Park, Young-Guk Kim, Kyung-Woon Song, Kwon-Ho Lee, Ji-Min Hong, Jeong-Jin Moon, Sungk-Kwon Kim, Cheorl-Ho
Published in
Cell biology international
Combinatory responses of proinflamamtory cytokines have been examined on the nitric oxide-mediated function in cultured mouse calvarial osteoblasts. Interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) induced iNOS gene expression and NO production, although these actions were inhibited by L-NG-monomethylarginine (L-NMMA) and de...
Yin, Ping Kawamura, Tomoya He, Meilan Vanaja, Donkena Krishna Young, Charles Y F
Published in
Cell biology international
This study was to investigate the effect of phenethyl isothiocyanate (PEITC), a constituent of many edible cruciferous vegetables, on the expression of alpha- and beta-tubulins, which are the main components of microtubules in prostate cancer cells. Flow cytometry, light microscopy and western blot were used to study the cell cycle distribution, mo...
Li, Jun-Hui Ma, Qing-Yong Shen, Su-Gang Hu, Heng-Tong
Published in
Cell biology international
Stimulation of mice dorsal root ganglion neurons (DRGNs) activity by human pancreatic cancer (PanCa) cell line Mia PaCa-2 and its potential molecule mechanism has been investaged. DRGNs were cultured alone or along with the MIA PaCa-2. The effects of MIA PaCa-2 to DRGNs were determined by neurofilament (NF) immunocytochemical and Nissl staining. EL...
Iakimova, Elena T Woltering, Ernst J Kapchina-Toteva, Veneta M Harren, Frans J M Cristescu, Simona M
Published in
Cell biology international
Our aim was to investigate the ability of cadmium to induce programmed cell death in tomato suspension cells and to determine the involvement of proteolysis, oxidative stress and ethylene. Tomato suspension cells were exposed to treatments with CdSO(4) and cell death was calculated after fluorescein diacetate staining of the living cells. Ethylene ...
Liu, Mingna Gong, Bo Qi, Zhi
Published in
Cell biology international
The Kv2.1 potassium channel is a principal component of the delayed rectifier I(K) current in the pyramidal neurons of cortex and hippocampus. We used whole-cell patch-clamp recording techniques to systemically compare the electrophysiological properties between the native neuronal I(K) current of cultured rat hippocampal neurons and the cloned Kv2...
Han, Yong Hwan Kim, Sung Zoo Kim, Suhn Hee Park, Woo Hyun
Published in
Cell biology international
2,4-Dinitrophenol (DNP) is an uncoupler of oxidative phosphorylation in mitochondria. Here, we investigated the in vitro effect of DNP on apoptosis and the involvement of reactive oxygen species (ROS) in As4.1 juxtaglomerular cell death. Dose- and time-dependent induction of apoptosis was evidenced by flow cytometric detection of sub-G1 DNA content...
Jiang, Ruzhang Huang, Bing Jin, Chenjin Song, Ge Zhong, Xiufeng Yuan, Jing Xiang, Peng He, Yuan Liu, Bingqian Sun, Xuerong
...
Published in
Cell biology international
Microinjection of adult stem cells (ASCs) into blastocysts provides a classic model for studying ASC plasticity. To explore the molecular mechanisms that govern the reprogramming of ASCs, we evaluated the experimental model through microinjection of human epidermal stem cells (hEpiSCs) into mouse blastocysts. Mouse blastocysts underwent regular emb...
Aichinger, Michael C Ortbauer, Martina Reipert, Siegfried Zauner, Wolfgang Bogner, Péter Froschauer, Elisabeth Nowikovsky, Karin Lingnau, Karen von Gabain, Alexander Schweyen, Rudolf
...
Published in
Cell biology international
We have monitored the effects of KLKL(5)KLK (KLK), a derivative of a natural cationic antimicrobial peptide (CAP) on isolated membrane vesicles, and investigated the partition of the peptide within these structures. KLK readily interacted with fluorescent dyes entrapped in the vesicles without apparent pore formation. Fractionation of vesicles reve...
Felismino, Mariana Ferrari Pagliarini, Maria Suely do Valle, Cacilda Borges
Published in
Cell biology international
Several mutations are known to alter the normal progression of meiosis and can be correlated with defects in microtubule distribution. The dv mutation affects the spindle organization and chromosomes do not converge into focused poles. Two Brachiaria hybrids presented the phenotypic expressions of dv mutation but exhibited many more details in the ...
Zhang, Hai-Ying Liu, Yi-Heng Wang, Hong-Quan Xu, Jie-Hua Hu, Hai-Tao
Published in
Cell biology international
beta-Amyloid protein (Abeta), a major protein component of brain senile plaques in Alzheimer's disease, is known to be directly responsible for the production of reactive oxygen species (ROS) and induction of apoptosis. In this study, the protective effect of puerarin, an isoflavone purified from the radix of the Chinese herb Pueraria lobata, on Ab...
