Sawaguchi, S Yue, B Y Chang, I L Wong, F Higginbotham, E J
Published in
Cellular and molecular biology
The trabecular meshwork, a specialized tissue in the anterior chamber of the eye, plays a major role in the regulation of aqueous humor outflow. We studied the effects of ascorbic acid, a significant component in the aqueous humor, on gene expression of type I collagen in cultures of bovine trabecular meshwork cells. These cells were plated for 6 d...
Gopas, J Ono, M Princler, G Smith, M R Tainsky, M A Siddiqui, M A Wishniak, O Segal, S Kuwano, M Kung, H F
...
Published in
Cellular and molecular biology
EGF receptors are found on the surface of most cells, usually with high and low binding affinities. To investigate functional relationships between EGF (EGF-like growth factors) and oncogenes we have characterized the expression of the epidermal growth factor receptor (EGFr) in H-Ras, v-Myc, and H-Ras-v-Myc transformed Balb/3T3 cells. H-Ras cells s...
Boutjdir, M Morgenstern, D Wei, Y Restivo, M el Sherif, N Siddiqui, M A
Published in
Cellular and molecular biology
Previous studies have shown degradation of cardiac structural proteins and disruption of the sarcolemma as a result of acute myocardial infarction. However, there is no evidence to date on changes in sarcolemmal membrane proteins induced by experimental subacute myocardial infarction. We studied subepicardial layers overlying myocardial infarct 4 d...
Banerjee, S Ganapathi, R Ghosh, L Yu, C L
Published in
Cellular and molecular biology
The murine melanoma tumor cells, B16-BL6, are a recognized model for experimental and spontaneous metastasis. B16-BL6 cells express a lower metastatic phenotype upon acquisition of resistance to adriamycin. Using this novel system, the role of ras, c-myc, and multidrug-resistant gene (mdr1) expression in the metastatic and drug-resistant phenotype ...
Skubitz, K M Ehresmann, D D
Published in
Cellular and molecular biology
The growth of new blood vessels plays an important role in the pathogenesis of several diseases including cancer, diabetes, and arthritis. Beta-cyclodextrin tetradecasulfate, when administered with an appropriate steroid inhibits angiogenesis, and can stimulate angiogenesis when given alone. The regulation of angiogenesis is not well understood, an...
Guarini, L Su, Z Z Zucker, S Lin, J Grunberger, D Fisher, P B
Published in
Cellular and molecular biology
The active component of the honeybee hive product propolis, caffeic acid phenethyl ester (CAPE), has been shown to display increased toxicity toward various oncogene-transformed cell lines in comparison with their untransformed counterparts (Su et al., 4: 231-242, 1991). This observation provides support for the concept that it is the transformed p...
Sharp, S B Vazquez, A Theimer, M Silva, D K Muscati, S R Sylber, M Mogassa, M
Published in
Cellular and molecular biology
Little is known about the factors which regulate vascular smooth muscle (vsm) actin gene expression during skeletal myogenesis in culture. We have therefore looked for differences in the levels of accumulation of vsm actin mRNA among six mouse cell lines differing in apparent myogenic potential or in the complement of myogenesis determination genes...
Crockett, C O Guede-Guina, F Pugh, D Vangah-Manda, M Robinson, T J Olubadewo, J O Ochillo, R F
Published in
Cellular and molecular biology
In our search for therapeutic agents from natural sources with potential for the treatment of opportunistic infections in patients afflicted with acquired immunodeficiency syndrome (AIDS), we investigated antibacterial and antifungal activities of water extracts of Cassia alata (C. alata). The extracts are traditionally used in Ivory Coast, West Af...
Rahman, M A Brot, N Weissbach, H
Published in
Cellular and molecular biology
The enzyme peptide methionine sulfoxide reductase catalyzes the conversion of methionine sulfoxide residues in proteins to methionine. The 636 nucleotide coding region of the peptide methionine sulfoxide reductase gene has been amplified from a genomic clone using the polymerase chain reaction and the product was subcloned into plasmid pGEX-2T down...
Kumar, A Shafiq, S Wadgaonkar, R Stracher, A
Published in
Cellular and molecular biology
Intracellular calcium levels play an important role in myofibril disintegration and regeneration of muscle fibers. Earlier studies have shown that the calcium activated protease, calpain, is involved in the removal of Z-discs from myofibrils of striated muscle and the tripeptide-aldehyde, leupeptin, which is an inhibitor of calpain, inhibits this a...
