Sjölander, Johanna J Sunnerhagen, Per
Published in
Cell biology international
Genetic analysis has strongly implicated human FHIT (Fragile Histidine Triad) as a tumor suppressor gene, being mutated in a large proportion of early-stage cancers. The functions of the FHIT protein have, however, remained elusive. Here, we investigated aph1+ , the fission yeast homolog of FHIT, for functions related to checkpoint control and oxid...
Xu, Ying Tian, Hu Cheng, Jialin Liang, Shubin Li, Teng Liu, Ju
Published in
Cell Biology International
Telocytes had been identified as a peculiar stromal cell type implicated in tissue homeostasis and the development and pathophysiology of diseases. Telocyte existed in most organs and tissues in humans and animals. However, few studies have examined telocytes in ApoE gene deficient mice. In our studies, we verified the existence, the morphology and...
Hu, Jun Liu, Te Zhang, Zhuang Xu, Yawei Zhu, Fu
Published in
Cell biology international
Oxidized low-density lipoprotein (ox-LDL) can damage vascular endothelial cells and cause atherosclerosis, but its epigenetic regulatory mechanism has not been fully elucidated. We show that ox-LDL induced significant apoptosis and loss of function in human umbilical vascular endothelial cells (HUVECs). At the same time, ox-LDL significantly decrea...
Honjo, Yasuko Ichinohe, Tatsuo
Published in
Cell biology international
Animal cells constantly receive information about and respond to environmental factors, including ionizing radiation. Although it is crucial for a cell to repair radiation-induced DNA damage to ensure survival, cellular responses to radiation exposure during early embryonic development remain unclear. In this study, we analyzed the effects of ioniz...
Li, Caixia Zhao, Bing Lin, Caizhao Gong, Zhiping An, Xiaoxia
Published in
Cell biology international
This study aimed to investigate the effects of triggering receptor expressed on myeloid cell-2 (TREM2) on the production of pro-inflammatory mediators and cytokines induced by lipopolysaccharide (LPS) in BV2 microglia. TREM2 expression or TREM2-specific siRNA were used to induce TREM2 overexpression or silencing. The BV2 cells were pre-treated with...
Zhao, Sanjun Gao, Runchi Devreotes, Peter N. Mogilner, Alex Min Zhao
Published in
Cell Biology International
Wang, Yang Tu, Wei Lou, Yuanlei Xie, An Lai, Xianliang Guo, Fei Deng, Zhifeng
Published in
Cell biology international
The effects of mesenchymal stem cells (MSCs) on proliferation and cell fate determination of neural stem cells (NSCs) have been investigated. NSCs were co-cultured with MSCs or NIH3T3 cells using an in vitro transwell system. After 4 days, immunofluorescence staining showed that the number of cells positive for the cell proliferation antigen, ki-67...
Bird, Stephen D Walker, Robert J Legge, Michael
Published in
Cell biology international
Enzymic isolation, cryopreservation and culture of cells places considerable demand on intracellular antioxidant thiol status. Thiol status is carefully regulated in the cell by the balance of reduced and oxidized thiol species, including glutathione (GSH/GSSG) and l-cysteine (l-Cys/l-CySS disulphide). These play a pivotal role in redox signaling, ...
Wang, Ping Xu, Shujun Zhao, Kai Xiao, Bingxiu Guo, Junming
Published in
Cell biology international
This study investigates the role of dysregulated cytosolic free calcium ([Ca(2+)]c) homeostasis on microtubule (MT) ring structure in apoptotic cervical cancer (HeLa) cells induced by trichosanthin (TCS), a type I ribosome inactivating protein (RIP). The TCS-induced decrease in cell viability was significantly enhanced in combination with the speci...
Yildirim, Hatice Köçkar, Feray
Published in
Cell biology international
Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase (CA) that is overexpressed in a variety of tumor types and associated with increased metastasis, giving a poor prognosis. Transcriptional regulation of transmembrane protein CAIX is complex. We describe further characterization of the 1.2kb hCA9 promoter, and the effect of TGF...
