Baba, Y Tsuhako, M Sawa, T Akashi, M
Published in
Journal of chromatography. A
Base-specific separations of oligodeoxynucleotides were achieved with high resolution by electrophoresis, using a urea-gel capillary, in which poly(9-vinyladenine) (PVAd) was utilized as an affinity ligand. The migration behaviour and the plate number of oligodeoxynucleotides were investigated as a function of urea concentration between 2 and 10 M ...
Tadey, T Purdy, W C
Published in
Journal of chromatography. A
The influence of various detergents on the capillary electrophoretic behaviour of plasma apolipoproteins was studied. Electrophoretic mobility increased in the presence of anionic detergents sodium deoxycholate (DOC) and sodium dodecyl sulfate (SDS), and decreased in the presence of non-ionic Triton X-100. Apolipoproteins from plasma low-density li...
Izumi, T Yamaguchi, M Yoneda, K Isobe, T Okuyama, T Shinoda, T
Published in
Journal of chromatography. A
Glucomannan, a natural polysaccharide extracted from Amorphophallus konjac, was employed as a sieving additive for the separation of restriction DNA fragments in capillary electrophoresis. A stable solution of entangled glucomannan was successfully prepared under mild conditions. The separation capillary filled with 0.25% glucomannan exhibited exce...
Schmalzing, D Piggee, C A Foret, F Carrilho, E Karger, B L
Published in
Journal of chromatography. A
Polyvinylmethylsiloxanediol (50% vinyl) was synthesized and combined with a cross-linker for static coating onto fused-silica columns. After cross-linking and binding to the surface, linear polyacrylamide was grafted to the double bonds of the siloxanediol; subsequently, this linear polymer matrix was cross-linked with formaldehyde. The grafted neu...
Arentoft, A M Frøkiaer, H Michaelsen, S Sørensen, H Sørensen, S
Published in
Journal of chromatography. A
High-performance capillary electrophoresis (HPCE) was adapted for the determination of Kunitz soybean trypsin inhibitor, Bowman Birk inhibitor from soybean and protein-type proteinase inhibitors from pea (Pisum sativum L.). The method was developed for the determination and characterization of the inhibitors, the enzymes trypsin and chymotrypsin an...
de Jong, N Visser, S Olieman, C
Published in
Journal of chromatography. A
The determination of milk proteins by capillary zone electrophoresis (CZE) is hampered by the adsorption of the solute on the capillary wall. The effects of pH, ionic strength of the buffer and polymeric additives were studied in combination with a hydrophilically coated capillary. Optimum separations were obtained at low pH (2.5-3) in aqueous solu...
Yowell, G G Fazio, S D Vivilecchia, R V
Published in
Journal of chromatography. A
Esaka, Y Yamaguchi, Y Kano, K Goto, M
Published in
Journal of chromatography. A
Condensation products (oxazole derivatives) from the reaction of the coenzyme pyrroloquinoline quinone (PQQ) with several alpha-amino acids were successfully separated by capillary zone electrophoresis. Addition of a certain organic solvent such as dimethyl sulphoxide to the electrolyte solution is essential for reproducible and complete separation...
Barron, A E Soane, D S Blanch, H W
Published in
Journal of chromatography. A
We have used dilute and semi-dilute uncross-linked hydroxyethyl cellulose (HEC) solutions as separation matrices for capillary electrophoresis of DNA restriction fragments. In these experiments, we investigated the effects of HEC molecular weight and concentration on resolution, attempting to relate these parameters to the polymer entanglement thre...
Pariat, Y F Berka, J Heiger, D N Schmitt, T Vilenchik, M Cohen, A S Foret, F Karger, B L
Published in
Journal of chromatography. A
The use of low percent (1.5-6% T) replaceable linear polyacrylamide (LPA) network matrices for rapid separation of double-stranded DNA fragments was explored. Separations of fragments ranging from 20 to 23,000 base pairs were readily achieved. Typically, 4 x 10(6) theoretical plates/m were obtained in less than 30 min. Short separation times under ...
