Erny, Guillaume L Bergström, Edmund T Goodall, David M
Published in
Journal of Chromatography A
This paper provides experimental validation of the use of the Haarhoff-Van der Linde (HVL) peak fitting function to fit experimental capillary zone electrophoresis (CZE) electropherograms. The test mixtures were composed of paraquat over a five order of magnitude concentration range (1.2 microM to 120 mM) and 4-aminopyridine at constant concentrati...
W.M.A Niessen
Published in
Journal of Chromatography A
Abstract In the past few years, the instrumental developments in the field of combined liquid chromatography–mass spectrometry (LC–MS) and related liquid-introduction techniques has been extremely fast. Soon after the demonstration of the ability to obtain multiply-charged ions from proteins by electrospray ionization, a major impetus was given to ...
Lon Hall
Published in
Journal of Chromatography A
Abstract A capillary gas chromatographic method was developed for the analysis of the degradation product pivalic acid in dipivefrin-containing ophthalmic solutions. The linear calibration range was 1.02 to 102 μg/ml ( r = 0.999) and recoveries were greater than 98%. The relative standard deviation was less than 4.2%. The method can be used to accu...
C.E.M. Heeremans A.M. Stijnen R.A.M. Van Der Hoeven W.M.A. Niessen M. Danhof J. van der Greef
Published in
Journal of Chromatography A
Abstract An unknown heptabarbital metabolite, observed in the liquid chromatogram of rat plasma and urine samples after administration of heptabarbital, was identified by liquid chromatography-thermospray tandem mass spectrometry. By applying the parent scan mode for screening and the daughter scan mode for structure elucidation, the metabolite was...
E.R. Verheij U.R. Tjaden W.M.A. Niessen J. Van der Greef
Published in
Journal of Chromatography A
Abstract Pseudo-electrochromatography (pEC) is a separation method that is based on the combination of the chromatographic and electrophoretic behaviour of compounds. This combination enables tuning of the selectivity without changing the composition of the mobile phase. The loadability of pEC is considerably higher than for capillary electrophores...
W.M.A. Niessen P.J.M. Bergers U.R. Tjaden J. van der Greef
Published in
Journal of Chromatography A
Abstract One of the problems of the application of supercritical fluid chromatography ( SFC) in bioanalysis is the fact that many sample pretreatment procedures deliver the solutes of interest in a polar solvent, which upon injection will dramatically disturb the phase system characteristics of the SFC system. The phase-system switching approach, r...
C.E. Werkhoven-Goewie W.M.A. Niessen U.A.Th. Brinkman R.W. Frei
Published in
Journal of Chromatography A
Abstract A method is described for the post-column reaction detection of organosulphur compounds in liquid chromatography, which is based on a ligand-exchange reaction between the palladium(II)-calcein complex and the sulphur-containing compounds. The release of free calcein provides an indirect measure of the amount of organosulphur compounds via ...
