Heuser, Vanina Dahlström de Andrade, Vanessa Moraes Peres, Alessandra Gomes de Macedo Braga, Luisa Maria Bogo Chies, José Arthur
Published in
Cell biology international
We have investigated the normal variations in basal DNA damage detected by Comet assay in leukocytes and micronucleated erythrocytes (MNE) using the Micronucleus test (MN) in peripheral blood cells from 45 female and male mice from different age groups (newborns, 3.5, 12, and 104 weeks) to clarify age and sex-related changes. Comparison of basal DN...
Pathmanathan, Sevvel Barnard, Emma Timson, David J
Published in
Cell biology international
A split-EGFP based bimolecular fluorescence complementation (BiFC) assay has been used to detect interactions between the Saccharomyces cerevisiae cytoskeletal scaffolding protein Iqg1p and three targets: myosin essential light chain (Mlc1p), calmodulin (Cmd1p) and the small GTPase Cdc42p. The format of the BiFC assay used ensures that the proteins...
Park, Ju Youn Chang, Jae-Ho Bae, Keum Seok Lee, Kyoung Ho Choi, Sun Ju Park, Joo Young Ryang, Yong Suk Kim, Soo-Ki
Published in
Cell biology international
The present study was to see whether echinomycin-induced apoptosis would be NF-kappaB-dependent and if so, whether echinomycin would activate or inhibit NF-kappaB as well as resultant chemokine IL-8 expression. In HT-29 cells echinomycin activated NF-kappaB in time-dependent manner. EMSA in the presence of antibodies specific for p50 and p65 subuni...
Liu, Li Wang, Shuang Zhang, Qingling Ding, Yanqing
Published in
Cell biology international
Tiam1 (T-cell lymphoma invasion and metastasis-inducing protein 1), a guanine nucleotide exchange factor that activates Rac, is a colorectal cancer metastasis-related gene. In this study, we aimed to better understand the mechanism underlying Tiam1-mediated metastasis. We applied gene microarray and proteome analysis and compared expression of gene...
Hamamura, Kazunori Zhang, Ping Yokota, Hiroki
Published in
Cell biology international
Insulin-like growth factor-2 (IGF2) is essential for fetal development as well as maintenance of adult organs such as brain and liver. Although genetic polymorphisms of IGF2 are linked to cytoskeletal variations little is known about the mechanisms of IGF2 action in proliferation and differentiation of chondrocytes for skeletal growth. A genome-wid...
Lin, Dingsheng Feng, Jie Chen, Weishan
Published in
Cell biology international
Detachment of adherent cells from extracellular matrix results in apoptosis, a process termed "anoikis". Resistance to anoikis is implicated in the progression of many malignancies by facilitating the migration and eventual colonization of distant sites. Human kidney epithelial cells 293T, human osteoblast cells hFOB 1.19 and human osteosarcoma cel...
Tang, Youchao Tang, Wei Lin, Yunfeng Long, Jie Wang, Hang Liu, Lei Tian, Weidong
Published in
Cell biology international
Autogenous cells transfected with hBMP-2 promote bone formation in osteoporotic rats. BMSC-mediated BMP-2 gene therapy used in conjunction with bone tissue engineering may be used to successfully treat bone defects in osteoporotic rats. This method provides a powerful tool for bone regeneration and other tissue engineering.
Tiwari, Anand K Alone, Debasmita P Roy, Jagat K
Published in
Cell biology international
Rab11, a small GTP binding protein involved in vesicular trafficking, has emerged as a key player in regulating various cellular events during Drosophila development and differentiation. In our earlier study a P-insertion line, Rab11mo, was established as a new hypomorphic allele of Rab11 gene, showing degenerated eye phenotype, bristle abnormaliti...
Yu, Hong-Nu Lee, Young-Rae Noh, Eun-Mi Lee, Kyung-Sun Kim, Jong-Suk Song, Eun-Kyung Han, Myung-Kwan Lee, Yong-Chul Kwon, Kang-Beom Lee, Seung-Jin
...
Published in
Cell biology international
Peroxisome proliferator-activated receptor gamma (PPARgamma) ligands inhibit cell proliferation and induce apoptosis in cancer cells. Here we wished to determine whether the PPARgamma ligand induces apoptosis and cell cycle arrest of the MDA-MB-231 cell, an estrogen receptor alpha negative breast cancer cell line. The treatment of MDA-MB-231 cell w...
Mampumbu, André Roberto Mello, Maria Luiza S
Published in
Cell biology international
Melipona quadrifasciata and Melipona rufiventris are stingless bee species which present low and high heterochromatin content, respectively, on their mitotic chromosomes as assessed visually after a C-banding assay. However, these species do not show differences in the C-banding responses of their Malpighian tubule interphase nuclei. In the present...
