Bassøe, Carl-Fredrik Li, Nianyu Ragheb, Kathy Lawler, Gretchen Sturgis, Jennie Robinson, J Paul
Published in
Cytometry. Part B, Clinical cytometry
The oxidative burst is frequently evaluated by the conversion of dihydrorhodamine 123 (DHR) to rhodamine 123 (R123) and hydroethidium (HE) to ethidium with the use of flow cytometry (FCM). Added R123 accumulates in mitochondria, but during phagocytosis R123 originating from DHR has been observed in neutrophil granules. The present study was designe...
Macey, Marion McCarthy, Desmond Azam, Urooj Milne, Timothy Golledge, Peter Newland, Adrian
Published in
Cytometry. Part B, Clinical cytometry
Ethylenediaminetetraacetic acid (EDTA) is the anticoagulant recommended for full blood counts, citrate is recommended for coagulation and platelet studies, and citrate-theophylline-adenosine-dipyridamole (CTAD) inhibits platelet activation. Because the combination of EDTA and CTAD (E/C) is better than EDTA or CTAD alone for measuring platelet param...
Bleesing, Jack J H Fleisher, Thomas A
Published in
Cytometry. Part B, Clinical cytometry
The B220 isoform in humans is developmentally regulated in humans, tied to the acquisition of a memory phenotype, and as such can be used as a differentiation-specific CD45 isoform, akin to the use of CD45 isoforms to distinguish between naive and memory T-cell subsets. Patients with immunodeficiency disorders, associated with defective memory B-ce...
Riera, Norma Canalejo, Katia Aixalá, Mónica Rosso, Marisa Gaddi, Eduardo Bracco, María M de E de Galassi, Nora
Published in
Cytometry. Part B, Clinical cytometry
Lima, Margarida Pinto, Luísa Dos Anjos Teixeira, Maria Canelhas, Aurea Mota, Alexandra Cabeda, José Manuel Silva, Cidália Queirós, Maria Luís Fonseca, Sónia Santos, Ana Helena
...
Published in
Cytometry. Part B, Clinical cytometry
We report a case of a patient with two B-cell lymphoproliferative disorders: CD5(-)/CD23(+) B-cell chronic lymphocytic leukemia and CD5(+)/CD23(-) mantle cell lymphoma. These disorders were diagnosed simultaneously based on flow cytometry, immunohistochemistry, fluorescence in situ hybridization, and polymerase chain reaction-based molecular studie...
Garcia, Jacinto Duran, Angel Tabernero, Maria Dolores Garcia Plaza, Asunción Flores Corral, Teresa Najera, Maria Luisa Gomez-Alonso, Alberto Orfao, Alberto
Published in
Cytometry. Part B, Clinical cytometry
Numerical abnormalities of chromosomes 17 and 18 were relatively common findings in patients with colorectal cancer, with chromosome 17 being associated with a higher incidence of tumors localized to the rectum and a worse clinical outcome. Cytometry Part B (Clin. Cytometry) 51B:14-20, 2003.
Elghetany, M Tarek Patel, Jyoti Martinez, Joe Schwab, Hanna
Published in
Cytometry. Part B, Clinical cytometry
CD87 is expressed on granulocytes at the band and segmented neutrophil stage of development and can be used to study normal and abnormal granulopoiesis.
Marti, Gerald E Carter, Patricia Abbasi, Fatima Washington, Glennelle C Jain, Nisha Zenger, Vincent E Ishibe, Naoko Goldin, Lynn Fontaine, Laura Weissman, Nancy
...
Published in
Cytometry. Part B, Clinical cytometry
Individual components of BCML and other B-cell abnormalities were observed in almost half of the apparently unaffected individuals. Our findings suggested that BCML may be an early detectable abnormality in BCLL. The spectrum of some of these observed abnormalities suggested the involvement of different B-cell subpopulations or different pathways i...
Newell, James O Cessna, Melissa H Greenwood, Jay Hartung, Leah Bahler, David W
Published in
Cytometry. Part B, Clinical cytometry
This study highlights the importance of CD117 when evaluating acute leukemias by flow cytometry, which was necessary in this case to suggest that the blasts were phenotypically abnormal myeloblasts. In addition, this case presented an unusual acute myeloid leukemia phenotype that will likely be encountered by others and could be difficult to interp...
Vos, Jeffrey A Simurdak, Jerry H Davis, Brad J Myers, Jerome B Brissette, Mark D
Published in
Cytometry. Part B, Clinical cytometry
Disaggregation of tissue for flow cytometric analysis by vortexing appears to provide adequate and representative cellular material. This technique is ideal for inaspirable bone marrows and small biopsies where tissue preservation for histology is paramount.
