Published in Cell reports
High-dimensional data, such as those generated by single-cell RNA sequencing (scRNA-seq), present challenges in interpretation and visualization. Numerical and computational methods for dimensionality reduction allow for low-dimensional representation of genome-scale expression data for downstream clustering, trajectory reconstruction, and biologic...
Published in Cell reports
Although modulating the neuronal cytoskeleton has been deemed a promising approach to enhance mammalian axon regeneration, only a few studies have shown convincing results, especially in the central nervous system. Wang et al. demonstrate that the deletion of non-muscle myosin II sufficiently induces significant mammalian CNS axon regeneration in v...
Published in Cell reports
Banks et al. capture positional information for subunits within Sin3/HDAC complexes by combining crosslinking and high-resolution mass spectrometry. This information is then used to guide docking of Sin3 subunit structures to develop a model of a Sin3/HDAC complex sub-structure.
Published in Cell reports
Published in Cell reports
Martin et al. advance the use of the Collaborative Cross (CC) for studying adaptive immune responses. They demonstrate that the CC better models variation in T cell responses seen in outbred mice and humans and that it can uncover genes linked to generation of qualitatively distinct memory cells following infection.
Published in Cell reports
Levy et al. record populations of neurons in visual cortex responding to drifting gratings. Pairwise correlations, summarized as functional networks, are specific for each direction. Reliably responding neurons, i.e., tuned and untuned neurons, are differentially located in networks. Both classes of neurons and their connections contribute to the c...
Published in Cell reports
Justice et al. show that WIZ functions with CTCF and cohesin in the structural regulation of DNA loops. Aberrant WIZ function causes many changes in gene expression, including at DNA loops important for regulating stem cell identity genes.
Published in Cell reports
A preovulatory LH surge induces the expression of PGR and the production of PGE2, a proinflammatory prostaglandin, in preovulatory granulosa cells. Park et al. show that PGR then suppresses PGE2 synthesis by decreasing the expression of PTGS2. Such PGR-driven termination temporarily confines ovulatory inflammation within a short period.
Published in Cell reports
During nematode development, the olfactory neuron gene olrn-1 programs odorant receptor expression in AWC neurons. Foster et al. show that olrn-1 also functions in AWC neurons to suppress innate immune defenses in the intestine. These data reveal an unexpected connection between olfactory receptor development and innate immunity.
Published in Cell reports
Kissinger et al. study perceptual experience-dependent visual cortical oscillations in Fragile X mice using extracellular electrophysiology, revealing decreased oscillatory magnitudes and altered temporal profiles in Fragile X mice. Additionally, they perform a cross-layer functional connectivity analysis of these data and channelrhodopsin-2 assist...
