Granados, Alex Eduardo Alvino Kawai, Kiyoshi
Published in
Journal of Applied Glycoscience
The browning, gelatinization of starch, water sorption, glass transition, and caking properties of freeze-dried maca ( Lepidium meyenii Walpers) powders were investigated and compared with a commercial maca powder. The freeze-dried maca powders had lower optical density (browning) and higher enthalpy change for starch gelatinization than the commer...
Tanaka, Tomonari Matsuura, Ayane Aso, Yuji Ohara, Hitomi
Published in
Journal of Applied Glycoscience
Glycopolymers have attracted increased attention as functional polymeric materials, and simple methods for synthesizing glycopolymers remain needed. This paper reports the aqueous one-pot and chemoenzymatic synthesis of four types of glycopolymers via two reactions: the β-galactosidase-catalyzed glycomonomer synthesis using 4,6-dimetoxy triazinyl β...
Hosaka, Hiroki Shirai, Sayaka Fujita, Sora Tashiro, Mitsuru Hirano, Takako Hakamata, Wataru Nishio, Toshiyuki
Published in
Journal of Applied Glycoscience
Utilizing transglycosylation reaction catalyzed by β- N -acetylhexosaminidase of Stenotrophomonas maltophilia , β-D-fructofuranosyl-(2↔1)-α- N , N ´diacetylchitobioside (GlcNAc 2 -Fru) was synthesized from N -acetylsucrosamine and N , N ´-diacetylchitobiose (GlcNAc 2 ), and β-D-fructofuranosyl-(2↔1)-α- N , N ´, N ´´-triacetylchitotrioside (GlcNAc 3...
Umekawa, Midori Hamada, Kaito Isono, Naoto Karita, Shuichi
Published in
Journal of Applied Glycoscience
Hexokinases catalyze glucose phosphorylation at the first step in glycolysis in eukaryotes. In the budding yeast Saccharomyces cerevisiae , three enzymes for glucose phosphorylation have long been known: Hxk1, Hxk2, and Glk1. In this study, we focus on Emi2, a previously uncharacterized hexokinase-like protein of S. cerevisiae . Our data show that ...
Furushiro, Yuya Kobayashi, Takashi
Published in
Journal of applied glycoscience
Glucose and fructose were treated in subcritical water in the presence of alkali or alkaline earth metal chlorides. All salts accelerated the conversion of saccharides, and alkaline earth metal chloride greatly promoted the isomerization of glucose to fructose. In contrast, alkali metal salts only slightly promoted this isomerization and facilitate...
Ishiyama, Hironobu Yanagita, Ryo C. Takemoto, Kazune Kitaguchi, Natsumi Uezato, Yuuki Sugiyama, Yasunori Sato, Masashi Kawanami, Yasuhiro
Published in
Journal of Applied Glycoscience
D-Allose (D-All), a C-3 epimer of D-glucose (D-Glc), is a naturally rare monosaccharide, which shows anti-proliferative activity against several human cancer cell lines. Unlike conventional anticancer drugs, D-All targets glucose metabolism and is non-toxic to normal cells. Therefore, it has attracted attention as a unique “seed” compound for antic...
Yamaguchi, Sora Sunagawa, Naoki Tachioka, Mikako Igarashi, Kiyohiko Samejima, Masahiro
Published in
Journal of Applied Glycoscience
Thermal inactivation of saccharifying enzymes is a crucial issue for the efficient utilization of cellulosic biomass as a renewable resource. Cellobiohydrolases (CBHs) are a kind of cellulase. In general, CBHs belonging to glycoside hydrolase (GH) family 6 (Cel6) act synergistically with CBHs of GH family 7 (Cel7) and other carbohydrate-active enzy...
Fujii, Takahiro Igarashi, Kiyohiko Samejima, Masahiro
Published in
Journal of Applied Glycoscience
Pyranose 2-oxidases catalyze the oxidation of various pyranose sugars at the C2 position. However, their potential application for detecting sugars other than glucose in blood is hindered by relatively high activity towards glucose. In this study, in order to find a mutant enzyme with enhanced specificity for 1,5-anhydro-D-glucitol (1,5-AG), which ...
Tautau, Feunai Agape Papalii Izumi, Minoru Matsunaga, Emiko Higuchi, Yujiro Takegawa, Kaoru
Published in
Journal of Applied Glycoscience
α-L-Rhamnosidases (α-L-Rha-ases, EC 3.2.1.40) are glycosyl hydrolases (GHs) that hydrolyze a terminal α-linked L-rhamnose residue from a wide spectrum of substrates such as heteropolysaccharides, glycosylated proteins, and natural flavonoids. As a result, they are considered catalysts of interest for various biotechnological applications. α-L-rhamn...
Suzuki, Daisuke Sato, Yuko Kamasaka, Hiroshi Kuriki, Takashi
Published in
Journal of Applied Glycoscience
Phosphoryl oligosaccharides of calcium (POs-Ca) is a calcium salt of phosphoryl maltooligosaccharides made from potato starch. POs-Ca is highly water-soluble and can supply both the calcium ion and acidic oligosaccharides in an aqueous solution. In this study, we investigated the effects of POs-Ca on the mycelial growth and fruiting body yield of P...
