Manna, Sudeshna Sontakke, Vyankat A Srivatsan, Seergazhi G
Published in
Chembiochem : a European journal of chemical biology
Development of versatile probes that can enable the study of different conformations and recognition properties of therapeutic nucleic acid motifs by complementing biophysical techniques can greatly aid nucleic acid analysis and therapy. Here, we report the design, synthesis and incorporation of an environment-sensitive ribonucleoside analogue, whi...
Cohen, Zachary R Kessenich, Brennan L Hazra, Avijit Nguyen, Julia Johnson, Richard S MacCoss, Michael J Lalic, Gojko Black, Roy A Keller, Sarah L
Published in
Chembiochem : a European journal of chemical biology
Cycles of dehydration and rehydration could have enabled formation of peptides and RNA in otherwise unfavorable conditions on the early Earth. Development of the first protocells would have hinged upon colocalization of these biopolymers with fatty acid membranes. Using atomic force microscopy, we find that a prebiotic fatty acid (decanoic acid) fo...
Gama, Simanga R Stankovic, Toda Hupp, Kendall Al Hejami, Ahmed McClean, Mimi Evans, Alysa Beauchemin, Diane Hammerschmidt, Friedrich Pallitsch, Katharina Zechel, David L
...
Published in
Chembiochem : a European journal of chemical biology
The fungal metabolite Fosfonochlorin features a chloroacetyl moiety that is unusual within known phosphonate natural product biochemistry. Putative biosynthetic genes encoding Fosfonochlorin in Fusarium and Talaromyces spp. were investigated through reactions of encoded enzymes with synthetic substrates and isotope labelling studies. We show that t...
Hu, Zhenyi Crews, Craig M
Published in
Chembiochem : a European journal of chemical biology
Proteolysis-targeting chimeras (PROTACs), an emerging paradigm-shifting technology, hijacks the ubiquitin-proteasome system for targeted protein degradation. PROTACs induce ternary complexes between an E3 ligase and POI, and this induced proximity leads to polyUb chain formation on substrates and eventual proteasomal-mediated POI degradation. PROTA...
Esposito, Nikolas Konas, David W Goodey, Nina M
Published in
Chembiochem : a European journal of chemical biology
Tuberculosis (TB), caused by the pathogen Mycobacterium tuberculosis, affects millions of people worldwide. Several TB drugs have lost efficacy due to emerging drug resistance and new anti-TB targets are needed. Recent research suggests that indole-3-glycerol phosphate synthase (IGPS) in M. tuberculosis (MtIGPS) could be such a target. IGPS is a (β...
Cordon, Marie B Jacobsen, Kristian M Nielsen, Cecilie S Hjerrild, Per Poulsen, Thomas B
Published in
Chembiochem : a European journal of chemical biology
The identification of growth inhibitory compounds with the ability to selectively target the cellular oxygenation state may be of therapeutic interest. Here, a phenotypic screen of a covalent fragment library revealed diverse compounds containing propiolamide warheads with selective toxicity for liver cancer cells in normoxic conditions. Target ide...
Sun, Ying-Jie Cheng, Liang
Published in
Chembiochem : a European journal of chemical biology
Thiamine (vitamin B1) is crucial for life and plays a central role in metabolism. It contains thiazole and pyrimidine moieties that are constructed independently and then assembled together to generate thiamine phosphate. The study of the thiazole moiety is relatively clear, but deciphering the mechanistic enzymology of thiamine pyrimidine is more ...
Tassone, Giusy Paolino, Marco Pozzi, Cecilia Reale, Annalisa Salvini, Laura Giorgi, Gianluca Orlandini, Maurizio Galvagni, Federico Mangani, Stefano Yang, Xuchun
...
Published in
Chembiochem : a European journal of chemical biology
The use of light-responsive proteins to control both living or synthetic cells, is at the core of the expanding fields of optogenetics and synthetic biology. It is thus apparent that a richer reaction toolbox for the preparation of such systems is of fundamental importance. Here, we provide a proof-of-principle demonstration that Morita-Baylis-Hill...
Hartman, Matthew C T
Published in
Chembiochem : a European journal of chemical biology
In this comprehensive review, I focus on the twenty E. coli aminoacyl-tRNA synthetases and their ability to charge non-canonical amino acids (ncAAs) onto tRNAs. The promiscuity of these enzymes has been harnessed for diverse applications including understanding and engineering of protein function, creation of organisms with an expanded genetic code...
Gilboa, Tal Ogata, Alana F Walt, David R
Published in
Chembiochem : a European journal of chemical biology
Enzymes can be used as biomarkers for a variety of diseases. However, profiling enzyme activity in clinical samples is challenging due to the heterogeneity in enzyme activity, and the low abundance of the target enzyme in biofluids. Single-molecule methods can overcome these challenges by providing information on the distribution of enzyme activiti...
