Haraguchi, Ryuma Kitazawa, Riko Imai, Yuuki Kitazawa, Sohei
Published in
Histochemistry and cell biology
Longitudinal bone growth progresses by continuous bone replacement of epiphyseal cartilaginous tissue, known as "growth plate", produced by columnar proliferated- and differentiated-epiphyseal chondrocytes. The endochondral ossification process at the growth plate is governed by paracrine signals secreted from terminally differentiated chondrocytes...
Komori, Toshihisa
Published in
Histochemistry and cell biology
Runx2 is a transcription factor that is essential for osteoblast differentiation and chondrocyte maturation. Ihh, expressed in prehypertrophic and hypertrophic chondrocytes, is required for the specification of Runx2+ osteoprogenitors in endochondral bone development. Runx2 induces Sp7, an essential transcription factor for osteoblast differentiati...
Ono, Takehito Nakashima, Tomoki
Published in
Histochemistry and cell biology
The bone is an essential organ for locomotion and protection of the body, as well as hematopoiesis and mineral homeostasis. In order to exert these functions throughout life, bone tissue undergoes a repeating cycle of osteoclastic bone resorption and osteoblastic bone formation. The osteoclast is a large, multinucleated cell that is differentiated ...
Kitazawa, Riko Haraguchi, Ryuma Fukushima, Mana Kitazawa, Sohei
Published in
Histochemistry and cell biology
Hard tissue homeostasis is regulated by the balance between bone formation by osteoblasts and bone resorption by osteoclasts. This physiologic process allows adaptation to mechanical loading and calcium homeostasis. Under pathologic conditions, however, this process is ill-balanced resulting in either over-resorption or over-formation of hard tissu...
Mizuno, Hiroki Kikuta, Junichi Ishii, Masaru
Published in
Histochemistry and cell biology
There are as many as 200 cell types in the body, and highly sophisticated and varied life phenomena are carried out by cell migration to appropriate places at appropriate times following the appropriate interactions. Recent advances in optical imaging technology using multi-photon excitation microscopy have enabled visualization inside intact bone ...
Hasegawa, Tomoka
Published in
Histochemistry and cell biology
Bone mineralization is initiated by matrix vesicles, small extracellular vesicles secreted by osteoblasts, inducing the nucleation and subsequent growth of calcium phosphate crystals inside. Although calcium ions (Ca2+) are abundant throughout the tissue fluid close to the matrix vesicles, the influx of phosphate ions (PO43-) into matrix vesicles i...
Liu, Hongrui Li, Dongfang Liu, Shanshan Liu, Zhaopeng Li, Minqi
Published in
Histochemistry and cell biology
Insulin-like growth factor 2 (IGF2) messenger RNA-binding proteins (IMPs) are a family of oncofetal RNA-binding proteins that play important roles in cell migration, renewal, and metabolism. IMP2 gene expression may be important in determining IGF2 levels and might, thereby, be central to bone metabolism. In our present study, IMP2-deficient mice e...
Yamamoto, Yutaro Makino, Takamitsu Kudo, Hideo Ihn, Hironobu Murakami, Yasuko Matsufuji, Senya Fujiwara, Kunio Shin, Masashi
Published in
Histochemistry and cell biology
No expression and distribution patterns of polyamines (PAs), spermine, spermidine, and their precursor putrescine in mammalian hair follicle are available, although polyamines are known to correlate well with hair growth and epidermal tumor genesis. Immunohistochemistry (IHC) using our original two monoclonal antibodies (mAbs) ASPM-29 specific for ...
Ansari, Sabiha M Saquib, Quaiser Attia, Sabry M Abdel-Salam, Eslam M Alwathnani, Hend A Faisal, Mohammad Alatar, Abdulrahman A Al-Khedhairy, Abdulaziz A Musarrat, Javed
Published in
Histochemistry and cell biology
Pendimethalin (PM) is a dinitroaniline herbicide extensively applied against the annual grasses and broad-leaved weeds. There is no report available on PM-induced low-dose genotoxicity in human primary cells and in vivo test models. Such data gap has prompted us to evaluate the genotoxic potential of PM in human lymphocytes and rats. PM selectively...
Pastorek, Lukáš Venit, Tomáš Hozák, Pavel
Published in
Histochemistry and cell biology
Artifact-free microscopic images represent a key requirement of multi-parametric image analysis in modern biomedical research. Holography microscopy (HM) is one of the quantitative phase imaging techniques, which has been finding new applications in life science, especially in morphological screening, cell migration, and cancer research. Rather tha...
