Robles-Garcia, Miguel Thimonier, Chloë Angoura, Konstantina Ozga, Ewa MacPherson, Heather Blin, Guillaume
Published in
Development (Cambridge, England)
Notochord progenitors (NotoPs) represent a scarce yet crucial embryonic cell population, playing important roles in embryo patterning and eventually giving rise to the cells that form and maintain intervertebral discs. The mechanisms regulating NotoPs emergence are unclear. This knowledge gap persists due to the inherent complexity of cell fate pat...
Forbes Beadle, Lauren Sutcliffe, Catherine Ashe, Hilary L
Published in
Development (Cambridge, England)
Live imaging of transcription in the Drosophila embryo using the MS2 or PP7 systems is transforming our understanding of transcriptional regulation. However, insertion of MS2/PP7 stem-loops into endogenous genes requires laborious CRISPR genome editing. Here, we exploit the previously described Minos-mediated integration cassette (MiMIC) transposon...
Ono, Masahiro Crompton, Tessa
Published in
Development (Cambridge, England)
Progenitor cells initiate development upon receiving key signals, dynamically altering gene and protein expression to diverge into various lineages and fates. Despite the use of several experimental approaches, including the Fluorescent Timer-based method Timer-of-cell-kinetics-and-activity (Tocky), analysing time-dependent processes at the single-...
Rosello-Diez, Alberto Menchero, Sergio
Published in
Development (Cambridge, England)
Pinheiro, Amanda Petty, Christopher A Stephens, Chelsea E Cabrera, Kevin Palanques-Tost, Eric Gower, Adam C Marano, Madison Leviss, Ethan M Boberg, Matthew J Mahendran, Jawahar
...
Published in
Development (Cambridge, England)
The coordinate regulation of metabolism and epigenetics to establish cell state-specific gene expression patterns during lineage progression is a central aspect of cell differentiation, but the factors that regulate this elaborate interplay are not well-defined. The imprinted Dlk1-Dio3 noncoding RNA (ncRNA) cluster has been associated with metaboli...
Popiel, Evelyn M Ahluwalia, Rhea Schuetz, Stefan Yu, Bin Derry, W Brent
Published in
Development (Cambridge, England)
The formation and patterning of unicellular biological tubes is essential for metazoan development. It is well established that vascular tubes and neurons use similar guidance cues to direct their development, but the downstream mechanisms that promote the outgrowth of biological tubes are not well characterized. We show that the conserved kinase M...
Yu, Jun Zhang, Yanbo Zhang, Jian Che, Pengfei Long, Gaoxin Yang, Zhuoxuan Ji, Sheng-Jian
Published in
Development (Cambridge, England)
It has been shown that 5-methylcytosine (m5C), one of the most abundant modifications on RNA, regulates various biological processes. However, the function of m5C modification in the nervous system is still largely unknown. Here, we show that the m5C reader Ybx1 is highly expressed in the developing mouse hippocampus in the central nervous system (...
Rumbo, Mireia Alsina, Berta
Published in
Development (Cambridge, England)
Human inner ear organoids are three-dimensional tissular structures grown in vitro that recapitulate some aspects of the fetal inner ear and allow the differentiation of inner ear cell types. These organoids offer a system in which to study human inner ear development, mutations causing hearing loss and vertigo, and new therapeutic drugs. However, ...
Briscoe, James Brown, Katherine
Published in
Development (Cambridge, England)
Landau, Shira Okhovatian, Sargol Zhao, Yimu Liu, Chuan Shakeri, Amid Wang, Ying Ramsay, Kaitlyn Kieda, Jennifer Jiang, Richard Radisic, Milica
...
Published in
Development (Cambridge, England)
This Review explores the rapidly evolving field of bioengineered vasculature, a key area of focus in tissue engineering and regenerative medicine. The broad relevance of this topic is attributed to its impacts on a wide range of biological processes, enabling studies in tissue development, fundamental biology and drug discovery, and the application...