Tanahashi, Ryoya Afiah, Tira Siti Nur Nishimura, Akira Watanabe, Daisuke Takagi, Hiroshi
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FEMS yeast research
Ubiquitination is a key signal for endocytosis of proteins on the plasma membrane. The ubiquitin ligase Rsp5 of Saccharomyces cerevisiae, which contains an amino-terminal membrane-binding C2 domain, three substrate-recognizing tryptophan-tryptophan (WW) domains and a carboxyl-terminal catalytic homologous to the E6-AP carboxyl terminus (HECT) domai...
Hoshida, Hisashi Kagawa, Shota Ogami, Kentaro Akada, Rinji
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FEMS yeast research
Kluyveromyces marxianus is a thermotolerant, ethanol-producing yeast that requires oxygen for efficient ethanol fermentation. Under anaerobic conditions, glucose consumption and ethanol production are retarded, suggesting that oxygen affects the metabolic state of K. marxianus. Mitochondria require oxygen to function, and their forms and number var...
Fauzee, Yasmin Nabilah Binti Mohd Taniguchi, Naoki Ishiwata-Kimata, Yuki Takagi, Hiroshi Kimata, Yukio
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FEMS yeast research
Dysfunction or capacity shortage of the endoplasmic reticulum (ER) is cumulatively called ER stress and provokes the unfolded protein response (UPR). In various yeast species, the ER-located transmembrane protein Ire1 is activated upon ER stress and performs the splicing reaction of HAC1 mRNA, the mature form of which is translated into a transcrip...
Shekhawat, Kirti Bauer, Florian F Setati, Mathabatha E
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FEMS yeast research
The yeast Lachancea thermotolerans is of significant biotechnological interest, and selected strains of this species have become commonly used starter cultures in wine fermentation. However, the impact of this species on wine is frequently limited by the rapid dominance of Saccharomyces cerevisiae strains which are better adapted to wine alcoholic ...
Soberanes-Gutiérrez, Cinthia V León-Ramírez, Claudia Sánchez-Segura, Lino Cordero-Martínez, Emmanuel Vega-Arreguín, Julio C Ruiz-Herrera, José
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FEMS yeast research
Ustilago maydis is a Basidiomycota fungus, in which very little is known about its mechanisms of cell survival and death. To date, only the role of metacaspase1, acetate and hydrogen peroxide as inducers of cell death has been investigated. In the present work, we analyzed the lifespan of U. maydis compared with other species like Sporisorium reili...
Erian, Anna Maria Egermeier, Michael Rassinger, Alice Marx, Hans Sauer, Michael
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FEMS yeast research
Yarrowia lipolytica is a yeast with many talents, one of them being the production of citric acid. Although the citrate biosynthesis is well studied, little is known about the transport mechanism by which citrate is exported. To gain better insight into this mechanism, we set out to identify a transporter involved in citrate export of Y. lipolytica...
Onetto, Cristobal A Schmidt, Simon A Roach, Michael J Borneman, Anthony R
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FEMS yeast research
Aureobasidium pullulans is the most abundant and ubiquitous species within the genus and is also considered a core component of the grape juice microflora. So far, a small number of other Aureobasidium species have been reported, that in contrast to A. pullulans, appear far more constrained to specific habitats. It is unknown whether grape juice is...
Liu, Z Lewis Huang, Xiaoqiu
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FEMS yeast research
The adapted industrial yeast strain Saccharomyces cerevisiae NRRL Y-50049 is able to in situ detoxify major toxic aldehyde compounds derived from sugar conversion of lignocellulosic biomass while producing ethanol. Pathway-based studies on its mechanisms of tolerance have been reported previously, however, little is known about transposable element...
Shahi, Garima Kumar, Mohit Kumari, Sonam Rudramurthy, Shivaprakash M Chakrabarti, Arunaloke Gaur, Naseem A Singh, Ashutosh Prasad, Rajendra
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FEMS yeast research
The present study is an attempt to determine the lipid composition of Candida auris and to highlight if the changes in lipids can be correlated to high drug resistance encountered in C. auris. For this, the comparative lipidomics landscape between drug-susceptible (CBS10913T) and a resistant hospital isolate (NCCPF_470033) of C. auris was determine...
Myburgh, Marthinus W Rose, Shaunita H Viljoen-Bloom, Marinda
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FEMS yeast research
Bioethanol production from starchy biomass via consolidated bioprocessing (CBP) will benefit from amylolytic Saccharomyces cerevisiae strains that produce high levels of recombinant amylases. This could be achieved by using strong promoters and modification thereof to improve gene expression under industrial conditions. This study evaluated eight e...