Nizovoy, Paula Bellora, Nicolás Haridas, Sajeet Sun, Hui Daum, Chris Barry, Kerrie Grigoriev, Igor V Libkind, Diego Connell, Laurie B Moliné, Martín
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FEMS yeast research
Cold environments impose challenges to organisms. Polyextremophile microorganisms can survive in these conditions thanks to an array of counteracting mechanisms. Naganishia vishniacii, a yeast species hitherto only isolated from McMurdo Dry Valleys, Antarctica, is an example of a polyextremophile. Here we present the first draft genomic sequence of...
Nagamatsu, Sheila Tiemi Coutouné, Natalia José, Juliana Fiamenghi, Mateus Bernabe Pereira, Gonçalo Amarante Guimarães Oliveira, Juliana Velasco de Castro Carazzolle, Marcelo Falsarella
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FEMS yeast research
Ethanol production has key differences between the two largest producing countries of this biofuel, Brazil and the USA, such as feedstock source, sugar concentration and ethanol titers in industrial fermentation. Therefore, it is highly probable that these specificities have led to genome adaptation of the Saccharomyces cerevisiae strains employed ...
Tulha, Joana Amorim-Rodrigues, Mariana Esquembre, Lidia Alejo Rauch, Sebastien Tamás, Markus J Lucas, Cândida
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FEMS yeast research
The Saccharomyces cerevisiae MBOAT O-acyltransferase Gup1 is involved in many processes, including cell wall and membrane composition and integrity, and acetic acid-induced cell death. Gup1 was previously shown to interact physically with the mitochondrial membrane VDAC (Voltage-Dependent Anion Channel) protein Por1 and the ammonium transceptor Mep...
Nijland, Jeroen G Shin, Hyun Yong Dore, Eleonora Rudinatha, Donny de Waal, Paul P Driessen, Arnold J M
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FEMS yeast research
Co-consumption of D-xylose and D-glucose by Saccharomyces cerevisiae is essential for cost-efficient cellulosic bioethanol production. There is a need for improved sugar conversion rates to minimize fermentation times. Previously, we have employed evolutionary engineering to enhance D-xylose transport and metabolism in the presence of D-glucose in ...
Schmidl, Sina Iancu, Cristina V Reifenrath, Mara Choe, Jun-Yong Oreb, Mislav
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FEMS yeast research
Glucose uptake assays commonly rely on the isotope-labeled sugar, which is associated with radioactive waste and exposure of the experimenter to radiation. Here, we show that the rapid decrease of the cytosolic pH after a glucose pulse to starved Saccharomyces cerevisiae cells is dependent on the rate of sugar uptake and can be used to determine th...
Fathi, Zahra Tramontin, Larissa Ribeiro Ramos Ebrahimipour, Gholamhossein Borodina, Irina Darvishi, Farshad
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FEMS yeast research
β-Carotene is a yellow-orange-red pigment used in food, cosmetics and pharmacy. There is no commercial yeast-based process for β-carotene manufacturing. In this work, we engineered the baker's yeast Saccharomyces cerevisiae by expression of lipases and carotenogenic genes to enable the production of β-carotene on hydrophobic substrates. First, the ...
Yang, Bai-Xue Xie, Cai-Yun Xia, Zi-Yuan Wu, Ya-Jing Gou, Min Tang, Yue-Qin
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FEMS yeast research
Engineered Saccharomyces cerevisiae can reduce xylose to xylitol. However, in S.cerevisiae, there are several endogenous enzymes including xylitol dehydrogenase encoded by XYL2, sorbitol dehydrogenases encoded by SOR1/SOR2 and xylulokinase encoded by XKS1 may lead to the assimilation of xylitol. In this study, to increase xylitol accumulation, thes...
Sprenger, Marcel Brunke, Sascha Hube, Bernhard Kasper, Lydia
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FEMS yeast research
Although less prevalent than its relative Candida albicans, the yeast Candida glabrata is a successful pathogen of humans which causes life-threatening candidiasis. It is thus vital to understand the pathogenicity mechanisms and contributing genes in C. glabrata. However, gene complementation as a tool for restoring the function of a previously del...
Borkowska, Monika Białas, Wojciech Celińska, Ewelina
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FEMS yeast research
Accurate quantitation of gene expression levels require sensitive, precise and reproducible measurements of specific transcripts. Normalization to a reference gene is the most common practice to minimize the impact of the uncontrolled variation. The fundamental prerequisite for an accurate reference gene is to be stably expressed amongst all the sa...
Tanahashi, Ryoya Afiah, Tira Siti Nur Nishimura, Akira Watanabe, Daisuke Takagi, Hiroshi
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FEMS yeast research
Ubiquitination is a key signal for endocytosis of proteins on the plasma membrane. The ubiquitin ligase Rsp5 of Saccharomyces cerevisiae, which contains an amino-terminal membrane-binding C2 domain, three substrate-recognizing tryptophan-tryptophan (WW) domains and a carboxyl-terminal catalytic homologous to the E6-AP carboxyl terminus (HECT) domai...
