Published in Gan
The kinetics of nitrosation of thioproline was studied. The rate of the reaction increased with decrease in pH, and was first-order with respect to nitrite concentration. The reaction rate was proportional to the concentration of total thioproline (free plus protonated), not to that of free thioproline. The initial reaction rate followed the equati...
Published in Gan
DNAs were isolated from about 30 species of animals and digested with EcoRI. DNA fragments of about 20-30 kilobase pairs from pig and wild boar cells hybridized with a probe for the pol gene of human T-cell leukemia virus type I.
Published in Gan
The promoting effects of 5 bile acids on liver carcinogenesis were investigated in male Fischer rats initially treated with diethylnitrosamine (DEN). Two weeks after a single dose of DEN (200 mg/kg, intraperitoneally), rats were given bile acids for 8 weeks. At 3 weeks following DEN administration, all rats were subjected to partial hepatectomy. Am...
Published in Gan
When human alveolar macrophages (AM) obtained by lavage of the lungs of healthy donors were incubated in medium with or without lipopolysaccharide (LPS) they released a factor(s) with tumor cell killing activity. This tumor cytolytic and/or cytotoxic factor(s) (TCF) was assayed by measuring its effect in inhibiting target cell growth. TCF activity ...
Published in Gan
The effect of crude stomach extracts (CSE) on X-ray-induced gastric tumorigenesis was examined. ICR mice were treated with two or four administrations of CSE at one-week intervals then irradiated with 20 Gy of X-rays one week after the final CSE administration. Unexpectedly, the incidence of X-ray-induced tumors was not significantly altered by two...
Published in Gan
A new water-soluble nitrosourea derivative, methyl 6-[3-(2-chloroethyl)-3-nitrosoureido]-6-deoxy-alpha-D-glucopyranoside (MCNU), was tested for antitumor activity against murine tumors (L1210 and P388 leukemias, B16 melanoma, and Lewis lung carcinoma) and BC-47 rat bladder carcinoma, and the results were compared with those for four other nitrosour...
Published in Gan
Three olivoretins, A, B and C (isolated from Streptoverticillium olivoreticuli), which are O-methylated teleocidin B isomers, were found to be biologically inactive. A fourth olivoretin, D, which has a free hydroxyl group and is identical to one of the four teleocidin B isomers, teleocidin B-4 (teleocidin B of Hirata) was biologically active. These...
Published in Gan
The potential carcinogenicity of captafol in B6C3F1 mice was examined. Captafol was given at levels of 0 (control), 0.075, 0.15 or 0.3% in the diet to a total of 203 males and 203 females for 96 weeks, after which time the animals were returned to basal diet for a further 8 weeks. Mice surviving 42 weeks or longer were included in the effective num...
Published in Gan
Alkylehter and ester derivatives of the antitumor cyclic hexapeptide RA-V obtained from the roots of Rubia cordifolia (Rubiaceae) were synthesized and bioassayed for activity against cultured tumor cells. RA-V and its n-hexylether showed significant effects against human nasopharynx carcinoma (KB), P388 lymphocytic leukemia and MM2 mammary carcinom...
Published in Gan
Synthetic analogues (indolactams) related to the tumor promoter teleocidin were synthesized chemically. Of four indolactam-Vs lacking the monoterpenoid moiety of native teleocidin, (-)-indolactam-V bound to the 12-O-tetradecanoylphorbol-13-acetate receptor in cell membranes and induced both adhesion of HL-60 cells and ornithine decarboxylase activi...
