Ayadi, Dorra Zouari Chouayekh, Hichem Mhiri, Sonda Zerria, Khaled Fathallah, Dahmani M Bejar, Samir
Published in
Journal of biomedicine & biotechnology
We already reported the use of a long synthetic signal peptide (LSSP) to secrete the Streptomyces sp. TO1 amylase by Streptomyces lividans strain. We herein report the expression and secretion of the rat CD11b A-domain using the same LSSP and S. lividans as host strain. We have used the Escherichia coli/Streptomyces shuttle vector pIJ699 for the cl...
Sangpairoj, Kant Changklungmoa, Narin Vanichviriyakit, Rapeepun Sobhon, Prasert Chaithirayanon, Kulathida
Published in
Experimental Parasitology
2-Cys peroxiredoxin (Prx) is the main antioxidant enzyme in Fasciola species for detoxifying hydrogen peroxide which is generated from the hosts’ immune effector cells and the parasites’ own metabolism. In this study, the recombinant Prx protein from Fasciola gigantica (rFgPrx-2) was expressed and purified in a prokaryotic expression system. This r...
Hildebrandt, Niko Wegner, K. David Algar, W. Russ
Published in
Coordination Chemistry Reviews
Optical quantification of several biomarkers at very low concentrations and nanometric distances has become an important requirement for many biosensing applications. Förster resonance energy transfer (FRET) and, in particular, luminescent terbium complex (LTC)-based FRET, is a valuable tool for sensitive and versatile multiplexed FRET. Here, we re...
Duan, Yinong Gu, Xijuan Zhu, Dandan Sun, Wei Chen, Jinling Feng, Jinrong Song, Ke Xu, Feifan He, Xue He, Xingxin
...
Published in
International Journal for Parasitology
Hepatic stellate cells play a key role in the development of hepatic fibrosis. Activated hepatic stellate cells can be reversed to a quiescent-like state or apoptosis can be induced to reverse fibrosis. Some studies have recently shown that Schistosoma mansoni eggs could suppress the activation of hepatic stellate cells and that soluble egg antigen...
Andrewartha, Sarah J. Tazawa, Hiroshi Burggren, Warren W.
Published in
Respiratory Physiology & Neurobiology
The regulation of blood acid–base balance and hematology in day 15 chicken embryos in response to partial water submersion (with egg's air cell in air) and complete submersion producing severe intrinsic hypercapnic hypoxia and recovery in air was studied. The acid–base disturbance during submersion was characterized by initial rapid respiratory cha...
Sidney, John Peters, Bjoern Frahm, Nicole Brander, Christian Sette, Alessandro
Published in
BMC Immunology
BackgroundClass I major histocompatibility complex (MHC) molecules bind, and present to T cells, short peptides derived from intracellular processing of proteins. The peptide repertoire of a specific molecule is to a large extent determined by the molecular structure accommodating so-called main anchor positions of the presented peptide. These rece...
Wang, Ling-Fei Wang, Zhi-Peng Liu, Xiao-Yan Chi, Zhen-Ming
Published in
Bioprocess and Biosystems Engineering
In this study, citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 was investigated. After the compositions of the extract of Jerusalem artichoke tubers for citric acid production were optimized, the results showed that natural components of extract of Jerusalem articho...
Kiuru-Enari, Sari Haltia, Matti
Published in
Handbook of Clinical Neurology
Hereditary gelsolin amyloidosis (HGA) is an autosomally dominantly inherited form of systemic amyloidosis, characterized mainly by cranial and sensory peripheral neuropathy, corneal lattice dystrophy, and cutis laxa. HGA, originally reported from Finland and now increasingly from other countries in Europe, North and South America, and Asia, may sti...
Liu, Yang Yu, Yang Schachner, Melitta Zhao, Weijiang
Published in
Biochemical and Biophysical Research Communications
Neuregulin 1 (Nrg1) functions in neuronal migration, survival and differentiation as well as synaptogenesis during ontogenetic development and maintenance of synaptic functions in the adult mammalian brain. The neural adhesion molecule L1 (L1CAM) functions in similar overlapping, but also non-overlapping roles in the nervous system. In the present ...
You, Jingjing Willcox, Mark D. Madigan, Michele C. Wasinger, Valerie Schiller, Belinda Walsh, Bradley J. Graham, Peter H. Kearsley, John H. Li, Yong
Published in
Advances in Clinical Chemistry
The tear film covers and protects the ocular surface. It contains various molecules including a large variety of proteins. The protein composition of the tear fluid can change with respect to various local and systemic diseases. Prior to the advent of the proteomic era, tear protein analysis was limited to a few analytical techniques, the most comm...
