Liquid chromatography (LC) coupled with tandem mass spectrometry (MS-MS) in selected reaction monitoring mode (SRM) has become a widely used technique for the quantification of protein biomarkers in plasma and has already proven to give similar results compared to the conventional immunoassays. To improve the lack of insufficient sensitivity for qu...
Published in Journal of Chromatography A
A specific and sensitive LC–MS/MS method was developed for quantitative determination of closantel in bovine and ovine colostrum and tank milk. Sample preparation consisted of extracting milk samples with acetonitrile/acetone (80/20, v/v) followed by SPE clean-up with Oasis® mixed anion exchange columns. After elution with 5% formic acid in acetoni...
Published in Journal of Chromatography A
When determining endogenous compounds in biological samples, the lack of blank or analyte-free matrix samples involves the use of alternative strategies for calibration and quantitation. This article deals with the development, optimization and validation of a high performance liquid chromatography method for the determination of retinoic acid in p...
Published in Journal of Chromatography A
In this paper, surfactant-coated multi-walled carbon nanotubes (SC-MWNTs) have been proposed as a novel pseudostationary phase (PSP) to enhance the separation of isoquinoline alkaloids in nonaqueous capillary electrophoresis (NACE). Several parameters affecting NACE separation were studied including the MWNT concentration, the electrolyte concentra...
Published in Journal of Chromatography A
The previously published method allowing the separation of non-ferric (iron-free) and ferric (iron-saturated) forms of human serum transferrin via capillary electrophoresis has been further developed. Using a surface response methodology and a three-factorial Doehlert design we have established a new optimized running buffer composition: 50mM Tris–...
Published in Journal of Chromatography A
While numerous analytical methods for phytosterols have been reported, the similar polarity and large molecules of phytosterol esters have made the methods lengthy and complicated. For this reason, an analytical method that could completely separate phytosterol esters including the higher fatty acids such as palmitic acid, stearic acid, oleic acid,...
Published in Journal of Chromatography A
A new operating method, namely three-port operation in a three-zone simulated moving bed (TT-SMB), was developed to improve the separation performance of simulated moving bed (SMB) chromatography. In the TT-SMB, the switching period consists of two steps; extract outlet port is closed at the first step for raffinate production and raffinate outlet ...
Published in Journal of Chromatography A
The ability of asymmetrical flow field-flow fractionation (AF4) coupled online with multiangle light scattering (MALS) and refractive index detector (RI) (AF4-MALS–RI) for monitoring of change in molecular conformation of wheat and barley starch during germination process was evaluated. AF4 provides separation of starch molecules based on their hyd...
Published in Journal of Chromatography A
Chromatin released from dead host cells during in vitro production of IgG monoclonal antibodies exists mostly in complex hetero-aggregates consisting of nucleosomal arrays (DNA+histone proteins), non-histone proteins, and aberrant forms of IgG. They bind immobilized protein A more aggressively than IgG, through their nucleosomal histone components,...
Published in Journal of Chromatography A
Comprehensive on-line two-dimensional liquid chromatography (on-line LC×LC) was used for the characterization of bio-oils obtained by fast pyrolysis of lignocellulosic biomass. The resulting bio-oil contains a large number of oxygenated chemical families and must therefore be upgraded before being used as drop-in transportation biofuels. The good k...
