Sauvaget, Maëlle Hutton, Fraser Coull, Robert Vavassori, Stefano Wang, Ke Reznik, Aleksandra Chyker, Tatsiana Newfield, Chelsea G Euston, Eloise Benary, Gerrit
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FEMS yeast research
The five-membered PRS gene family of Saccharomyces cerevisiae is an example of gene duplication allowing the acquisition of novel functions. Each of the five Prs polypeptides is theoretically capable of synthesising PRPP but at least one of the following heterodimers is required for survival: Prs1/Prs3, Prs2/Prs5 and Prs4/Prs5. Prs3 contains a pent...
Kijpornyongpan, Teeratas Urbina, Hector Suh, Sung-Oui Luangsa-Ard, Jennifer Aime, M Catherine Blackwell, Meredith
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FEMS yeast research
Candida tanzawaensis clade members are now placed in Suhomyces. The group was virtually unknown until the early 2000s. Here, we review progress made on Suhomyces over the last two decades and provide data from reports of new members of the group from distant localities worldwide, their habitats and a new study of mating loci that helps explain earl...
Börlin, Christoph S Cvetesic, Nevena Holland, Petter Bergenholm, David Siewers, Verena Lenhard, Boris Nielsen, Jens
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FEMS yeast research
One of the fundamental processes that determine cellular fate is regulation of gene transcription. Understanding these regulatory processes is therefore essential for understanding cellular responses to changes in environmental conditions. At the core promoter, the regulatory region containing the transcription start site (TSS), all inputs regulati...
Krocová, Eliška Neradová, Sylva Kupcik, Rudolf Janovská, Sylva Bílková, Zuzana Heidingsfeld, Olga
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FEMS yeast research
Most of the phosphatases of human fungal pathogens Candida albicans and C. parapsilosis have never been experimentally characterised, although dephosphorylation reactions are central to many biological processes. PHO15 genes of these yeasts have been annotated as the sequences encoding 4-nitrophenyl phosphatase, on the basis of homology to PHO13 ge...
Kumar, Deepak Sharma, Nimisha
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FEMS yeast research
Transcription of protein-coding genes is a highly regulated process. In eukaryotes, it involves cross-talk between the RNA polymerase II enzyme and different proteins of the transcriptional machinery. Twelve different subunits, Rpb1 to Rpb12, constitute RNA polymerase II. The sequence of the Rpb7 subunit is highly conserved across organisms. Howeve...
Sj, Sudharshan Veerabhadrappa, Bhavana Subramaniyan, Subasri Dyavaiah, Madhu
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FEMS yeast research
The budding yeast, Saccharomyces cerevisiae, is an efficient model for studying oxidative stress, programmed cell death and aging. The present study was carried out to investigate antioxidant, the anti-apoptotic and anti-aging activity of a natural compound, astaxanthin, in S. cerevisiae model. The survivability of yeast antioxidant-deficient strai...
Bracher, Jasmine M Martinez-Rodriguez, Oscar A Dekker, Wijb J C Verhoeven, Maarten D van Maris, Antonius J A Pronk, Jack T
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FEMS yeast research
Expression of a heterologous xylose isomerase, deletion of the GRE3 aldose-reductase gene and overexpression of genes encoding xylulokinase (XKS1) and non-oxidative pentose-phosphate-pathway enzymes (RKI1, RPE1, TAL1, TKL1) enables aerobic growth of Saccharomyces cerevisiae on d-xylose. However, literature reports differ on whether anaerobic growth...
Wijsman, Melanie Swiat, Michal A Marques, Wesley L Hettinga, Johanna K van den Broek, Marcel Torre Cortés, Pilar de la Mans, Robert Pronk, Jack T Daran, Jean-Marc Daran-Lapujade, Pascale
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FEMS yeast research
Hexose transporter-deficient yeast strains are valuable testbeds for the study of sugar transport by native and heterologous transporters. In the popular Saccharomyces cerevisiae strain EBY.VW4000, deletion of 21 transporters completely abolished hexose transport. However, repeated use of the LoxP/Cre system in successive deletion rounds also resul...
Giannattasio, Sergio Mazzoni, Cristina
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FEMS yeast research
Cioch-Skoneczny, Monika Satora, Pawel Skotniczny, Magdalena Skoneczny, Szymon
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FEMS yeast research
The aim of the study was to characterise, quantitatively and qualitatively, the yeast microbiota found during spontaneous fermentation of grape musts obtained from the red grape varieties 'Rondo' and 'Regent'. For the research, grapes originating from two vineyards (Srebrna Góra and Spotkaniówka) located in southern Poland were used. Musts of the s...
