The use of erythrocytes as biomarkers with a timeline.
Published in Cytometry. Part B, Clinical cytometry
Quick identification of target antigens by tissue flow cytometry for CAR-T therapy in B-cell malignancies.
...Published in Cytometry. Part B, Clinical cytometry
Use of flow cytometry and cytology to differentiate breast implant-associated anaplastic large cell lymphoma from reacti...
Published in Cytometry. Part B, Clinical cytometry
The combination of cytology and multiparametric flow cytometry (MFC) may be useful in the diagnosis of breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) and may be a practical way to differentiate lymphoma from benign and reactive seromas. Although the Brazilian breast implant market is the second largest in the world, with severa...
Flow cytometric immunophenotyping of plasma cells across the spectrum of plasma cell proliferative disorders: A fresh in...
...Published in Cytometry. Part B, Clinical cytometry
The expression pattern of common antigens including cytoplasmic kappa/lambda ratio (cyKLR) was evaluated by flow cytometric immunophenotyping (FCMI) to explore their relevance in discriminating normal and aberrant plasma cells (NPC and APC, respectively) across spectrum of plasma cell proliferative disorders (PCPD). In this prospective analysis, 79...
Diagnostic utility of flow cytometry based coated-platelets assay as a biomarker to predict thrombotic or hemorrhagic ph...
Published in Cytometry. Part B, Clinical cytometry
Coated-platelets are sub-population of platelets "coated" with highly procoagulant proteins and phosphatidylserine that sustains thrombin generation. They are produced upon dual agonist stimulation by collagen and thrombin. This study was conducted to assess if there was any difference in the levels of coated-platelets in patients with primary intr...
Applicability of the long-term uncertainty in measurement (LTUM) method for analytical performance assessment in clinica...
Published in Cytometry. Part B, Clinical cytometry
The estimation of uncertainty in measurement for quantitative analyses is an international obligation of the ISO15189 standard for laboratories. The most widespread method is the Internal Quality Control and External Quality Assessment (IQC + EQA). We compared two methods to assess uncertainty in measurement for the quantification of the number of ...
Erroneous expression of NKG2D on granulocytes detected by phycoerythrin-conjugated clone 149810 antibody.
Published in Cytometry. Part B, Clinical cytometry
The activating Natural killer group 2 member D (NKG2D) receptor is typically expressed on NK cells, CD8 T lymphocytes, γδ T cells and small subsets of CD4 T lymphocytes. During the course of an extensive flow cytometry phenotyping of immune cells in the peripheral blood of patients with glioblastoma multiforme (GBM) we noticed an unexpected express...
Combined use of different antibody clones improves the efficiency of human leukocyte antigen B27 detection by flow cytom...
Published in Cytometry. Part B, Clinical cytometry
Human leukocyte antigen B27 (HLA-B27) is an major histocompatibility complex Class I cell surface antigen that shows strong association with spondylarthropathies. Although polymerase chain reaction (PCR) is the gold standard method for HLA-B27 detection, monoclonal antibodies, and flow cytometric analysis is also frequently used. We aimed to compar...
CD4+ T-cell large granular lymphocytic leukemia with STAT3 mutation and neutropenia.
Published in Cytometry. Part B, Clinical cytometry
Increased TOX expression concurrent with PD-1, Tim-3, and CD244 expression in T cells from patients with acute myeloid l...
Published in Cytometry. Part B, Clinical cytometry
T cell dysregulation is a common event in leukemia. Recent findings have indicated that aberrant expression of immune checkpoint proteins may be associated with disease relapse and progression in acute myeloid leukemia (AML). TOX, a transcription factor in the HMG-box protein superfamily, was found to be a potential target for immunotherapy not onl...
