Chaibakhsh, Naz Rahman, Mohd Basyaruddin Abdul Basri, Mahiran Salleh, Abu Bakar Abd-Aziz, Suraini
Published in
Computational and Structural Biotechnology Journal
Dimethyl adipate (DMA) was synthesized by immobilized Candida antarctica lipase B-catalyzed esterification of adipic acid and methanol. To optimize the reaction conditions of ester production, response surface methodology was applied, and the effects of four factors namely, time, temperature, enzyme concentration, and molar ratio of substrates on p...
Hemayatkar, Mahdi Mahboudi, Fereidoun Majidzadeh-A, Keivan Davami, Fatemeh Vaziri, Behrouz Barkhordari, Farzaneh Adeli, Ahmad Mahdian, Reza Davoudi, Noushin
Published in
Computational and Structural Biotechnology Journal
Recombinant tissue plasminogen activator (rt-PA) is one of the most important thrombolytic agents for treating cardiovascular obstructions such as stroke. Glycoprotein rt-PA is a serine protease, consisting of 527 amino acids of which 35 are cysteine residues. A variety of recombinant protein expression systems have been developed for heterologous ...
Jamil, Kaiser Jayaraman, Archana Rao, Raghunatha Raju, Suryanarayana
Published in
Computational and structural biotechnology journal
Notch signaling plays a critical role in cell fate determination and maintenance of progenitors in many developmental systems. Notch receptors have been shown to be expressed on hematopoietic progenitor cells as well as to various degrees in peripheral blood T and B lymphocytes, monocytes, and neutrophils. Our aim was to understand the protein inte...
Mahadevan, Radhakrishnan Henson, Michael A
Published in
Computational and structural biotechnology journal
Biotechnology research is traditionally focused on individual microbial strains that are perceived to have the necessary metabolic functions, or the capability to have these functions introduced, to achieve a particular task. For many important applications, the development of such omnipotent microbes is an extremely challenging if not impossible t...
Stark, Benjamin C Dikshit, Kanak L Pagilla, Krishna R
Published in
Computational and structural biotechnology journal
The hemoglobin (VHb) from Vitreoscilla was the first bacterial hemoglobin discovered. Its structure and function have been extensively investigated, and engineering of a wide variety of heterologous organisms to express VHb has been performed to increase their growth and productivity. This strategy has shown promise in applications as far-ranging a...
Jouhten, Paula
Published in
Computational and structural biotechnology journal
Cell factories are commonly microbial organisms utilized for bioconversion of renewable resources to bulk or high value chemicals. Introduction of novel production pathways in chassis strains is the core of the development of cell factories by synthetic biology. Synthetic biology aims to create novel biological functions and systems not found in na...
Grădinaru, Cristian Lopacińska, Joanna M Huth, Johannes Kestler, Hans A Flyvbjerg, Henrik Mølhave, Kristian
Published in
Computational and structural biotechnology journal
Motility studies of cells often rely on computer software that analyzes time-lapse recorded movies and establishes cell trajectories fully automatically. This raises the question of reproducibility of results, since different programs could yield significantly different results of such automated analysis. The fact that the segmentation routines of ...
Spencer, Paige S Barral, José M
Published in
Computational and structural biotechnology journal
The genetic code is said to be redundant in that the same amino acid residue can be encoded by multiple, so-called synonymous, codons. If all properties of synonymous codons were entirely equivalent, one would expect that they would be equally distributed along protein coding sequences. However, many studies over the last three decades have demonst...
Verma, Rajni Schwaneberg, Ulrich Roccatano, Danilo
Published in
Computational and structural biotechnology journal
The combination of computational and directed evolution methods has proven a winning strategy for protein engineering. We refer to this approach as computer-aided protein directed evolution (CAPDE) and the review summarizes the recent developments in this rapidly growing field. We will restrict ourselves to overview the availability, usability and ...
Papagianni, Maria
Published in
Computational and structural biotechnology journal
Lactic acid bacteria (LAB) are receiving increased attention for use as cell factories for the production of metabolites with wide use by the food and pharmaceutical industries. The availability of efficient tools for genetic modification of LAB during the past decade permitted the application of metabolic engineering strategies at the levels of bo...
