected cell cultures. The viral DNA was nick translated in the presence of biotin-dUTP with DNA polymerase to incorporate biotin into the ...
