Gniadek, Thomas J Carroll, Karen C Simner, Patricia J
Published in
Journal of clinical microbiology
The non-glucose-fermenting Gram-negative bacilli Pseudomonas aeruginosa and Acinetobacter baumannii are increasingly acquiring carbapenem resistance. Given their intrinsic antibiotic resistance, this can cause extremely difficult-to-treat infections. Additionally, resistance gene transfer can occur between Gram-negative species, regardless of their...
Herbst de Cortina, S Bristow, C C Vargas, S K Perez, D G Konda, K A Caceres, C F Klausner, J D
Published in
Journal of clinical microbiology
Combining the detection of syphilis and HIV antibodies into one point-of-care test integrates syphilis screening into already existing HIV screening programs, which may be particularly beneficial in settings such as antenatal care. Using the INSTI Multiplex downward-flow immunoassay, we tested 200 stored serum samples from high-risk patients enroll...
Tato, M Ruiz-Garbajosa, P Traczewski, M Dodgson, A McEwan, A Humphries, R Hindler, J Veltman, J Wang, H Cantón, R
...
Published in
Journal of clinical microbiology
Rapid identification of patients who are colonized with carbapenemase-producing organisms (CPO) is included in multiple national guidelines for containment of these organisms. In a multisite study, we evaluated the performance of the Cepheid Xpert Carba-R assay, a qualitative diagnostic test that was designed for the rapid detection and differentia...
Borman, Andrew M Desnos-Ollivier, Marie Campbell, Colin K Bridge, Paul D Dannaoui, Eric Johnson, Elizabeth M
Published in
Journal of clinical microbiology
Eumycetoma is a debilitating, chronic, fungal infection that is endemic in India, Indonesia, and parts of Africa and South and Central America. It remains a neglected tropical disease in need of international recognition. Infections follow traumatic implantation of saprophytic fungi and frequently require radical surgery or amputation in the absenc...
Toliman, P Badman, S G Gabuzzi, J Silim, S Forereme, L Kumbia, A Kombuk, B Kombati, Z Allan, J Munnull, G
...
Published in
Journal of clinical microbiology
The World Health Organization has recommended that testing for high-risk human papillomavirus (HPV) (hrHPV) infection be incorporated into cervical screening programs in all settings worldwide. In many high-burden, low-income countries, it will not be feasible to achieve high cervical screening coverage using hrHPV assays that require clinician-col...
Thomson, Richard B Jr
Published in
Journal of clinical microbiology
The Gram stain is one of the most commonly performed tests in the clinical microbiology laboratory, yet it is poorly controlled and lacks standardization. It was once the best rapid test in microbiology, but it is no longer trusted by many clinicians. The publication by Samuel et al. (J. Clin. Microbiol. 54:1442-1447, 2016, http://dx.doi.org/10.112...
Samuel, Linoj P Balada-Llasat, Joan-Miquel Harrington, Amanda Cavagnolo, Robert
Published in
Journal of clinical microbiology
Gram stains remain the cornerstone of diagnostic testing in the microbiology laboratory for the guidance of empirical treatment prior to availability of culture results. Incorrectly interpreted Gram stains may adversely impact patient care, and yet there are no comprehensive studies that have evaluated the reliability of the technique and there are...
Kristiansen, Gitte Qvist Lisby, Jan Gorm Schønning, Kristian
Published in
Journal of clinical microbiology
Rapid and sensitive detection of macrolide resistance in Mycoplasma genitalium is required for the guidance of adequate antimicrobial treatment. Previous studies have confirmed that single-base mutations at position 2058 or 2059 in domain V of the 23S rRNA gene of M. genitalium result in high-level macrolide resistance. Sequencing of PCR products r...
Park, Kyung-Hwa Greenwood-Quaintance, Kerryl E Hanssen, Arlen D Abdel, Matthew P Patel, Robin
Published in
Journal of clinical microbiology
We compared culture results to investigate the influence of antimicrobial-loaded cement on sonicate fluid culture positivity for the diagnosis of prosthetic joint infection. Fifty-four subjects were assessed. The sensitivities of sonicate fluid culture were 77.8% (14 of 18) in subjects with an antimicrobial-loaded cemented prosthesis and 58.3% (21 ...
Chikeka, Ijeuru Matute, Armando J Dumler, J Stephen Woods, Christopher W Mayorga, Orlando Reller, Megan E
Published in
Journal of clinical microbiology
Ehrlichia chaffeensis, the etiologic agent of human monocytic ehrlichiosis (HME), has been extensively studied as a cause of acute febrile illness and an emerging tick-borne zoonosis in the United States. Limited data suggest its presence in other regions, including Central and South America but not Nicaragua to date. Diagnosis of E. chaffeensis in...
