Le, Trinh Ngoc Dang Matsumoto, Akane Kawai, Kiyoshi
Published in
Journal of applied glycoscience
Wheat flour-based batter containing 0 to 20 % trehalose was deep-fried, dried and held in various water activity (a w) conditions. The effects of trehalose content and a w on oil content, water sorption, isothermal mechanical relaxation, and fracture properties were investigated. For comparison, the fracture properties of freeze-dried porous waxy c...
Aizawa, Kenta Takagi, Hiroki Kokubo, Eri Takada, Masayasu
Published in
Journal of applied glycoscience
Carbohydrate materials that produce lower postprandial blood glucose increase are required for diabetic patients. To develop slowly digestible carbohydrates, the effect of degree of polymerization (DP) of α-1,6 glucan on its digestibility was investigated in vitro and in vivo. We prepared four fractions of α-1,6 glucan composed primarily of DP 3-9,...
Yamagishi, Kenji Ike, Masakazu Gau, Mitsuru Tokuyasu, Ken
Published in
Journal of applied glycoscience
Erianthus arundinaceus (ER) is greatly appreciated among domestic energy crops in Japan for the production of fermentable sugars from lignocellulosic polysaccharides. In this study, we developed an efficient Ca(OH)2-based pretreatment of both stems and leaves of ER at ambient temperature with the addition of a washing step for enzymatic saccharific...
Sasaki, Yuki Uchimura, Yumi Kitahara, Kanefumi Fujita, Kiyotaka
Published in
Journal of applied glycoscience
We recently characterized a 3-O-α-D-galactosyl-α-L-arabinofuranosidase (GAfase) for the release of α-D-Gal-(1→3)-L-Ara from gum arabic arabinogalactan protein (AGP) in Bifidobacterium longum subsp. longum JCM7052. In the present study, we cloned and characterized a neighboring α-galactosidase gene (BLGA_00330; blAga3). It contained an Open Reading ...
Yatsuhashi, Hiroko Furuyashiki, Takashi Vo, Phuong Hong Thi Kamasaka, Hiroshi Kuriki, Takashi
Published in
Journal of applied glycoscience
Glycogen is a highly branched storage polysaccharide found mainly in the liver and the muscles. Glycogen is also present in the skin, but its functional role is poorly understood. Recently, it has been reported that glycogen plays an important role in intracellular signal transduction. In the epidermis of the skin, keratinocytes are the predominant...
Teshigahara, Yu Kakizaki, Ikuko Hirao, Wataru Tanaka, Kanji Takahashi, Ryoki
Published in
Journal of Applied Glycoscience
Human urinary trypsin inhibitor (UTI) is a proteoglycan composed of one core protein covalently linked to one glycosaminoglycan, which is a low sulfated chondroitin 4-sulfate. It is used as an anti-inflammatory medicine based on the protease inhibitory activity of the core protein. However, functions of the chondroitin sulfate have not been clarifi...
Ida, Tamami Crofts, Naoko Miura, Satoko Matsushima, Ryo Fujita, Naoko
Published in
Journal of applied glycoscience
Starch biosynthetic enzymes form multi-protein complexes consisting of starch synthase (SS) I, SSIIa, and starch branching enzyme (BE) IIb, which synthesize amylopectin clusters. This study analyzed the starch properties in two double mutant rice lines lacking SSIIa and BEIIb, one of which expressed an inactive BEIIb protein. The ss2a be2b lines sh...
Katoh, Toshihiko Yamamoto, Kenji
Published in
Journal of Applied Glycoscience
Most functional biopharmaceuticals such as antibodies are glycoproteins carrying N -linked oligosaccharides ( N -glycans). In animal cells, these glycans are generally expressed as heterogeneous glycoforms that are difficult to separate into a pure form. The structure of these glycans directly affects several biological aspects of the glycoproteins...
Nakamura, Akihiko Kanazawa, Takashi Furuta, Tadaomi Sakurai, Minoru Saloheimo, Markku Samejima, Masahiro Koivula, Anu Igarashi, Kiyohiko
Published in
Journal of Applied Glycoscience
Cellobiohydrolase I from Trichoderma reesei ( Tr Cel7A) is one of the best-studied cellulases, exhibiting high activity towards crystalline cellulose. Tryptophan residues at subsites -7 and -4 (Trp40 and Trp38 respectively) are located at the entrance and middle of the tunnel-like active site of Tr Cel7A, and are conserved among the GH family 7 cel...
Koh, Sangho Mizuno, Masahiro Izuoka, Yuto Fujino, Naoto Hamada-Sato, Naoko Amano, Yoshihiko
Published in
Journal of Applied Glycoscience
The genus Pestalotiopsis are endophytic fungi that have recently been identified as cellulolytic system producers. We herein cloned a gene coding for a xylanase belonging to glycoside hydrolase (GH) family 10 ( Pes Xyn10A) from Pestalotiopsis sp. AN-7, which was isolated from the soil of a mangrove forest. This protein was heterologously expressed ...
