Inflammatory Cytokines Promote Clonal Hematopoiesis with Specific Mutations in Ulcerative Colitis Patients.
Published in Experimental hematology
Modest and nonessential roles of the endocannabinoid system in immature hematopoiesis of mice.
...Published in Experimental hematology
Endocannabinoids are lipid mediators that signal via several seven-transmembrane domain G protein-coupled receptors. The endocannabinoid receptor CB2 is expressed on blood cells, including stem cells, and mediates the effects of cannabinoids on the immune system. The role of the endocannabinoid system in immature hematopoiesis is largely elusive. B...
Rats provide a superior model of human stress erythropoiesis.
Published in Experimental hematology
Mouse models are widely used to study human erythropoiesis in vivo. One important caveat using mouse models is that mice often develop significant extramedullary erythropoiesis with anemia, which could mask important phenotypes. To overcome this drawback in mice, here we established in vitro and in vivo rat models for the studies of stress erythrop...
Increased platelet function during frailty.
Published in Experimental hematology
Frailty is highly associated with cardiovascular diseases (CVD) and diabetes mellitus (DM). Aging, CVD, and DM are all associated with an increase in platelet function. Therefore, the aim of this study was to evaluate platelet function during frailty. We selected a total of 37 older adults who were divided into two groups, frail (n = 16) and robust...
Biological implications of clonal hematopoiesis.
Published in Experimental hematology
Adult hematological malignancies, such as acute myeloid leukemia, are thought to arise through the gradual acquisition of oncogenic mutations within long-lived hematopoietic stem cells (HSCs). Genomic analysis of peripheral blood DNA has recently identified leukemia-associated genetic mutations within otherwise healthy individuals, an observation t...
CTLA4Ig-based reduced intensity conditioning and donor lymphocyte infusions for haploidentical transplantation in refrac...
Published in Experimental hematology
CTLA4Ig-primed donor lymphocyte infusions (DLIs) have been found to promote natural killer (NK) cell-mediated anti-leukemia effect following haploidentical hematopoietic cell transplantation (HCT). Incorporation of CTLA4Ig in conditioning aided long-term remission in myeloma probably by blocking the CD28-CD86 pro-survival pathway when combined with...
Transcription factor Oct1 protects against hematopoietic stress and promotes acute myeloid leukemia.
...Published in Experimental hematology
A better understanding of the development and progression of acute myelogenous leukemia (AML) is necessary to improve patient outcome. Here we define roles for the transcription factor Oct1/Pou2f1 in AML and normal hematopoiesis. Inappropriate reactivation of the CDX2 gene is widely observed in leukemia patients and in leukemia mouse models. We sho...
Remission clone in acute myeloid leukemia shows growth advantage after chemotherapy but is distinct from leukemic clone....
...Published in Experimental hematology
In a previously published case study of acute myeloid leukemia, we tracked the dynamics of somatic mutations over 9 years. Interestingly, we observed a group of mutations that expanded during remission, which we named the "remission clone." To determine the nature of the remission clones, we performed flow cytometry-based cell sorting followed by u...
Maintenance and enhancement of human peripheral blood mobilized stem/progenitor cell engraftment after ex vivo culture v...
...Published in Experimental hematology
Currently, there is a growing need for culturing hematopoietic stem/progenitor cells (HSPCs) in vitro for various clinical applications including gene therapy. Compared with cord blood (CB) CD34+ HSPCs, it is more challenging to maintain or expand CD34+ peripheral blood mobilized stem/progenitor cells (PBSCs) ex vivo. To fill this knowledge gap, we...
CD97 expression is associated with poor overall survival in acute myeloid leukemia.
Published in Experimental hematology
CD97, a member of the adhesion G-protein coupled receptor family, is normally expressed on leukocytes and smooth muscles. CD97 is also expressed in a variety of solid cancers, particularly those with aggressive metastatic phenotypes. Here we characterize the clinical significance of CD97 in acute myeloid leukemia (AML). We analyzed 173 patients fro...
