Brahim, Souhir Aroui, Sonia Abid, Kaouthar Kenani, Abderraouf
Published in
Cell biology international
In our previous studies, we demonstrated that the deglycosylation of bleomycin-A2 (BLM-A2) does not affect the capacity of this drug to induce cell death by apoptosis in a caspase-independent manner in laryngeal cancer cells (HEp-2), but suppresses the ability of BLM-A2 to induce ROS formation. We have now investigated the consequence of BLM-A2 deg...
Bironaite, D Baltriukiene, D Uralova, N Stulpinas, A Bukelskiene, V Imbrasaite, A Kalvelyte, A
Published in
Cell biology international
Apoptosis in heart failure has been intensively investigated in vitro and in vivo. Stem cells have therapeutic value in the direct treatment of diseases, including cardiovascular disease. The main drawback of stem cell therapy is their poor survival in the diseased tissues. Since intracellular mitogen-activated protein kinases (MAPKs) actively part...
Machado, Aline Helena Araujo Pacheco Soares, Cristina da Silva, Newton Soares Moraes, Karen C M
Published in
Cell biology international
Photodynamic therapy is an attractive therapeutic procedure for a variety of neoplastic and non-neoplastic disorders that involve a tumor localizing photosensitizer that induces cytotoxicity when activated by a particular wavelength of light. Considering the acceptability of the phthalocyanine photosensitizers, we began to address initial cellular ...
Lu, Min Gong, Xingguo
Published in
Cell biology international
Exogenous oxidative stress induces cell death, but the upstream molecular mechanisms involved of the process remain relatively unknown. We determined the instant dynamic reactions of intracellular reactive oxygen species (ROS, including hydrogen peroxide (H(2)O(2)), superoxide radical (O(2)(*)(-)), and nitric oxide (NO)) in cells exposed to exogeno...
Wang, Zhao-jun Zhang, Fu-min Wang, Lian-sheng Yao, Yong-wei Zhao, Qiang Gao, Xiang
Published in
Cell biology international
Apoptosis of implanted mesenchymal stem cells (MSCs) limits the efficiency of MSC therapy. Recent studies showed the ligands of Toll-like receptors (TLRs) could control the function of these cells. We have investigated the effect of lipopolysaccharides (LPS), a ligand of TLR4, on the survival of MSCs and explored the roles of TLR4 and PI3K/Akt. H(2...
Tréguer, Karine Naye, François Thiébaud, Pierre Fédou, Sandrine Soulet, Fabienne Thézé, Nadine Faucheux, Corinne
Published in
Cell biology international
Stromal cells follow a vascular smooth muscle differentiation pathway. However, cell culture models performed from human bone marrow do not allow the obtention of a large proportion of highly differentiated smooth muscle cells (SMC) and their differentiation pathways remain unclear. We have characterized a new model of SMC differentiation from huma...
Mishra, Pradyumna Kumar Bhargava, Arpit Raghuram, Gorantla Venkata Jatawa, Suresh Kumar Akhtar, Nabila Khan, Saba Tiwari, Archana Maudar, Kewal Krishan
Published in
Cell biology international
The toxic response of cultured human colon epithelial-FHC cells to methyl isocyanate was investigated with regard to genomic instability. Qualitative and quantitative assessments of the extent of phosphorylation of DNA damage signaling factors such as ATM, gammaH2AX and p53, was increased in treated cells compared to controls. At the same time, man...
Pandey, Alok Singh, Namita Gupta, S C Rana, J S Gupta, Neelam
Published in
Cell biology international
Relative mRNA transcript expression of insulin-like growth factors, IGF-1, IGF-2 and their receptors, IGF-1R and IGF-2R, was studied in SCNT and IVF buffalo embryos at different developmental stages using SYBR green with real-time PCR. SCNT embryos were produced by enucleating IVM oocytes and transferring granulosa cells (passage 5) followed by the...
Rodríguez de la Fuente, Abraham O Alcocer-González, Juan M Antonio Heredia-Rojas, J Balderas-Candanosa, Isaías Rodríguez-Flores, Laura E Rodríguez-Padilla, Cristina Taméz-Guerra, Reyes S
Published in
Cell biology international
We have evaluated the effect of 60 Hz sinusoidal magnetic fields (MF) at 8 and 8 microT on expression of the luciferase gene contained in a gene construct labelled as Electromagnetic Field-plasmid (pEMF). The vector included the hsp70 promotor containing the 3 nCTCTn sequences previously described for the induction of hsp70 expression by magnetic f...
Reipert, Siegfried Kotisch, Harald Wysoudil, Bhuma Neumüller, Josef
Published in
Cell biology international
A novel organelle-like membrane specialization has been found in an epithelial cell line. Characteristically, the helical membrane arrays (referred to hereafter as TUHMAs) are organized around tubular, proteinaceous electron-dense cores of 80 nm in diameter. Depending on the cell status, up to 8 of these cores provide the basis for an intermingled ...
