Published in Cell reports
Bollimunta et al. demonstrate head-mounted recordings of cellular-resolution calcium dynamics from large populations of premotor cortical neurons in behaving macaques. Imaging is stable over several months, allowing longitudinal tracking of neurons over time. Calcium dynamics exhibit selectivity for reach direction and could be used to decode the a...
Published in Cell reports
During dosage compensation, the expression from the single X chromosome of male flies is upregulated to match the two X’s of females. Aleman et al. show that a nuclear pore protein functions in achieving this transcriptional precision and restricts the upregulation of the male X to the required 2-fold.
Published in Cell reports
Motor neuron hyperexcitability is observed in both ALS patients and their iPSC-derived neurons. Combining a high-content live imaging excitability phenotypic assay, high-throughput screening against a cross-annotated chemogenomic library, and bioinformatic enrichment analysis, Huang et al. identify targets modulating the hyperexcitability of ALS pa...
Published in Cell reports
Sun et al. examine fibronectin receptor biophysics in live embryos and find that many of these integrins are never activated along the zebrafish somite boundary. Integrins α5β1 and αVβ1 are activated, but integrins αVβ3, αVβ5, and αVβ6 exhibit no activity. Integrin intra-heterodimer affinity appears to determine how readily an integrin is activated...
Published in Cell reports
Hartmann et al. demonstrate that MRs regulate baseline FKBP5 expression in the hippocampus. This regulation leads to a modification of GR sensitivity to glucocorticoids during acute stress. The results suggest that FKBP5 acts as a key modulator of HPA axis activity by mediating the fine-tuning of hippocampal MR:GR balance.
Published in Cell reports
Khan et al. show that SIRT6, a nuclear sirtuin, regulates the transcription of fatty acid transporter genes in the heart through the PPARγ transcription factor in a deacetylase-independent manner. Notably, deficiency of SIRT6 causes increased fatty acid uptake and lipid accumulation in the heart.
Published in Cell reports
The molecular mechanisms by which beta-amyloid (Aβ) damages synapses are poorly understood. Dore et al. show that increasing PSD-95 synaptic contentprotects synapses from Ab-induced synaptic deficits by reducing NMDA receptor metabotropic function.
Published in Cell reports
Cox10 encodes a key component of complex IV in the mitochondrial electron transport chain. Mah-Som et al. use an inducible model of Cox10 deletion in type I ILCs to demonstrate that COX10 is required for antigen-specific expansion of NK cells during viral infection but not cytokine-driven proliferation.
Published in Cell reports
The mechanisms controlling macrophage metabolism during bacterial infections remain poorly characterized. Olson et al. show that type I interferon, rather than direct bacterial virulence factors, restrains macrophage metabolism during Mycobacterium tuberculosis infection. IFNβ itself prevents the shift to aerobic glycolysis in inflammatory macropha...
Published in Cell reports
Hamilton et al. demonstrate that engineered virus-like particles enable simultaneous CRISPR-Cas9 genetic knockout and the anti-tumor reprogramming of T cells. By outwardly making virus-like particles resemble HIV-1, the authors additionally demonstrate specific targeting of CD4+ T cells for genome editing within a mixed cell population.
