Publication search

Adaptation of human enteric coronavirus to growth in cell lines.

Published in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

The existence of human enteric coronavirus (HEC) has been debated since its first description in stool by electron microscopy (EM) in 1975. Needed to resolve the issue is its cultivation in readily available cell lines. To grow HEC in cell lines. To describe its characteristics and to differentiate it from other human and animal coronaviruses. Orig...

Diagnostic electron microscopy is still a timely and rewarding method.

Published in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

Parallel to its technical development starting in the 1930s, electron microscopy (EM) became an important tool in basic and clinical virology. First utilized in the rapid diagnosis of smallpox, it developed to a diagnostic routine in the early 1960s using the negative staining technique. EM was applied to infected cell-cultures and also to 'dirty' ...

Detection of viral, Chlamydia pneumoniae and Mycoplasma pneumoniae infections in exacerbations of asthma in children.

Published in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

A high frequency of virus infections has been recently pointed out in the exacerbations of asthma in children. To confirm this, using conventional and molecular detection methods, and expanding the study to younger children. One hundred and thirty-two nasal aspirates from 75 children hospitalized for a severe attack of asthma were studied (32 infan...

Lowered yields of virus-induced interferon production in leukocyte cultures and risk of recurrent respiratory infections...

Published in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

To study the correlation between the yield of virus-induced interferon (IFN) production in leukocyte cultures and the risk of recurrent respiratory infections. A sample of 71 consecutive children enrolled in the Finnish Otitis Media Cohort Study were selected. Children suffering from frequently recurring respiratory infections (FRRIs) were defined ...

A history of the Pan American Society for Clinical Virology.

Published in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

Rapid multiserotype detection of human rhinoviruses on optically coated silicon surfaces.

Published in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

More than 100 immunologically distinct serotypes of human rhinoviruses (HRV) have been discovered, making detection of surface exposed capsid antigens impractical. However, the non-structural protein 3C protease (3Cpro) is essential for viral replication and is relatively highly conserved among serotypes, making it a potential target for diagnostic...

The role of the virology laboratory in the management of hepatitis C virus infection.

Published in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques.

Published in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

Influenza (Flu) and respiratory syncytial virus (RSV) are important viral pathogens that cause lower respiratory tract infections and severe exacerbations of asthma. Molecular biological techniques are permitting a rapid and accurate diagnosis of infections caused by respiratory pathogens, and have typically been applied to upper respiratory sample...

Molecular analysis of cerebrospinal fluid in viral diseases of the central nervous system.

Published in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

The use of nucleic acid (NA) amplification techniques has transformed the diagnosis of viral infections of the central nervous system (CNS). Because of their enhanced sensitivity, these methods enable detection of even low amounts of viral genomes in cerebrospinal fluid. Following more than 10 years of experience, the polymerase chain reaction or o...

Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement.

Published in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

The antiviral effect of anti-influenza drugs such as zanamivir may be demonstrated in patients as an increased rate of decline in viral load over a time course of treatment as compared with placebo. Historically this was measured using plaque assays, or Culture Enhanced Enzyme Linked Immunosorbent Assay (CE-ELISA). to develop and characterise real ...