Published in Journal of clinical immunology
Aliquots of human blood mononuclear cells were cultured in vitro in the absence or presence of varying concentrations of histamine (10(-3)-10(-8) M) for 24 hr, mitomycin treated, washed, and cocultured with autologous indicator cells that were then stimulated by PHA. The degree of suppression of thymidine uptake was measured in the presence of hist...
Published in Journal of clinical immunology
The mechanisms responsible for the increased serum immunoglobulines in polyclonal gammopathy states are unknown. We have studied the regulation of peripheral blood B-cell immunoglobulin synthesis and secretion, by T cells of either blood or bone marrow compartments, in 20 patients with polyclonal gammopathy and 27 controls with normal serum immunog...
Published in Journal of clinical immunology
An absolute requirement for monocytes was demonstrated in the T-cell proliferative response to tetanus toxoid (TT) antigen. Antigen-pulsed monocytes were shown to be effective in triggering T-cell proliferation. Using 125I-radiolabeled TT antigen, uptake by monocytes increased progressively over an 18-hr period, at which time 80-85% of the monocyte...
Published in Journal of clinical immunology
Mononuclear cells from 39 patients with hypogammaglobulinemia of the common variable type were analyzed for in vitro proliferative response to a panel of cell activators in order to examine the lymphocyte response to mitogens and to study the capacity to generate an immunologically specific secondary response. Patient lymphocyte response to phytohe...
Published in Journal of clinical immunology
We have compared the effects of the synthetic chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine (f-Met-Leu-Phe), on the deformability and chemotaxis of human polymorphonuclear leukocytes (PMNs). By utilizing the technique of cell elastimetry (deformability), which measures the negative pressure required to aspirate cells into a micropipe...
Published in Journal of clinical immunology
Published in Journal of clinical immunology
Sequential studies of cellular and humoral immunity were conducted in an infant with the Wiskott-Aldrich syndrome prior to and after a splenectomy for uncontrollable hemorrhage. All measures of cellular immunity showed gradual improvement during the 8-month period after surgery. Serum isohemagglutinins, diphtheria and tetanus antibodies, and the pe...
Published in Journal of clinical immunology
On analysis of in vitro assays of human natural killer (NK) cell function the inadequacy of commonly used methods of expressing lytic activity was apparent. A comparison was made of the data obtained using modifications of two equations-the simple exponential fit and the von Krogh equations. Both of these equations were found to satisfy the followi...
Published in Journal of clinical immunology
The results of a nationwide survey on primary immunodeficiency syndrome (PIS) in Japan are presented. By the repeated questionnaire method, 497 PIS cases were collected prior to February 1979 with clinical information. Numbers of each type of PIS, age at the time of diagnosis, patient's status at the time of registration, familial incidence of PIS,...
Published in Journal of clinical immunology
The objective of this study was to identify and test a convenient means for long-term storage of lymphocytes taken from clinically characterized patients without losing B- or T-cell function. Accordingly, peripheral blood lymphocytes were frozen and stored, and portions of each sample were subsequently assayed for T-cell blastogenic response and B-...
