Ma, Yinxing Moors, Alexis Camougrand, Nadine Dokudovskaya, Svetlana
Published in
Cellular and Molecular Life Sciences
The major signaling pathway that regulates cell growth and metabolism is under the control of the target of rapamycin complex 1 (TORC1). In Saccharomyces cerevisiae the SEA complex is one of the TORC1 upstream regulators involved in amino acid sensing and autophagy. Here, we performed analysis of the expression, interactions and localization of SEA...
Stefanović, V. Vlahović, P. Mitić-Zlatković, M.
Published in
Cellular and Molecular Life Sciences CMLS
Aminopeptidase N was demonstrated in human dermal fibroblasts as an ectoenzyme. The enzyme has wide substrate specificity, with a Km of 0.63 mM and Vmax of 338 nmol min−1 mg−1. Addition of fetal calf serum to the culture medium increased aminopeptidase N activity up to 63% by 10% serum in a 48-h culture. Treatment of fibroblasts by dexamethasone in...
Traving, C. Schauer, R.
Published in
Cellular and Molecular Life Sciences CMLS
Sialic acids represent a family of sugar molecules with an unusual and highly variable chemical structure that are found mostly in the terminal position of oligosaccharide chains on the surface of cells and molecules. These special features enable them to fulfil several important and even diametrical biological functions. Because of the great impor...
Thébaud, B. Arnal, J.-F. Mercier, J. C. Dinh-Xuan, A.-T.
Published in
Cellular and Molecular Life Sciences CMLS
Inhaled nitric oxide (NO) is used to treat various cardiopulmonary disorders associated with pulmonary hypertension. The rationale is based on the fact that NO, given by inhalation, only dilates those pulmonary vessels that perfuse well-ventilated lung units. As a result, pulmonary gas exchange is improved while pulmonary vascular resistance is red...
Kuhn, J. H. Li, W. Choe, H. Farzan, M.
Published in
Cellular and Molecular Life Sciences CMLS
Cellular entry of enveloped viruses is often dependent on attachment proteins expressed on the host cell surface. Viral envelope proteins bind these receptors, and, in an incompletely understood process, facilitate fusion of the cellular and viral membranes so as to introduce the viral core into the cytoplasm. Only a small fraction of viral recepto...
Xiao, X. Dimitrov, D. S.
Published in
Cellular and Molecular Life Sciences CMLS
The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. Recent rapid advances in our knowledge of the structure and function of this protein have lead to the development of a num...
Carralot, J.-P. Probst, J. Hoerr, I. Scheel, B. Teufel, R. Jung, G. Rammensee, H.-G. Pascolo, S.
Published in
Cellular and Molecular Life Sciences CMLS
In the context of developing a safe genetic vaccination strategy we tested and studied globin-stabilized mRNA-based vaccination in mice. This vaccination strategy has the advantages of genetic vaccination (easy production, adaptability to any disease and inexpensive storage when lyophilized), but not the drawbacks of DNA vaccination (long-term unco...
Warner, F. J. Smith, A. I. Hooper, N. M. Turner, A. J.
Published in
Cellular and Molecular Life Sciences CMLS
Angiotensin-converting enzyme-2 (ACE2) is the first human homologue of ACE to be described. ACE2 is a type I integral membrane protein which functions as a carboxypeptidase, cleaving a single hydrophobic/basic residue from the C-terminus of its substrates. ACE2 efficiently hydrolyses the potent vasoconstrictor angiotensin II to angiotensin (1–7). I...
Smith, A. I. Turner, A. J.
Published in
Cellular and Molecular Life Sciences CMLS
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Cinatl Jr., J. Hoever, G. Morgenstern, B. Preiser, W. Vogel, J.-U. Hofmann, W.-K. Bauer, G. Michaelis, M. Rabenau, H. F. Doerr, H. W.
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Published in
Cellular and Molecular Life Sciences CMLS
To identify a model for the study of intestinal pathogenesis of severe acute respiratory syndrome (SARS) we tested the sensitivity of six human intestinal epithelial cell lines to infection with SARS coronavirus (SARS-CoV). In permissive cell lines, effects of SARS-CoV on cellular gene expression were analysed using high-density oligonucleotide arr...
