Zhang, Hairong Jiang, Xiaojuan Zhang, Dandan Yang, Yuexin Xie, Qiang Wu, Caisheng
Published in
Journal of chromatography. A
Analysis of exposure to traditional Chinese medicine (TCM) in vivo based on mass spectrometry is helpful for the screening of effective ingredients of TCM and the development of new drugs. The method of screening biomarkers through metabolomics technology is a nontargeted research method to explore the differential components between two sets of bi...
Bozza, Desiree De Luca, Chiara Felletti, Simona Spedicato, Matteo Presini, Francesco Giovannini, Pier Paolo Carraro, Marco Macis, Marco Cavazzini, Alberto Catani, Martina
...
Published in
Journal of chromatography. A
Preparative liquid chromatography in reversed phase conditions (RPLC) is the most common approach adopted in the downstream processing for the purification of therapeutic peptides at industrial level. Due to the strict requirements on the quality imposed by the Regulatory Agencies, routinary methods based on the use of aqueous buffers and acetonitr...
Redón, Lídia Subirats, Xavier Chapel, Soraya Januarius, Timothy Broeckhoven, Ken Rosés, Martí Cabooter, Deirdre Desmet, Gert
Published in
Journal of chromatography. A
A detailed analysis of intra-particle volumes and layer thicknesses and their effect on the diffusion of solutes in hydrophilic interaction liquid chromatography (HILIC) was made. Pycnometric measurements and the retention volume of deuterated mobile phase constituents (water and acetonitrile) were used to estimate the void volume inside the column...
Lin, Jiana Xie, Zenghui Hu, Yuling Li, Gongke Zhong, Qisheng
Published in
Journal of chromatography. A
Supramolecular macrocycle-based covalent organic frameworks (COFs) are promising adsorbents for adsorption of hazards due to their host-guest recognition property. However, most supramolecular macrocycles are conformationally flexible, making them challenging to introduce into COFs. In this work, a calix[6]arene-based COF (CX6-BD COF) was fabricate...
Huang, Zongqing Hua, Haoju Du, Xiuzhen Zhen, Zipeng Zhao, Wei Feng, Jun Li, Ji-An
Published in
Journal of chromatography. A
As an excellent fusion tag for expressing heterologous proteins, yeast SUMO (small ubiquitin-related modifier) has unique advantages such as improving solubility, promoting stability, and reducing degradation, but it lacks a simple and rapid purification method. Camelid single-domain antibodies (VHHs or nanobodies) show great promise as an efficien...
Qiu, Xueting Hou, Xuben Yang, Yue Fang, Hao Cui, Fei Yang, Xinying
Published in
Journal of chromatography. A
Protein tyrosine phosphatase receptor type O (PTPRO) plays an important role in inflammation-related pathways and has become an emerging drug target. In this study, we developed an in-line capillary electrophoresis (CE) method for the investigation of the enzymatic activity of PTPRO, which was based on electrophoretically mediated microanalysis (EM...
Vosáhlová, Zuzana Kalíková, Květa Gilar, Martin Szymarek, Jakub Mazurkiewicz-Bełdzińska, Maria Studzińska, Sylwia
Published in
Journal of chromatography. A
With the development of therapeutic oligonucleotides for antisense and gene therapies, the demand for analytical methods also increases. For the analysis of complex samples, for example plasma samples, where the use of mass detection is essential, hydrophilic interaction liquid chromatography is a suitable choice. The aim of the present work was to...
Sun, Yangkun Kuang, Jingjing Cheng, Yongzhe Lin, Chuhui Zhang, Hongyang Zhang, Min Ning, Fanghong Hu, Ping
Published in
Journal of chromatography. A
Long-term intake of animal-derived foods with excessive fluoroquinolones (FQs) will cause damage to human health, so it is critical to establish a feasible approach for sensitive and rapid monitoring of FQs residues. In this study, a new cyclodextrin modified magnetic metal-organic frameworks (Fe3O4@UiO-66-CD) was successfully synthesized by amidat...
Kongasseri, Aswanidevi Madhesan, Thirumalai Krishna Kumar, Sangeetha Pedugu Sivaraman, Sushmitha Mitra, Suchashrita Kancharlapalli Chinaraga, Pitchaiah Rao, C V S Brahmmananda Nagarajan, Sivaraman Deivasigamani, Prabhakaran Mohan, Akhila Maheswari
...
Published in
Journal of chromatography. A
The current work focuses on the sequential separation of trivalent lanthanides (except Pm3+) using modified C18 silica-packed supports through the reversed-phase high-performance liquid chromatography (RP-HPLC) technique. In the current research, four indigenously synthesized amphiphilic aromatic triamide derivatives, namely N1, N1, N3, N3, N5, N5-...
Wen, Zeng Peng, Ruihan Gao, Die Lin, Jing Zeng, Jia Li, Zhou Ke, Famin Xia, Zhining Wang, Dandan
Published in
Journal of chromatography. A
A chitosan-alginate sponge (CAS) with multiple cross-linking networks was developed using chitosan, sodium alginate, polyvinyl alcohol, and glutaraldehyde to adsorb and enrich the anionic dyes form the food samples. The multiple networks in CAS refer to the electrostatic cross-linking network, hydrogen bonding cross-linking network, and covalent cr...
